Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner

Hong Nie; Lan-zhen Meng; Jiu-yao Zhou; Xiao-feng Fan; Yong Luo-; Guang-wen Zhang

doi:10.1007/s11655-009-0442-z

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Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner

Updated：2021-08-27

- Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner
- Chinese Journal of Integrative Medicine Vol. 15, Issue 6, Pages: 442-447(2009)
- Affiliations：
  
  1. College of Pharmacy, Jinan University,Guangzhou,China
  2. Guangzhou University of Chinese Medicine,Guangzhou,China
  3. Research Center for Analytical Sciences of Northeastern University,Shenyang,China
  4. College of Science and Engineering, Jinan University,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30400596), Jinan University Natural Science Foundation (No. 51204017), and 211 Project of Jinan University
- DOI：10.1007/s11655-009-0442-z
  CLC：
- Published：2009，
  
  Published Online：18 January 2010，
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Nie, H., Meng, Lz., Zhou, Jy. et al. Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner., Chin. J. Integr. Med. 15, 442–447 (2009). https://doi.org/10.1007/s11655-009-0442-z

Hong Nie, Lan-zhen Meng, Jiu-yao Zhou, et al. Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner. [J]. Chinese Journal of Integrative Medicine 15(6):442-447(2009)
Nie, H., Meng, Lz., Zhou, Jy. et al. Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner., Chin. J. Integr. Med. 15, 442–447 (2009). https://doi.org/10.1007/s11655-009-0442-z DOI：

Hong Nie, Lan-zhen Meng, Jiu-yao Zhou, et al. Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner. [J]. Chinese Journal of Integrative Medicine 15(6):442-447(2009) DOI： 10.1007/s11655-009-0442-z.

摘要

To demonstrate the vasodilatation activity of the coumarin-containing Angelica dahurica var. formosana and to further analyze active components in the herb extracts. (1) The vasodilatation effects induced by different extracts (cyclohexane

ethyl acetate

acetone

methanol

95 % ethanol and water) of Angelica dahurica var. formosana on mouse thoracic aorta pre-contracted with phenylephrine were investigated. (2) The amount of imperatorin and isoimperatorin in each extract was measured by high-performance liquid chromatography. (3) The vasodilatation effects of imperatorin and isoimperatorin on mouse thoracic aorta were compared using the same in vitro method. (4) The vasodilatation mechanism of imperatorin in the mouse thoracic aorta pre-contracted with phenylephrine was studied using the methods of denuded endothelium

NG-nitro-L-arginine methylester (L-NAME

a nitric oxide synthase inhibitor)

and propranolol. (1) The cyclohexane and ethyl acetate extracts of Angelica dahurica var. formosana decreased the maximal response of phenylephrine-induced mouse thoracic aorta contraction dose-dependently

with 50% inhibiting concentration (IC50) values of 35.3±12.4 mg/L and 40.5±12.0 mg/L

respectively. The vasodilatation effect of imperatorin and isoimperatorin was dose-dependent. (2) The cyclohexane extract

showing the strongest vasodilatation effect

possessed the highest contents of imperatorin (4.09%) and isoimperatorin (0.27%

w/w). There was a correlation between the vasodilatation activity and the contents of imperatorin and isoimperatorin in the extracts. (3) The vasodilatation effect of imperatorin was about 4-fold stronger than that of isoimperatorin. (4) The vasodilatation effect of imperatorin was signifificantly attenuated to 24.88%±4.06% in the denuded endothelium group compared with the intact endothelium group. And 1 mmol/L L-NAME reduced the imperatorin-induced vasorelaxation by 32.18 %±11.29 %. The principal effective component of Angelica dahurica var. Formosana was found to be imperatorin. Imperatorin-induced vasodilatation is at least partially regulated by nitric oxide

and has no correlation to β-receptor.

Abstract

ethyl acetate

acetone

methanol

NG-nitro-L-arginine methylester (L-NAME

a nitric oxide synthase inhibitor)

with 50% inhibiting concentration (IC50) values of 35.3±12.4 mg/L and 40.5±12.0 mg/L

respectively. The vasodilatation effect of imperatorin and isoimperatorin was dose-dependent. (2) The cyclohexane extract

showing the strongest vasodilatation effect

possessed the highest contents of imperatorin (4.09%) and isoimperatorin (0.27%

and has no correlation to β-receptor.

关键词

Angelica dahurica var. formosanathoracic aortavasodilatationimperatorinisoimperatorinhigh-performance liquid chromatography

Keywords

Angelica dahurica var. formosanathoracic aortavasodilatationimperatorinisoimperatorinhigh-performance liquid chromatography

references

Ban HS, Lim SS, Suzuki K, SH, Lee S, Lee YS, et al. Inhibitory effects of furanocoumarins isolated from the roots of Angelica dahurica on prostaglandin E2 production. Planta Med 2003;69:408–412.

Song DK, Kim JY, Li G, Lee KS, Seo CS, Yan JJ, et al. Agents protecting against sepsis from the roots of Angelica dahurica. Biol Pharm Bull 2005;28:380–382.

Kim YK, Kim YS, Ryu SY. Antiproliferative effect of furanocoumarins from the root of Angelica dahurica on cultured human tumor cell lines. Phytother Res 2007;21:288–290.

Ketai W, Huitao L, Xingguo C, Yunkun Z, Zhide H. Identification and determination of active components in Angelica dahurica Benth and its medicinal preparation by capillary electrophoresis. Talanta 2001;54:753–761.

Liu RM, Li AF, Sun AL. Preparative isolation and purification of coumarins from Angelica dahurica (Fisch. ex Hoffm) Benth. et Hook. f (Chinese traditional medicinal herb) by high-speed counter-current chromatography. J Chromatogr A 2004;1052:223–227.

Wei Y, Ito Y. Preparative isolation of imperatorin, oxypeucedanin and isoimperatorin from traditional Chinese herb “bai zhi” Angelica dahurica (Fisch. ex Hoffm) Benth. et Hook using multidimensional high-speed counter-current chromatography. J Chromatogr A 2006;1115:112–117.

Gilani AH, Shaheen E, Saeed SA, Bibi S, Irfanullah, Sadiq M, et al. Hypotensive action of coumarin glycosides from Daucus carota. Phytomed 2000;7:423–426.

Campos-Toimil M, Orallo F, Santana L, Uriarte E. Synthesis and vasorelaxant activity of new coumarin and furocoumarin derivatives. Bioorg Med Chem Lett 2002;12:783–786.

Shen B, Ye CL, Ye KH, Liu JJ. Mechanism underlying enhanced endothelium-dependent vasodilatation in thoracic aorta of early stage streptozotocin-induced diabetic mice. Acta Pharmcol Sin 2003;24:422–428.

Chiou WF, Huang YL, Chen CF, Chen CC. Vasorelaxing effect of coumarins from Cnidium monnieri on rabbit corpus cavernosum. Planta Med 2001;67:282–284.

He JY, Zhang W, He LC, Cao YX. Imperatorin induces vasodilatation possibly via inhibiting voltage dependent calcium channel and receptor-mediated Ca2+ influx and release. Eur J Pharmacol 2007;573:170–175.

Plane F, Wiley KE, Jeremy JY, Cohen RA, Garland CJ, Garland CJ. Evidence that different mechanisms underlie smooth muscle relaxation to nitric oxide and nitric oxide donors in the rabbit isolated carotid artery. Br J Pharmacol 1998;123:1351–1358.

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Experiment and Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences

Department of Pharmacology, College of Medicine, Xi’an Jiaotong University

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Molecular Research in Traditional Chinese Medicine Group, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology & Immunology, Medical University of Vienna

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