Mechanism of the effect of guzhen recipe on regulating immune function in senile rats

Liu Ying; Zhao Weikang; Jin Guoqin; Ling Changquan

doi:10.1007/BF02950929

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Mechanism of the effect of guzhen recipe on regulating immune function in senile rats

Updated：2021-08-27

- Mechanism of the effect of guzhen recipe on regulating immune function in senile rats
- Chinese Journal of Integrative Medicine Vol. 6, Issue 3, Pages: 204-207(2000)
- Affiliations：
  
  1. Department of Biochemistry, Shanghai University of Traditional Chinese Medicine,China,200032
  2. Department of Traditional Chinese Medicine, Changhai Hospital of the Second Military University,China
- Author bio：
- Funds：
- DOI：10.1007/BF02950929
  CLC：
- Published：2000，
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Liu, Y., Zhao, W., Jin, G. et al. Mechanism of the effect of guzhen recipe on regulating immune function in senile rats., CJIM 6, 204–207 (2000). https://doi.org/10.1007/BF02950929

Liu Ying, Zhao Weikang, Jin Guoqin, et al. Mechanism of the effect of guzhen recipe on regulating immune function in senile rats. [J]. Chinese Journal of Integrative Medicine 6(3):204-207(2000)
Liu, Y., Zhao, W., Jin, G. et al. Mechanism of the effect of guzhen recipe on regulating immune function in senile rats., CJIM 6, 204–207 (2000). https://doi.org/10.1007/BF02950929 DOI：

Liu Ying, Zhao Weikang, Jin Guoqin, et al. Mechanism of the effect of guzhen recipe on regulating immune function in senile rats. [J]. Chinese Journal of Integrative Medicine 6(3):204-207(2000) DOI： 10.1007/BF02950929.

摘要

Objective: To explore the effect of Guzhen recipe

GZR) on mRNA expression of interleukin-2 (IL-2) and interleukin-6 (IL-6) in senile rats as well as the relationship between dehydroepi-androsterone (DHEA) and its sulfate (DHEAS) and the immune function regulation of GZR.Methods: Northern Blot and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to detect the mRNA expression of IL-2 and IL-6

meanwhile coating-antibody-counting method was used to detect DHEA(S) level in serum.Results: Compared with the young group

the splenocyte mRNA expression of IL-2 in senile rats was lowered and that of IL-6 was increased along with the decrease of serum DHEA(S) level. GZR

however

could improve the above indices in the senile rats.Conclusion: GZR might regulate the immune function of aged rats through increasing the serum level of DHEA(S).

Abstract

Objective: To explore the effect of Guzhen recipe

meanwhile coating-antibody-counting method was used to detect DHEA(S) level in serum.Results: Compared with the young group

the splenocyte mRNA expression of IL-2 in senile rats was lowered and that of IL-6 was increased along with the decrease of serum DHEA(S) level. GZR

however

could improve the above indices in the senile rats.Conclusion: GZR might regulate the immune function of aged rats through increasing the serum level of DHEA(S).

关键词

Guzhen recipeinterleukin-2 mRNAinterleukin-6 mRNAdehydroepiandrosterone (sulfate)

Keywords

Guzhen recipeinterleukin-2 mRNAinterleukin-6 mRNAdehydroepiandrosterone (sulfate)

references

Shanghai College of TCM. Basic knowledge of traditional Chinese medicine. 1st ed. Shanghai: Shanghai Science and Technology Press, 1978:14.

Zhang YZ, Yao SY, Zhao HJ, et al. Effects of Guzhen recipe on the immune function in senile rats. Academic Journal of Shanghai College of TCM 1990;4(1): 52–53.

Huang ZY, Zhao WK. Mechanism of the Effect of Guzhen Recipe on the transcription of IL-2 mRNA in immune suppressed mice. CJIM 1997; 17(suppl):231–234.

Herbert J. The age of DHEA. Lancet 1995; 345(8959): 1193–1194.

Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroforme extraction. Anal Biochem 1987; 162:156–159.

Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A laboratory manual. 2nd ed. Translated by JIN Dongyan, LI Mengfeng. Beijing: Science Press, 1992:362–372.

Oste C. Polymerase chain reaction. Biotechnique 1988;6: 162–167.

Murphy P G, Grondin J, Altares M et al. Induction of interleukin-6 in axotomized sensory neurons. J Neurosci 1995;15(7):5130–5138.

Weksler ME. Immune senescence and adrenal steroids: immune dysregulation and the action of dehydroepiandrosterone in old animal. Eur J Clin Pharmacol 1993; 45 (suppl):21–23.

Daynes RA. Alterai regulation of IL-6 production with normal aging. J Immunal 1993; 150(12): 5219–5230.

Spencer NF. Does DHEAS restore immune competence in aged animals through its capacity to function as a natural modulator of peroxisome activities? Ann N Y Acad Sci 1995:774:143–148.

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