Effect of composite salviae dropping pill on endothelin gene expression in circulating endothelial cells of patients with coronary heart disease

Peifang Feng; Nanping Qin; Yuguo Qin; Zhigang Chen; Jiuxian Feng

doi:10.1007/BF02947370

Your Location：

Home >

Browse articles >

Effect of composite salviae dropping pill on endothelin gene expression in circulating endothelial cells of patients with coronary heart disease

Updated：2021-08-27

- Effect of composite salviae dropping pill on endothelin gene expression in circulating endothelial cells of patients with coronary heart disease
- Chinese Journal of Integrative Medicine Vol. 6, Issue 4, Pages: 250-253(2000)
- Affiliations：
  
  1. The Affiliated Hospital of Zhejiang College of TCM,Hangzhou
  2. Molecular Biology Department, Shanghai Chest Hospital,China
- Author bio：
- Funds：
- DOI：10.1007/BF02947370
  CLC：
- Published：2000，
Scan for full text
Feng, P., Qin, N., Qin, Y. et al. Effect of composite salviae dropping pill on endothelin gene expression in circulating endothelial cells of patients with coronary heart disease., CJIM 6, 250–253 (2000). https://doi.org/10.1007/BF02947370

Peifang Feng, Nanping Qin, Yuguo Qin, et al. Effect of composite salviae dropping pill on endothelin gene expression in circulating endothelial cells of patients with coronary heart disease. [J]. Chinese Journal of Integrative Medicine 6(4):250-253(2000)
Feng, P., Qin, N., Qin, Y. et al. Effect of composite salviae dropping pill on endothelin gene expression in circulating endothelial cells of patients with coronary heart disease., CJIM 6, 250–253 (2000). https://doi.org/10.1007/BF02947370 DOI：

Peifang Feng, Nanping Qin, Yuguo Qin, et al. Effect of composite salviae dropping pill on endothelin gene expression in circulating endothelial cells of patients with coronary heart disease. [J]. Chinese Journal of Integrative Medicine 6(4):250-253(2000) DOI： 10.1007/BF02947370.

摘要

Objective: To explore the effect of composite Salviae droping pill(CSDP) on endothelin (ET-1) gene expression in circulating endothelial cells.Mothods: Seventy cases of stable angina pectoris were randomly divided into two groups

the CSDP group and the isosorbide dinitrate (ID) group. They were treated with CS-DP and ID respectively

their ET-1 gene expression in endothelial cells of peripheral circulation was measured before and after treatment by reverse transcriptase-polymerase chain reaction(RT-PCR) and compared between the two groups as well as with that of healthy subjects.Results: Electrophoresis banding of 546 bp cDNA procured from 59 cases of 70 patients was positive

while no positive banding was obtained from the healthy subjects. Six cases from the 29 patients treated with CSDP had their banding turned to negative

while in the ID group

no one turned to negative after treatment. And ET-1 PCR product (absorbed optic density) in the CSDP group was markedly lower than that in the ID group

P>0.05.Conclusion: DSP could directly inhibit ET-1 gene expression in endothelial cells of peripheral circulation.

Abstract

the CSDP group and the isosorbide dinitrate (ID) group. They were treated with CS-DP and ID respectively

while no positive banding was obtained from the healthy subjects. Six cases from the 29 patients treated with CSDP had their banding turned to negative

while in the ID group

no one turned to negative after treatment. And ET-1 PCR product (absorbed optic density) in the CSDP group was markedly lower than that in the ID group

P>0.05.Conclusion: DSP could directly inhibit ET-1 gene expression in endothelial cells of peripheral circulation.

关键词

endothelingene expressioncomposite Salviae dropping pillcirculating endothelial cell

Keywords

endothelingene expressioncomposite Salviae dropping pillcirculating endothelial cell

references

Yanagisawa M, Kurhara H, Kimura S, et al. A novel potent vasoconstrictive peptide produced by vascular endothelial cells. Nature 1988;332:411–415.

Kurihara H. Endothlin:A potent vasoconstrictor associated with coronary vasospasm. Life Sci 1989:44(25): 1937–1941.

CHEN JZ, LI ZM. Internal Medicine. 4th Edition. Beijing: The People’ s Health Publishing House, 1984: 274- 276.

Chomczynski P, Sacchi N. Single-step method of DNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162:156–159.

Pekonem F, Nyman T, Rutanen E. Differential expression of mRNA for endothelin-related proteins in human endometium myometrium and leiomyoma. Molecular and Celluar Endocrinology 1994; 103:165–169.

The Evaluation Standard for Coronary Angina and ECG. Selection for preventing and treating pulmonary heart diseases, coronary heart diseases and hypertension Symposium. Beijing:People’s Health Publishing House. 1979:82–84.

Zhou XM, Lu ZY, Jiang DY. Experimental study of Salviae miltiorrhiza on prevention of arterial restenosis after angioplasty. CJIM 1996; 16(8):480–482.

Zhang ZZ, Li YW. The pharmacologic function of Sanchi extract on myocardium. Acta Parmaceutica Sinica 1980; 15 (7):385–390.

Wang ZX, Chu XM. The effect of Sanqi Guanxingning on platelet aggregation and blood viscosity. Chinese Traditional and Herbal Drugs 1983;14(3):29–30.

Tang J, Tang CS, Yang J, et al. Endothelin —Basic Clinics. Beijing: The Union Publishing House of Beijing Medical University and Peking Union Medical University 1994:310–312.

FULL TEXT LINK

More>

Views

327

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Effects of Corocalm (Shuguan Capsule, 疏冠胶囊) on acute myocardial ischemia in anesthetized dogs

Effects of Qindan Capsule (芩丹胶囊) on blood pressure, endothelin, calcitonin gene-related peptide and angiotensin-II in spontaneous hypertensive rats

Effects of Erigeron Injection on vascular endothelium in patients after intracoronary stenting

Clinical observation on effect of Kaixin Capsule in treating 40 patients with diabetic myocardial ischemia

Related Author

No data

Related Institution

Department of Hematology, the Affiliated Hospital of Guiyang Medical College

Key Laboratory of Basic Pharmacology of Guizhou and Department of Pharmacology, Zunyi Medical College

Fundamental Medical Sciences Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences

Department of Radioisotope, Qilu Hospital Affiliated to Shandong University

Department of TCM, Qilu Hospital Affiliated to Shandong University

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰