Study on molecular mechanism of liver yang ascending syndrome in hypertension

Yiqiang Jin; Suiyu Hu; Donghong Yan; Aiping Liu; Faqing Tang; Chun Xiao; Binlai Chen; Yonghua Wang

doi:10.1007/BF02947368

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Study on molecular mechanism of liver yang ascending syndrome in hypertension

Updated：2021-08-27

- Study on molecular mechanism of liver yang ascending syndrome in hypertension
- Chinese Journal of Integrative Medicine Vol. 6, Issue 4, Pages: 244-249(2000)
- Affiliations：
  
  Institute of Integrated Traditional Chinese and Western Medicine, The Affiliated Xiangya Hospital, Hunan Medical University,Changsha
- Author bio：
- Funds：
  
  This study was funded by National Natural Sciences Foundation of China
- DOI：10.1007/BF02947368
  CLC：
- Published：2000，
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Jin, Y., Hu, S., Yan, D. et al. Study on molecular mechanism of liver yang ascending syndrome in hypertension., CJIM 6, 244–249 (2000). https://doi.org/10.1007/BF02947368

Yiqiang Jin, Suiyu Hu, Donghong Yan, et al. Study on molecular mechanism of liver yang ascending syndrome in hypertension. [J]. Chinese Journal of Integrative Medicine 6(4):244-249(2000)
Jin, Y., Hu, S., Yan, D. et al. Study on molecular mechanism of liver yang ascending syndrome in hypertension., CJIM 6, 244–249 (2000). https://doi.org/10.1007/BF02947368 DOI：

Yiqiang Jin, Suiyu Hu, Donghong Yan, et al. Study on molecular mechanism of liver yang ascending syndrome in hypertension. [J]. Chinese Journal of Integrative Medicine 6(4):244-249(2000) DOI： 10.1007/BF02947368.

摘要

Objective: To investigate the molecular mechanism of Liver Yang Ascending Syndrome (LYAS) in hypertension.Methods: The plasma norepinephrine (NE)

epinephrine (E) contents in patients with LYAS or Liver and Kidney Yin Deficiency Syndrome ( LKYDS) of hypertension and normal controls were determined by high performance liquid chromatography-electrochemical detector (HPLC-ECD). The polymorphism of tyrosine hydroxylase (TH) gene in the three groups were analysed by Southern Blot assay. The polymorphism of TH and monoamine oxidation A(MAO-A)

monoamine oxidation B(MAO-B) gene microsatellite in these groups were analysed by polymerase chain reaction (PCR)-SSCP. At the same time

the LYAS model was established by administering Aconitum preparation (AP) to spontaneous hypertension rats (SHR). The adrenal gland medulla of the experimental animal was examined for their TH expression at the protein level after ABC immuno-histochemical staining with the TH monoclonal antibody (McAb). The adrenal TH gene mRNA expression was demonstrated by in situ hybridization with the synthetized TH oligonucleic acid probe. The results of immuno-histochemistry and in situ hybridization were analyzed with the image analysis system ( IAS).Results: The plasma NE and E contents in patients with LYAS were more obviously increased than those in LKYDS and normal controls. The TH gene amplified and the type A1 TH microsatellite D11S4046 was markedly higher in the LYAS group than those in the other groups. The TH mRNA protein expression in the adrenal tissue of the AP induced LYAS model in SHR elevated.Conclusion: The LYAS of hypertension has the characters as amplified TH gene and increased TH mRNA and protein expression

which suggested that the overex-pression of TH is probably the mechanism of LYAS in hypertension.

Abstract

Objective: To investigate the molecular mechanism of Liver Yang Ascending Syndrome (LYAS) in hypertension.Methods: The plasma norepinephrine (NE)

monoamine oxidation B(MAO-B) gene microsatellite in these groups were analysed by polymerase chain reaction (PCR)-SSCP. At the same time

which suggested that the overex-pression of TH is probably the mechanism of LYAS in hypertension.

关键词

hypertensionLiver Yang Ascending SyndromeLiver and Kidney Yin Deficiency SyndromeTyrosine Hydroxylasemolecular mechanism

Keywords

hypertensionLiver Yang Ascending SyndromeLiver and Kidney Yin Deficiency SyndromeTyrosine Hydroxylasemolecular mechanism

references

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