Anti-Inflammatory mechanism of total glycosides of Acanthopanax Giraldii

Fang Yuan; Jie Chen; Guo-min Xu; Jia-jia Zheng; Qi-cai Long

doi:10.1007/s11655-009-0210-0

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Anti-Inflammatory mechanism of total glycosides of Acanthopanax Giraldii

Updated：2021-08-27

- Anti-Inflammatory mechanism of total glycosides of Acanthopanax Giraldii
- Chinese Journal of Integrative Medicine Vol. 15, Issue 3, Pages: 210-215(2009)
- Affiliations：
  
  1. Institute of Clinical Pharmacology, School of Pharmaceutical Sciences, Sun Yat-sen University,Guangzhou,China
  2. Department of Pharmacy, the First Affiliated Hospital, Sun Yat-sen University,Guangzhou,China
  3. Shenzhen Buji People’s Hospital,Guangdong,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-009-0210-0
  CLC：
- Published：2009，
  
  Published Online：2 July 2009，
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Yuan, F., Chen, J., Xu, Gm. et al. Anti-Inflammatory mechanism of total glycosides of Acanthopanax Giraldii., Chin. J. Integr. Med. 15, 210–215 (2009). https://doi.org/10.1007/s11655-009-0210-0

Fang Yuan, Jie Chen, Guo-min Xu, et al. Anti-Inflammatory mechanism of total glycosides of Acanthopanax Giraldii. [J]. Chinese Journal of Integrative Medicine 15(3):210-215(2009)
Yuan, F., Chen, J., Xu, Gm. et al. Anti-Inflammatory mechanism of total glycosides of Acanthopanax Giraldii., Chin. J. Integr. Med. 15, 210–215 (2009). https://doi.org/10.1007/s11655-009-0210-0 DOI：

Fang Yuan, Jie Chen, Guo-min Xu, et al. Anti-Inflammatory mechanism of total glycosides of Acanthopanax Giraldii. [J]. Chinese Journal of Integrative Medicine 15(3):210-215(2009) DOI： 10.1007/s11655-009-0210-0.

摘要

To study the anti-inflammatory mechanisms of total glycosides of Acanthopanax Giraldii (TGA). The changes of prostaglandin E2(PGE2)

tumor necrosis factor (TNF-α)

nitric oxide (NO)

and expressions of COX-1 mRNA and COX-2 mRNA in BALB/c mouse macrophages were observed by the radioimmunoassay

ELISA and nitric acid reduction and RT-PCR in the presence or absence of TGA. (1) TGA could significantly decrease the production of PGE2and NO in mouse peritoneal macrophages. The inhibitory rate to LPS-induced PGE2production was 87% (TGA 100 mg/L

P<0.05

vs. LPS) and 62% (TGA 20 mg/L

P<0.05

vs. LPS)

respectively. The inhibitory rate of NO production in mouse peritoneal macrophages was 49% (TGA 100 mg/L

P<0.05

vs. LPS) and 21% (TGA 20 mg/L

P<0.05 vs. LPS)

respectively. TGA could not inhibit LPS-induced TNF-α production in mouse peritoneal macrophages. (2) TGA also inhibited the expression of COX-1 and COX-2 mRNA in RAW264.7 cells. The inhibitory rate of TGA to COX-1 mRNA was 22% (TGA 100 mg/L

P<0.05

vs. blank). The inhibitory rate of TGA to COX-2 mRNA was 55% (TGA 20 mg/L

P<0.05

vs. LPS) and 100% (TGA 100 mg/L

P<0.01 vs. LPS)

respectively. The anti-inflammatory mechanisms of TGA for inhibiting the production of NO and PGE2are through inhibiting COX-2 mRNA expression without TNF-α changes.

Abstract

To study the anti-inflammatory mechanisms of total glycosides of Acanthopanax Giraldii (TGA). The changes of prostaglandin E2(PGE2)

tumor necrosis factor (TNF-α)

nitric oxide (NO)

and expressions of COX-1 mRNA and COX-2 mRNA in BALB/c mouse macrophages were observed by the radioimmunoassay

P<0.05

vs. LPS) and 62% (TGA 20 mg/L

P<0.05

vs. LPS)

respectively. The inhibitory rate of NO production in mouse peritoneal macrophages was 49% (TGA 100 mg/L

P<0.05

vs. LPS) and 21% (TGA 20 mg/L

P<0.05 vs. LPS)

P<0.05

vs. blank). The inhibitory rate of TGA to COX-2 mRNA was 55% (TGA 20 mg/L

P<0.05

vs. LPS) and 100% (TGA 100 mg/L

P<0.01 vs. LPS)

respectively. The anti-inflammatory mechanisms of TGA for inhibiting the production of NO and PGE2are through inhibiting COX-2 mRNA expression without TNF-α changes.

关键词

total glycosides of Acanthopanax Giraldiiprostaglandin E2Nitric Oxidetumor necrosis factorCOX mRNA

Keywords

total glycosides of Acanthopanax Giraldiiprostaglandin E2Nitric Oxidetumor necrosis factorCOX mRNA

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