Preliminary study on chinese drug- induced apoptosis of thyrocytes in graves’ disease

Jiajun Zhao; Ling Gao; Xinqi Liu; Xueping Liu

doi:10.1007/BF02950926

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Preliminary study on chinese drug- induced apoptosis of thyrocytes in graves’ disease

Updated：2021-08-27

- Preliminary study on chinese drug- induced apoptosis of thyrocytes in graves’ disease
- Chinese Journal of Integrative Medicine Vol. 6, Issue 3, Pages: 196-199(2000)
- Affiliations：
  
  Department of Endocrinology, Shandong Provincial Hospital,Jinan
- Author bio：
- Funds：
- DOI：10.1007/BF02950926
  CLC：
- Published：2000，
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Zhao, J., Gao, L., Liu, X. et al. Preliminary study on chinese drug- induced apoptosis of thyrocytes in graves’ disease., CJIM 6, 196–199 (2000). https://doi.org/10.1007/BF02950926

Jiajun Zhao, Ling Gao, Xinqi Liu, et al. Preliminary study on chinese drug- induced apoptosis of thyrocytes in graves’ disease. [J]. Chinese Journal of Integrative Medicine 6(3):196-199(2000)
Zhao, J., Gao, L., Liu, X. et al. Preliminary study on chinese drug- induced apoptosis of thyrocytes in graves’ disease., CJIM 6, 196–199 (2000). https://doi.org/10.1007/BF02950926 DOI：

Jiajun Zhao, Ling Gao, Xinqi Liu, et al. Preliminary study on chinese drug- induced apoptosis of thyrocytes in graves’ disease. [J]. Chinese Journal of Integrative Medicine 6(3):196-199(2000) DOI： 10.1007/BF02950926.

摘要

Objective: To investigate the cellular and molecular mechanisms of some Chinese drugs on apoptosis of thyrocytes in Graves’ disease.Methods: Two to ten weeks before and after using Chinese drugs on 13 Graves’ disease patients with anti-thyroid drugs

patients’ thyroid tissue was aspired with percutaneous puncturing needle. The effects of some Chinese drugs on thyrocytes was studied by applying cell morphology (stained with nuclear fast red

NFR)

flow cytometry (stained with propidium iodide

PI)

and terminal uridine deoxynucleotidyl end-labelling (TUNEL) technique (alkaline phosphatase and CBIP/NBT

NFR). The size of thyroid gland was measured by color Doppler ultrasonography.Results: After Chinese drug treatment

compared with single anti-thyroid drug group

the size of thyroid was significantly reduced (P<0.01). The apoptosis of thyrocytes can be observed by cell morphology such as chromatin condensation and nuclear fragmentation. The apoptosis rate is 18.66% ±20.01% (n=13) in anti-thyroid drugs plus Chinese drugs group

2.11% ±1.78% (n= 13) in single anti-thyroid drug group (P<0. 01). For up to 2–10 weeks

more TUNEL-positive cells were found after treatment with Chinese drugs than those treated with anti-thyroid drug alone.Conclusion: Some Chinese drugs when used in combination with anti-thyroid drugs in treating Graves’ disease could induce apoptosis of thyrocytes.

Abstract

patients’ thyroid tissue was aspired with percutaneous puncturing needle. The effects of some Chinese drugs on thyrocytes was studied by applying cell morphology (stained with nuclear fast red

NFR)

flow cytometry (stained with propidium iodide

PI)

and terminal uridine deoxynucleotidyl end-labelling (TUNEL) technique (alkaline phosphatase and CBIP/NBT

NFR). The size of thyroid gland was measured by color Doppler ultrasonography.Results: After Chinese drug treatment

compared with single anti-thyroid drug group

2.11% ±1.78% (n= 13) in single anti-thyroid drug group (P<0. 01). For up to 2–10 weeks

关键词

Graves’ diseasethyrocytesapoptosisChinese drugs

Keywords

Graves’ diseasethyrocytesapoptosisChinese drugs

references

Green DR, Cotter TG. Introduction: Apoptosis in the immune system. Semin Immunol 1992;4:399.

Watson RW, Rotstein OD, Nathens AB, et al. Thiol-mediated redox regulation neutrophil apoptosis. Surgery 1996; 120(2):150–158.

Watson AJ, Askew JN, Benson RS. Poly (adenosine diphosphate ribose) polymerase inhibition prevents necrosis induced by H202 but not apoptosis. Gastroenterology 1995; 109(2):472–482.

Tsujitani S, Shirai H, Tatebe S, et al. Apoptotic cell death and its relationship to carcinogenesis in colorectal carcinoma. Cancer 1997; 77(8): 1711–1716.

Takiya S, Tagaya T, Takahashi K, et al. Role of transforming growth factor βj on hepatic regeneration and apoptosis in liver diseases. J Clin Pathol 1995; 48 (12): 1093 -1097.

Gaffney EF, O’Neill AJ, Staunton MJ. In situ end-labelling, light microscopic assessment and ultrastructure of apoptosis in lung carcinoma. J Clin Pathol 1995; 48(11): 1017 -1021.

Kotani T, Aratake Y, Hirai K, et al. Apoptosis in thyroid tissue from patients with Hashimoto’s thyroiditis. Autoimmunity 1995;20(4):231–236.

Dremier S, Golstein J, Mosselmans R, et al. Apoptosis in dog thyroid cell. Biochem Biophys Res Commun 1994; 200 (1):52–58.

Branet F, Caron P, Camallieres M, et al. Bel-2 proto-onco-gene expression in neoplastic and non-neoplastic thyroid tissue. Bull Cancer Paris 1996;83(3): 213–217.

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Department of Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran

Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran

Department of Anesthesia, Chi-Mei Medical Center, Liouying, Tainan

Department of Nursing, Min-Hwei College of Health Care Management, Tainan

Division of Endocrinology and Metabolism, Kaohsiung Veteran General Hospital Tainan Branch, Tainan

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