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Effects of Danggui Buxue Decoction (当归补血汤) on lipid peroxidation and MMP-2/9 activities of fibrotic liver in rats
- Chinese Journal of Integrative Medicine Vol. 15, Issue 6, Pages: 435-441(2009)
- Affiliations：
1. Shuguang Hospital, Liver Disease Institute, Shanghai University of Traditional Chinese Medicine,Shanghai,China
2. The Experimental Center of Science and Technology, Shanghai University of Traditional Chinese Medicine,Shanghai,China
3. Shanghai Traditional Chinese Medicine Internal Medicine E-Institute, Key Lab of Liver & Kidney Diseases, Shanghai University of Traditional Chinese Medicine, Minister of Education,Shanghai,China
- Author bio：
- Funds：Supported by the National Natural Science Foundation of China (No. 30772869), Major State Basic Research Development of China (973 Program, No, 2006CB504801) Shanghai Subject Chief Scientist (No. 08XD1404100), E-institute of Shanghai Municipal Education Commission (No. 03008), Innovative Research Team in Shanghai Municipal Education Commission
DOI：10.1007/s11655-009-0435-y
CLC：Published：2009，
Published Online：18 January 2010，
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Chen, Y., Chen, Q., Lu, J. et al. Effects of Danggui Buxue Decoction (当归补血汤) on lipid peroxidation and MMP-2/9 activities of fibrotic liver in rats., Chin. J. Integr. Med. 15, 435–441 (2009). https://doi.org/10.1007/s11655-009-0435-y
Yuan Chen, Qian Chen, Jing Lu, et al. Effects of Danggui Buxue Decoction (当归补血汤) on lipid peroxidation and MMP-2/9 activities of fibrotic liver in rats. [J]. Chinese Journal of Integrative Medicine 15(6):435-441(2009)
Chen, Y., Chen, Q., Lu, J. et al. Effects of Danggui Buxue Decoction (当归补血汤) on lipid peroxidation and MMP-2/9 activities of fibrotic liver in rats., Chin. J. Integr. Med. 15, 435–441 (2009). https://doi.org/10.1007/s11655-009-0435-y DOI：
Yuan Chen, Qian Chen, Jing Lu, et al. Effects of Danggui Buxue Decoction (当归补血汤) on lipid peroxidation and MMP-2/9 activities of fibrotic liver in rats. [J]. Chinese Journal of Integrative Medicine 15(6):435-441(2009) DOI： 10.1007/s11655-009-0435-y.
摘要
To explore the mechanism of Danggui Buxue Decoction (当归补血汤
DBD) on the liver fifibrosis related to hepatic lipid peroxidation and matrix metalloproteinases (MMP) −2/9 activities. The liver fifibrosis in 28 rats was induced by an injection of carbon tetrachloride (CCl4) and fed with high lipid and low protein diet for 6 weeks
the model rats were randomly divided into the model group and DBD treated group
14 in each group
and another 10 rats as the normal group were observed as well. Rats in the DBD group were administered with DBD at the dose of 6 g/kg body weight for 6 weeks since CCl4 intoxication. The hepatic inflammation and fibrosis were examined with HE and Sirius red stain. The liver function including serum alanine aminotransamine (ALT)
aspartate transamine (AST)
albumin (Alb) and total bilirubin (TBIL)
liver triglyceride (TG) and malondialdehyde (MDA) contents
superoxide dismutase (SOD) activity were assayed. Hepatic hydroxyproline (Hyp) content was detected with Jamall’s method. The α-SMA expression was analyzed by immunohistochemistry and the Western blot. Liver MMP-2 mRNA was analyzed with Real-time PCR
and MMP-2/9 activities were measured with gelatin zymography and in situ zymography. Compared with the normal group
the levels of ALT
AST and TBIL
the content of Hyp
TG and MDA were remarkably increased
the Alb content and SOD activity were signifificantly decreased in the model group (P<0.05)
and higher levels of MMP-2 mRNA and MMP-2/9 activities (P<0.01)
the hepatic fatty degeneration and collagen accumulation and fifibrosis at liver were observed. Compared with the model control
DBD group showed slighter hepatic fatty degeneration and collagen deposition
and had lower levels of ALT
AST and TBIL activities
lower contents of MDA
TG and Hyp
but higher SOD level and Alb content (P<0.05)
and DBD also down-regulated MMP-2 mRNA expression and decreased MMP-2/9 activities in the fifibrotic livers (P<0.01). The action of DBD against liver fibrosis is related to prevent lipid peroxidation and inhibit MMP-2/9 activities in the fibrotic livers.
Abstract
To explore the mechanism of Danggui Buxue Decoction (当归补血汤
DBD) on the liver fifibrosis related to hepatic lipid peroxidation and matrix metalloproteinases (MMP) −2/9 activities. The liver fifibrosis in 28 rats was induced by an injection of carbon tetrachloride (CCl4) and fed with high lipid and low protein diet for 6 weeks
the model rats were randomly divided into the model group and DBD treated group
14 in each group
and another 10 rats as the normal group were observed as well. Rats in the DBD group were administered with DBD at the dose of 6 g/kg body weight for 6 weeks since CCl4 intoxication. The hepatic inflammation and fibrosis were examined with HE and Sirius red stain. The liver function including serum alanine aminotransamine (ALT)
aspartate transamine (AST)
albumin (Alb) and total bilirubin (TBIL)
liver triglyceride (TG) and malondialdehyde (MDA) contents
superoxide dismutase (SOD) activity were assayed. Hepatic hydroxyproline (Hyp) content was detected with Jamall’s method. The α-SMA expression was analyzed by immunohistochemistry and the Western blot. Liver MMP-2 mRNA was analyzed with Real-time PCR
and MMP-2/9 activities were measured with gelatin zymography and in situ zymography. Compared with the normal group
the levels of ALT
AST and TBIL
the content of Hyp
TG and MDA were remarkably increased
the Alb content and SOD activity were signifificantly decreased in the model group (P<0.05)
and higher levels of MMP-2 mRNA and MMP-2/9 activities (P<0.01)
the hepatic fatty degeneration and collagen accumulation and fifibrosis at liver were observed. Compared with the model control
DBD group showed slighter hepatic fatty degeneration and collagen deposition
and had lower levels of ALT
AST and TBIL activities
lower contents of MDA
TG and Hyp
but higher SOD level and Alb content (P<0.05)
and DBD also down-regulated MMP-2 mRNA expression and decreased MMP-2/9 activities in the fifibrotic livers (P<0.01). The action of DBD against liver fibrosis is related to prevent lipid peroxidation and inhibit MMP-2/9 activities in the fibrotic livers.
关键词
Danggui Buxue Decoctionliver fifibrosishepatic lipid peroxidationmatrix metalloproteinases-2/9
Keywords
Danggui Buxue Decoctionliver fifibrosishepatic lipid peroxidationmatrix metalloproteinases-2/9
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