Experimental study of R plasmid eliminating action of Rhizoma Coptidis on E. coli

Qun Chen; Nan-Ju Chen; Sheng-Chun Wang

doi:10.1007/BF02934260

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Experimental study of R plasmid eliminating action of Rhizoma Coptidis on E. coli

Updated：2021-08-27

- Experimental study of R plasmid eliminating action of Rhizoma Coptidis on E. coli
- Chinese Journal of Integrative Medicine Vol. 2, Issue 3, Pages: 222-224(1996)
- Affiliations：
  
  Teaching and Research Section of Microbiology, Guangdong Medical College,Zhanjiang
- Author bio：
- Funds：
  
  This subject was aided financially by the Higher Education Department of Guangdong Province
- DOI：10.1007/BF02934260
  CLC：
- Published：1996，
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Chen, Q., Chen, NJ. & Wang, SC. Experimental study of R plasmid eliminating action of Rhizoma Coptidis on E. coli., CJIM 2, 222–224 (1996). https://doi.org/10.1007/BF02934260

Qun Chen, Nan-Ju Chen, Sheng-Chun Wang. Experimental study of R plasmid eliminating action of Rhizoma Coptidis on E. coli. [J]. Chinese Journal of Integrative Medicine 2(3):222-224(1996)
Chen, Q., Chen, NJ. & Wang, SC. Experimental study of R plasmid eliminating action of Rhizoma Coptidis on E. coli., CJIM 2, 222–224 (1996). https://doi.org/10.1007/BF02934260 DOI：

Qun Chen, Nan-Ju Chen, Sheng-Chun Wang. Experimental study of R plasmid eliminating action of Rhizoma Coptidis on E. coli. [J]. Chinese Journal of Integrative Medicine 2(3):222-224(1996) DOI： 10.1007/BF02934260.

摘要

The R plasmid curing experiment was performed in vitro with E.coli strain E. 102 bearing R plasmid with multiple drug resistance as target bacteria and Rhizoma Coptidis as elimination agent

and the influence at different acting time of it on R plasmid elimination was also observed. Results showed that when the acting time was 24 hours

the cure rate of R plasmid was 2.42% and when the action time increased to 48 hours

the cure rate was elevated to 22. 57%. The missing pattern s of R plasmid might occur in diappearence of either multiple or single resistance.

Abstract

The R plasmid curing experiment was performed in vitro with E.coli strain E. 102 bearing R plasmid with multiple drug resistance as target bacteria and Rhizoma Coptidis as elimination agent

and the influence at different acting time of it on R plasmid elimination was also observed. Results showed that when the acting time was 24 hours

the cure rate of R plasmid was 2.42% and when the action time increased to 48 hours

the cure rate was elevated to 22. 57%. The missing pattern s of R plasmid might occur in diappearence of either multiple or single resistance.

关键词

Rhizoma CoptidisE. coliR plasmid elimination

Keywords

Rhizoma CoptidisE. coliR plasmid elimination

references

Mitsuhahi S. Drug resistance plasmids. Molecular and Cellular Biochemistry 1979; 26: 135–138.

Habn FE, Ciak J, Berberine, et al. Antibiotics: Mechanism of action of antimicrobial and antitumor agents. New York: Springer Verlag Berlin Herdelberg. 1975: 557–567.

Kado and Liu ST. rapid procedure for detection and isolation of large and small plasmids. J Bact 1980; 145(3): 1365–1373.

II a 1983: 3(6): 387–390.

1993;14(16): 167–169.

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