Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia

Yuan-peng Huang; Jian Du; Zhen-feng Hong; Zhi-qing Chen; Jin-fa Wu; Jin-yan Zhao

doi:10.1007/s11655-009-0289-3

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Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia

Updated：2021-08-27

- Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia
- Chinese Journal of Integrative Medicine Vol. 15, Issue 4, Pages: 289-292(2009)
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, Zhongshan Hospital, Xiamen University,Fujian,China
  2. Academy of Integrative Medicine, Fujian College of Traditional Chinese Medicine,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the Foundation of Traditional Chinese Medicine of Fujian Province (No. wzy0616), the Foundation of Key Projects from Science and Technology Department of Fujian Province (No. 2008Y0049), and the Foundation of Science and Technology Department of Xiamen (No. 3502Z20084014)
- DOI：10.1007/s11655-009-0289-3
  CLC：
- Published：2009，
  
  Published Online：18 August 2009，
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Huang, Yp., Du, J., Hong, Zf. et al. Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia., Chin. J. Integr. Med. 15, 289–292 (2009). https://doi.org/10.1007/s11655-009-0289-3

Yuan-peng Huang, Jian Du, Zhen-feng Hong, et al. Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia. [J]. Chinese Journal of Integrative Medicine 15(4):289-292(2009)
Huang, Yp., Du, J., Hong, Zf. et al. Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia., Chin. J. Integr. Med. 15, 289–292 (2009). https://doi.org/10.1007/s11655-009-0289-3 DOI：

Yuan-peng Huang, Jian Du, Zhen-feng Hong, et al. Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia. [J]. Chinese Journal of Integrative Medicine 15(4):289-292(2009) DOI： 10.1007/s11655-009-0289-3.

摘要

To investigate the effects of Kangquan Recipe (康泉方

KQR) on sex steroids and cell proliferation in an experimental benign prostatic hyperplasia (BPH) model in rats. Seventy-two SD rats were randomly divided into six groups: the normal group

the model group

the finasteride group

and the low-

middle-

and high-dose KQR groups

12 in each group. Except those in the normal group

the rats were injected with testosterone after castration for the establishment of BPH model and then given respectively with normal saline

finasteride

and low-

middle-

and high-dose of KQR for 30 days. The levels of plasma testosterone (T) and estradiol (E2) were determined by enzyme-linked immunosorbent assay (ELISA)

and the mRNA expression ) of proliferating cell nuclear antigen (PCNA) in prostate tissue was detected by reverse transcription-polymerase chain reaction (RT-PCR) after administration. Compared with the model group

the prostate weight

the plasma T

and the mRNA expression of PCNA were significantly lower

and the plasma E2 and the ratio of E2/T were higher in the three KQR groups (P<0.05 or P<0.01). There was no significant difference in the prostate weight

plasma T and E2

and ratio of E2/T among the finasteride group and the three KQR groups (P>0.05). The mRNA expressions of PCNA were significantly higher in the middle- and low-dose of KQR groups than those in the finasteride group (P<0.05). KQR shows multitarget effects on experimental BPH rats

and the mechanism might be related with regulating the balance of plasma T and E2 and decreasing the PCNAmRNA expression in prostate tissue to restrain cell proliferation in a dose-dependent manner.

Abstract

To investigate the effects of Kangquan Recipe (康泉方

KQR) on sex steroids and cell proliferation in an experimental benign prostatic hyperplasia (BPH) model in rats. Seventy-two SD rats were randomly divided into six groups: the normal group

the model group

the finasteride group

and the low-

middle-

and high-dose KQR groups

12 in each group. Except those in the normal group

the rats were injected with testosterone after castration for the establishment of BPH model and then given respectively with normal saline

finasteride

and low-

middle-

and high-dose of KQR for 30 days. The levels of plasma testosterone (T) and estradiol (E2) were determined by enzyme-linked immunosorbent assay (ELISA)

the prostate weight

the plasma T

and the mRNA expression of PCNA were significantly lower

and the plasma E2 and the ratio of E2/T were higher in the three KQR groups (P<0.05 or P<0.01). There was no significant difference in the prostate weight

plasma T and E2

and the mechanism might be related with regulating the balance of plasma T and E2 and decreasing the PCNAmRNA expression in prostate tissue to restrain cell proliferation in a dose-dependent manner.

关键词

Kangquan Recipebenign prostatic hyperplasiaTestosteroneestradiolcell proliferation

Keywords

Kangquan Recipebenign prostatic hyperplasiaTestosteroneestradiolcell proliferation

references

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Ministry of Health, P.R. China. Guidance on principle of preclinical research on new drugs. Beijing: People’s Medical Publishing House; 1993:101–103.

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