Study on apoptosis of human acute T-lymphocyte leukemia cell line induced by allicin

Yu Chaoqin; Ling Changquan; Huang Xueqiang; Pan Ruiping; Zhang Denghai

doi:10.1007/BF02934662

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Study on apoptosis of human acute T-lymphocyte leukemia cell line induced by allicin

Updated：2021-08-27

- Study on apoptosis of human acute T-lymphocyte leukemia cell line induced by allicin
- Chinese Journal of Integrative Medicine Vol. 5, Issue 2, Pages: 126-130(1999)
- Affiliations：
  
  1. Changhai Hospital, The Second Military Medical University,Shanghai
  2. Changzheng Hospital, The Second Military Medical University,Shanghai
- Author bio：
- Funds：
- DOI：10.1007/BF02934662
  CLC：
- Published：1999，
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Chaoqin, Y., Changquan, L., Xueqiang, H. et al. Study on apoptosis of human acute T-lymphocyte leukemia cell line induced by allicin., CJIM 5, 126–130 (1999). https://doi.org/10.1007/BF02934662

Yu Chaoqin, Ling Changquan, Huang Xueqiang, et al. Study on apoptosis of human acute T-lymphocyte leukemia cell line induced by allicin. [J]. Chinese Journal of Integrative Medicine 5(2):126-130(1999)
Chaoqin, Y., Changquan, L., Xueqiang, H. et al. Study on apoptosis of human acute T-lymphocyte leukemia cell line induced by allicin., CJIM 5, 126–130 (1999). https://doi.org/10.1007/BF02934662 DOI：

Yu Chaoqin, Ling Changquan, Huang Xueqiang, et al. Study on apoptosis of human acute T-lymphocyte leukemia cell line induced by allicin. [J]. Chinese Journal of Integrative Medicine 5(2):126-130(1999) DOI： 10.1007/BF02934662.

摘要

Objective: To explore the law and role of apoptosis inducing effect of allicin on human acute T-lymphocyte leukemia cell line (6T-CEM).Methods: Terminal deoxylnucleotide transferase directed X-UTP nick and end labeling (TUNEL) assay

DNA gel electrophoresis

light microscopy and revers transcription-polymerase chair reaction (RT-PCR) were used to observe apoptosis of cells.Results: Allicin showed significant inducing effect on apoptosis of 6T-CEM cell when the concentration was 0.1 μg/ml

reaching its peak effect when the concentration was 50 μg/ml

with the apoptosis rate as 36.4%. Allicin (15 μg/ml) acted on 6T-CEM for 2 h and induced obvious cell apoptosis

the highest effect reached after 24 h of action

with the apoptosis rate as 56.9%. Actidione or actinomycin D could induce apoptosis of 6T-CEM

the allicin could not enhance their apoptosis inducing effect. Allicin could cause significant reduction of bcl-2 and bax gene expression of 6T-CEM

especially the bcl-2.Conclusion: Allicin could induce cell apoptosis of 6T-CEM with significant time and dose effect. Mechanism of the effect is through inhibiting the bcl-2 gene expression to reduce bcl-2/bax ratio.

Abstract

DNA gel electrophoresis

reaching its peak effect when the concentration was 50 μg/ml

with the apoptosis rate as 36.4%. Allicin (15 μg/ml) acted on 6T-CEM for 2 h and induced obvious cell apoptosis

the highest effect reached after 24 h of action

with the apoptosis rate as 56.9%. Actidione or actinomycin D could induce apoptosis of 6T-CEM

the allicin could not enhance their apoptosis inducing effect. Allicin could cause significant reduction of bcl-2 and bax gene expression of 6T-CEM

关键词

6T-CEM cellallicinapoptosisterminal deoxylnucleotide transferase directed X-UTP nick and end labeling mark

Keywords

6T-CEM cellallicinapoptosisterminal deoxylnucleotide transferase directed X-UTP nick and end labeling mark

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