Effects of tripterine on mRNA expression of oncogene c-myc and platelet-derived growth factor of vascular smooth muscle cells in rats

Chen Xing; Wang Luo; Feng Meifu; Zhu Guoying

doi:10.1007/BF02934059

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Effects of tripterine on mRNA expression of oncogene c-myc and platelet-derived growth factor of vascular smooth muscle cells in rats

Updated：2021-08-27

- Effects of tripterine on mRNA expression of oncogene c-myc and platelet-derived growth factor of vascular smooth muscle cells in rats
- Chinese Journal of Integrative Medicine Vol. 4, Issue 3, Pages: 197-200(1998)
- Affiliations：
  
  1. Dept. of Cardiology, Tianjin Chest Hospital,Tianjin
  2. Department. of Cardiology, First Hospital of Beijing Medical University,Beijing
  3. State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences,Beijing
- Author bio：
- Funds：
  
  Supported by Research Subject of Medicine (No. 94-1-248), Ministry of Health
- DOI：10.1007/BF02934059
  CLC：
- Published：1998，
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Chen, X., Wang, L., Feng, M. et al. Effects of tripterine on mRNA expression of oncogene c-myc and platelet-derived growth factor of vascular smooth muscle cells in rats., CJIM 4, 197–200 (1998). https://doi.org/10.1007/BF02934059

Chen Xing, Wang Luo, Feng Meifu, et al. Effects of tripterine on mRNA expression of oncogene c-myc and platelet-derived growth factor of vascular smooth muscle cells in rats. [J]. Chinese Journal of Integrative Medicine 4(3):197-200(1998)
Chen, X., Wang, L., Feng, M. et al. Effects of tripterine on mRNA expression of oncogene c-myc and platelet-derived growth factor of vascular smooth muscle cells in rats., CJIM 4, 197–200 (1998). https://doi.org/10.1007/BF02934059 DOI：

Chen Xing, Wang Luo, Feng Meifu, et al. Effects of tripterine on mRNA expression of oncogene c-myc and platelet-derived growth factor of vascular smooth muscle cells in rats. [J]. Chinese Journal of Integrative Medicine 4(3):197-200(1998) DOI： 10.1007/BF02934059.

摘要

Objective: To observe the effects of tripterine on mRNA expression of oncogene c-myc and platelet-derived growth factor (PDGF) in vascular smooth muscle cells (VSMCs) of rats.Methods: The fifth to tenth passage culture of VSMCs was used

tripterine and 20% fetal calf serum added into the medium of cultured VSMCs at concentration of 0.1 mg/L

0.2 mg/L and 0.3 mg/L after serum-free cultivation for 24 hours. The general RNA was isolated from VSMCs at 6 and 12 hours after the drug addition for detection of oncogene c-myc and PDGF mRNA respectively by dot blot hybridization. The cDNA probes were labeled by digoxin.Results: Tripterine inhibited the expression of PDGF mRNA of VSMCs

and decreased expression of oncogene cmyc mRNA in a dose-dependent manner

either vs. control.Conclusion: Tripterine can inhibit expression of oncogene c-myc and PDGF-A mRNA in VSMCs

therefore it would inhibit overproliferation of VSMCs.

Abstract

tripterine and 20% fetal calf serum added into the medium of cultured VSMCs at concentration of 0.1 mg/L

and decreased expression of oncogene cmyc mRNA in a dose-dependent manner

either vs. control.Conclusion: Tripterine can inhibit expression of oncogene c-myc and PDGF-A mRNA in VSMCs

therefore it would inhibit overproliferation of VSMCs.

关键词

tripterinevascular smooth muscle cellsoncogene c-mycplatelet-derived growth factor

Keywords

tripterinevascular smooth muscle cellsoncogene c-mycplatelet-derived growth factor

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Xiyuan Hospital, China Academy of Chinese Medical Sciences

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