Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride

Cao Hong-xin; Sun Hui; Jiang Xin-gang; Lu Hai-tao; Zhang Guang-mei; Wang Xi-jun; Sun Wen-jun; Wu Ze-ming; Wang Ping; Liu Lian; Zhou Jue

doi:10.1007/s11655-009-0204-y

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Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride

Updated：2021-08-27

- Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride
- Chinese Journal of Integrative Medicine Vol. 15, Issue 3, Pages: 204-209(2009)
- Affiliations：
  
  1. Department of Pharmacognosy, Heilongjiang University of Chinese Medicine,Harbin,China
  2. China Academy of Chinese Medical Sciences,Beijing,China
  3. First Hospital, Harbin Medical University,Harbin,China
- Author bio：
- Funds：
  
  Supported by the Major State Basic Research Development Program of China (973 Program, No. 2005CB523406)
- DOI：10.1007/s11655-009-0204-y
  CLC：
- Published：2009，
  
  Published Online：2 July 2009，
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Hong-xin, C., Hui, S., Xin-gang, J. et al. Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride., Chin. J. Integr. Med. 15, 204 (2009). https://doi.org/10.1007/s11655-009-0204-y

Cao Hong-xin, Sun Hui, Jiang Xin-gang, et al. Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride. [J]. Chinese Journal of Integrative Medicine 15(3):204-209(2009)
Hong-xin, C., Hui, S., Xin-gang, J. et al. Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride., Chin. J. Integr. Med. 15, 204 (2009). https://doi.org/10.1007/s11655-009-0204-y DOI：

Cao Hong-xin, Sun Hui, Jiang Xin-gang, et al. Comparative study on the protective effects of Yinchenhao Decoction (茵陈蒿汤) against liver injury induced by α-naphthylisothiocyanate and carbon tetrachloride. [J]. Chinese Journal of Integrative Medicine 15(3):204-209(2009) DOI： 10.1007/s11655-009-0204-y.

摘要

To optimize the animal model of liver injury that can properly represent the pathological characteristics of dampness-heat jaundice syndrome of traditional Chinese medicine. The liver injury in the model rat was induced by α-naphthylisothiocyanate (ANIT) and carbon tetrachloride (CCl4 ) respectively

and the effects of Yinchenhao Decoction (茵陈蒿汤

YCHD)

a proved effective Chinese medical formula for treating the dampness-heat jaundice syndrome in clinic

on the two liver injury models were evaluated by analyzing the serum level of alanine aminotransferase (ALT)

asparate aminotransferase (AST)

alkaline phosphatase (ALP)

malondialchehyche (MDA)

total bilirubin (T-BIL)

superoxide dismutase (SOD)

glutathione peroxidase (GSH-PX) as well as the ratio of liver weight to body weight. The experimental data were analyzed by principal component analytical method of pattern recognition. The ratio of liver weight to body weight was significantly elevated in the ANIT and CCl4 groups when compared with that in the normal control (P<0.01). The contents of ALT and T-BIL were significantly higher in the ANIT group than in the normal control (P<0.05

P<0.01)

and the levels of AST

ALT and ALP were significantly elevated in CCl4 group relative to those in the normal control P<0.01). In the YCHD group

the increase in AST

ALT and ALP levels was significantly reduced (P<0.05

P<0.01)

but with no significant increase in serum T-BIL. In the CCl4 intoxicated group

the MDA content was significantly increased and SOD

GSH-PX activities decreased significantly compared with those in the normal control group

respectively (P<0.01). The increase in MDA induced by CCl4 was significantly reduced by YCHD P<0.05). YCHD showed significant effects on preventing liver injury progression induced by CCl4

and the closest or most suitable animal model for damp-heat jaundice syndrome may be the one induced by CCl4.

Abstract

and the effects of Yinchenhao Decoction (茵陈蒿汤

YCHD)

a proved effective Chinese medical formula for treating the dampness-heat jaundice syndrome in clinic

on the two liver injury models were evaluated by analyzing the serum level of alanine aminotransferase (ALT)

asparate aminotransferase (AST)

alkaline phosphatase (ALP)

malondialchehyche (MDA)

total bilirubin (T-BIL)

superoxide dismutase (SOD)

P<0.01)

and the levels of AST

ALT and ALP were significantly elevated in CCl4 group relative to those in the normal control P<0.01). In the YCHD group

the increase in AST

ALT and ALP levels was significantly reduced (P<0.05

P<0.01)

but with no significant increase in serum T-BIL. In the CCl4 intoxicated group

the MDA content was significantly increased and SOD

GSH-PX activities decreased significantly compared with those in the normal control group

respectively (P<0.01). The increase in MDA induced by CCl4 was significantly reduced by YCHD P<0.05). YCHD showed significant effects on preventing liver injury progression induced by CCl4

and the closest or most suitable animal model for damp-heat jaundice syndrome may be the one induced by CCl4.

关键词

Yinchenhao Decoctionα-naphthylisothiocyanatecarbon tetrachlorideliver injurydampness-heat jaundice syndrome

Keywords

Yinchenhao Decoctionα-naphthylisothiocyanatecarbon tetrachlorideliver injurydampness-heat jaundice syndrome

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Animal Laboratory Center, Hospital of Chinese People’s Liberation Army

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