FOLLOWUS
DNA Molecular profiling: a new approach to quality control of chinese drugs
- Chinese Journal of Integrative Medicine Vol. 6, Issue 1, Pages: 71-75(2000)
- Affiliations：
1. Institute of Chinese Materia Medica, China Academy of TCM,Beijing
2. Now are in the Toyama Medical and Pharmaceutical Univeristy,Japan
3. Toyama Medical and Pharmaceutical University,Japan
4. The Chinese University of Hong Kong, HKSAR
- Author bio：
- Funds：
DOI：10.1007/BF02973161
CLC：Published：2000，
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Cao, H., Liu, Y., Komatsu, K. et al. DNA Molecular profiling: a new approach to quality control of chinese drugs., CJIM 6, 71–75 (2000). https://doi.org/10.1007/BF02973161
Hui Cao, Yuping Liu, Komatsu Katsuko, et al. DNA Molecular profiling: a new approach to quality control of chinese drugs. [J]. Chinese Journal of Integrative Medicine 6(1):71-75(2000)
Cao, H., Liu, Y., Komatsu, K. et al. DNA Molecular profiling: a new approach to quality control of chinese drugs., CJIM 6, 71–75 (2000). https://doi.org/10.1007/BF02973161 DOI：
Hui Cao, Yuping Liu, Komatsu Katsuko, et al. DNA Molecular profiling: a new approach to quality control of chinese drugs. [J]. Chinese Journal of Integrative Medicine 6(1):71-75(2000) DOI： 10.1007/BF02973161.
摘要
DNA technology based molecular profiling provides the ultimate and definitive means of authentication for Chinese drugs. RFLP
RP-PCR and microsatellite-SSR examine the whole genome. With the appropriate primer
it is in principle possible to differentiate closely related species and even one individual from another. PCR-SR
PCR-RFLP
AFLP
SCAR
DALP and DNA sequencing examine a defined region on the genome and produce well-defined conclusions. Since different regions on the genome evolve to different extents
by choosing the target DNA carefully
organisms of different systematic levels can be compared. Of these molecular techniques mentioned above
genomic fingerprints and DNA sequences are equally successful in identification of Chinese drugs. Although RFLP
RP-PCR methods do not require prior to genetic background of the genome and can detect DNA polymorphism
the reliability of these fingerprint results can be greatly influenced by experimental conditions. DNA sequencing method focuses on a defined locus on the genome and may provide a definitive means for authentication of Chinese drugs. We
therefore
conclude that it is essential to combine genomic fingerprinting with DNA sequencing analysis in order to increase the accuracy of quality control.
Abstract
DNA technology based molecular profiling provides the ultimate and definitive means of authentication for Chinese drugs. RFLP
RP-PCR and microsatellite-SSR examine the whole genome. With the appropriate primer
it is in principle possible to differentiate closely related species and even one individual from another. PCR-SR
PCR-RFLP
AFLP
SCAR
DALP and DNA sequencing examine a defined region on the genome and produce well-defined conclusions. Since different regions on the genome evolve to different extents
by choosing the target DNA carefully
organisms of different systematic levels can be compared. Of these molecular techniques mentioned above
genomic fingerprints and DNA sequences are equally successful in identification of Chinese drugs. Although RFLP
RP-PCR methods do not require prior to genetic background of the genome and can detect DNA polymorphism
the reliability of these fingerprint results can be greatly influenced by experimental conditions. DNA sequencing method focuses on a defined locus on the genome and may provide a definitive means for authentication of Chinese drugs. We
therefore
conclude that it is essential to combine genomic fingerprinting with DNA sequencing analysis in order to increase the accuracy of quality control.
关键词
Internal Transcribe SpacerRestriction Fragment Length PolymorphismAmplify Fragment Length PolymorphismPanax GinsengAmerican Ginseng
Keywords
Internal Transcribe SpacerRestriction Fragment Length PolymorphismAmplify Fragment Length PolymorphismPanax GinsengAmerican Ginseng
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