Protection of acanthopanax senticosus saponin on free radical injury induced aging of nerve cell

Yong-jin Pan; Yong-jian Gu; Xiao-su Gu

doi:10.1007/BF02934310

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Protection of acanthopanax senticosus saponin on free radical injury induced aging of nerve cell

Updated：2021-08-27

- Protection of acanthopanax senticosus saponin on free radical injury induced aging of nerve cell
- Chinese Journal of Integrative Medicine Vol. 8, Issue 3, Pages: 200-203(2002)
- Affiliations：
  
  Affilated Hospital of Nantong Medical College,Jiangsu
- Author bio：
- Funds：
  
  This work is supported by the Society Progress and Science Technology Development Item of Jiansu Province BS2000040
- DOI：10.1007/BF02934310
  CLC：
- Published：2002，
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Pan, Yj., Gu, Yj. & Gu, Xs. Protection of acanthopanax senticosus saponin on free radical injury induced aging of nerve cell., CJIM 8, 200–203 (2002). https://doi.org/10.1007/BF02934310

Yong-jin Pan, Yong-jian Gu, Xiao-su Gu. Protection of acanthopanax senticosus saponin on free radical injury induced aging of nerve cell. [J]. Chinese Journal of Integrative Medicine 8(3):200-203(2002)
Pan, Yj., Gu, Yj. & Gu, Xs. Protection of acanthopanax senticosus saponin on free radical injury induced aging of nerve cell., CJIM 8, 200–203 (2002). https://doi.org/10.1007/BF02934310 DOI：

Yong-jin Pan, Yong-jian Gu, Xiao-su Gu. Protection of acanthopanax senticosus saponin on free radical injury induced aging of nerve cell. [J]. Chinese Journal of Integrative Medicine 8(3):200-203(2002) DOI： 10.1007/BF02934310.

摘要

To study the effect of Acanthopanax senticosus saponin (ASS) on free radical injury induced neuron aging. On day 7 of fetal mice

cortical neuron primary passage cultures were divided into the normal control group

model group and ASS groups. The model group using free radical (FeSO4 plus H2O2) injury mode prepared in vivo cultured ICR mice cortical neuron aging model; ASS groups: 24 hrs before and after treated with H2O2 and FeSO4

different concentration of ASS was added

according to biochemical parameters such as lactate dehydrogenase (LDH)

superoxide dismutase (SOD) and malondialdehyde (MDA) etc. and histomorphologic change to observe the protection of ASS on aging neurons. The LDH

SOD

MDA of the model group were compared with the normal group

< 0. 01; ASS groups added 1.25 mg/100 ml

2.5 mg/100 ml

5 mg/100 ml concentration of ASS

their LDH

SOD

MDA compared with the model group π.05-0.01

the difference was significant. In medicated groups the SOD activity of oxidization injured nerve cells obviously elevated

LDH activity and MDA content apparently lowered. Microscope and scanning electron microscopic observation showed that supplemented with ASS to protect the nerve cell injury abated

part of the cellular structure tended to normalize. ASS could act against free radical toxic effect

increase the anti-oxidase activity

strengthen the protection of neuron cells. It is assumed that the effect against nerve cell aging was possibly through scavenging oxygen free radical

strengthening the stability of cell membrane

thus delaying the development of aging.

Abstract

To study the effect of Acanthopanax senticosus saponin (ASS) on free radical injury induced neuron aging. On day 7 of fetal mice

cortical neuron primary passage cultures were divided into the normal control group

different concentration of ASS was added

according to biochemical parameters such as lactate dehydrogenase (LDH)

superoxide dismutase (SOD) and malondialdehyde (MDA) etc. and histomorphologic change to observe the protection of ASS on aging neurons. The LDH

SOD

MDA of the model group were compared with the normal group

< 0. 01; ASS groups added 1.25 mg/100 ml

2.5 mg/100 ml

5 mg/100 ml concentration of ASS

their LDH

SOD

MDA compared with the model group π.05-0.01

the difference was significant. In medicated groups the SOD activity of oxidization injured nerve cells obviously elevated

LDH activity and MDA content apparently lowered. Microscope and scanning electron microscopic observation showed that supplemented with ASS to protect the nerve cell injury abated

part of the cellular structure tended to normalize. ASS could act against free radical toxic effect

increase the anti-oxidase activity

strengthen the protection of neuron cells. It is assumed that the effect against nerve cell aging was possibly through scavenging oxygen free radical

strengthening the stability of cell membrane

thus delaying the development of aging.

关键词

Acanthopanax senticosus saponinfree radicalnerve cell cultureaging

Keywords

Acanthopanax senticosus saponinfree radicalnerve cell cultureaging

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