Protective effect of effective composite of Chinese medicine prescription Naodesheng (脑得生) against focal cerebral ischemia in rats

Li Zhang; Xin-rui Cheng; Ruo-yun Chen; Xiao-ming Zhu; Guan-hua Du

doi:10.1007/s11655-009-0377-4

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Protective effect of effective composite of Chinese medicine prescription Naodesheng (脑得生) against focal cerebral ischemia in rats

Updated：2021-08-27

- Protective effect of effective composite of Chinese medicine prescription Naodesheng (脑得生) against focal cerebral ischemia in rats
- Chinese Journal of Integrative Medicine Vol. 15, Issue 5, Pages: 377-383(2009)
- Affiliations：
  
  1. National Center for Pharmaceutical Screening, Institute of Materia Medica, Chinese Academy of Medical Science & Peking Union Medical College,Beijing,China
  2. Department of Pharmacology, Institute of Basic Medical Science, Chinese Academy of Medical Science & Peking Union Medical College,Beijing,China
- Author bio：
- Funds：
  
  Supported by Chinese Herbs Special Project of State Administration of Traditional Chinese Medicine (No. 02-03-ZP08);National High Technology Research and Development Program of China, the Fund of Research and Application of Screening Technology Platform of Innovative Drug (863 Program, No. 2004AA2Z3782)
- DOI：10.1007/s11655-009-0377-4
  CLC：
- Published：2009，
  
  Published Online：3 October 2009，
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Zhang, L., Cheng, Xr., Chen, Ry. et al. Protective effect of effective composite of Chinese medicine prescription Naodesheng (脑得生) against focal cerebral ischemia in rats., Chin. J. Integr. Med. 15, 377–383 (2009). https://doi.org/10.1007/s11655-009-0377-4

Li Zhang, Xin-rui Cheng, Ruo-yun Chen, et al. Protective effect of effective composite of Chinese medicine prescription Naodesheng (脑得生) against focal cerebral ischemia in rats. [J]. Chinese Journal of Integrative Medicine 15(5):377-383(2009)
Zhang, L., Cheng, Xr., Chen, Ry. et al. Protective effect of effective composite of Chinese medicine prescription Naodesheng (脑得生) against focal cerebral ischemia in rats., Chin. J. Integr. Med. 15, 377–383 (2009). https://doi.org/10.1007/s11655-009-0377-4 DOI：

Li Zhang, Xin-rui Cheng, Ruo-yun Chen, et al. Protective effect of effective composite of Chinese medicine prescription Naodesheng (脑得生) against focal cerebral ischemia in rats. [J]. Chinese Journal of Integrative Medicine 15(5):377-383(2009) DOI： 10.1007/s11655-009-0377-4.

摘要

To study the effects and possible mechanisms of effective composite of Naodesheng (脑得生

NDS) on permanent cerebral ischemia-induced injury in rats. Male Sprague-Dawley rats: with middle cerebral artery occlusion (MCAO) were established with the modified suture method

and they were randomly divided into the following groups: the sham-operated group

the model group

the Nimodipine group (0.012 g/kg)

the NDS group (1.075 g/kg)

the total extracts group (0.23 g/kg)

the high-dose NEC group (0.07 g/kg)

the middle-dose NEC group (0.02 g/kg)

and the low-dose NEC group (0.007 g/kg). The aforesaid medicines were administered at the 2nd

4th

and 24th h after focal cerebral ischemia

and the infarction size and water content in the brain were evaluated at the 26th h after MCAO. Then

after oral administration once daily for 7 successive days

the changes in the degree of neurological deficit

oxidative stress

and apoptosis were measured on the 7th day. NEC could significantly reduce the infarction size after focal cerebral ischemia

and slightly relieve water content in the brain

significantly alleviate neurological function impairment

increase the levels of superoxide dismutase (SOD) and adenosine triphosphate enzyme (ATPase) activity

and decrease the content of malondialdehyde (MDA). NEC could also extenuate Bax and caspase-3 expression in the hippocampus tissue of the ischemic region. As compared with the three NEC treated groups

the high-dose NEC showed better efficacy. NEC could significantly reduce brain injury induced by ischemia;: its mechanism was closely associated with hindering oxidative stress and apoptosis. The effective composite-compositeguided methodology is a feasible tool to improve the neuro-protective properties of the Chinese medicine guided prescription NDS against focal cerebral ischemia in rats.

Abstract

To study the effects and possible mechanisms of effective composite of Naodesheng (脑得生

NDS) on permanent cerebral ischemia-induced injury in rats. Male Sprague-Dawley rats: with middle cerebral artery occlusion (MCAO) were established with the modified suture method

and they were randomly divided into the following groups: the sham-operated group

the model group

the Nimodipine group (0.012 g/kg)

the NDS group (1.075 g/kg)

the total extracts group (0.23 g/kg)

the high-dose NEC group (0.07 g/kg)

the middle-dose NEC group (0.02 g/kg)

and the low-dose NEC group (0.007 g/kg). The aforesaid medicines were administered at the 2nd

4th

and 24th h after focal cerebral ischemia

and the infarction size and water content in the brain were evaluated at the 26th h after MCAO. Then

after oral administration once daily for 7 successive days

the changes in the degree of neurological deficit

oxidative stress

and apoptosis were measured on the 7th day. NEC could significantly reduce the infarction size after focal cerebral ischemia

and slightly relieve water content in the brain

significantly alleviate neurological function impairment

increase the levels of superoxide dismutase (SOD) and adenosine triphosphate enzyme (ATPase) activity

关键词

effective compositeNaodeshengischemianeuro-protectionapoptosis

Keywords

effective compositeNaodeshengischemianeuro-protectionapoptosis

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