Effects of Xinjining (心悸宁) extract on inward rectifier potassium current in ventricular myocytes of guinea pig

Ming-jun Zhu; Guo-juan Wang; Yong-xia Wang; Jie-lin Pu; Hong-jun Liu; Hai-bin Yu

doi:10.1007/s11655-010-0061-8

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Effects of Xinjining (心悸宁) extract on inward rectifier potassium current in ventricular myocytes of guinea pig

Updated：2021-08-27

- Effects of Xinjining (心悸宁) extract on inward rectifier potassium current in ventricular myocytes of guinea pig
- Chinese Journal of Integrative Medicine Vol. 16, Issue 1, Pages: 61-65(2010)
- Affiliations：
  
  1. First Affiliated Hospital of Henan College of Traditional Chinese Medcine,Zhengzhou,China
  2. Fuwai Hospital of Cardiovascular Disease, Chinese Academy of Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Fund for Innovation of Phenom of Henan Province (No. 0521002400)
- DOI：10.1007/s11655-010-0061-8
  CLC：
- Published：2010，
  
  Published Online：4 February 2010，
Scan for full text
Zhu, Mj., Wang, Gj., Wang, Yx. et al. Effects of Xinjining (心悸宁) extract on inward rectifier potassium current in ventricular myocytes of guinea pig., Chin. J. Integr. Med. 16, 61–65 (2010). https://doi.org/10.1007/s11655-010-0061-8

Ming-jun Zhu, Guo-juan Wang, Yong-xia Wang, et al. Effects of Xinjining (心悸宁) extract on inward rectifier potassium current in ventricular myocytes of guinea pig. [J]. Chinese Journal of Integrative Medicine 16(1):61-65(2010)
Zhu, Mj., Wang, Gj., Wang, Yx. et al. Effects of Xinjining (心悸宁) extract on inward rectifier potassium current in ventricular myocytes of guinea pig., Chin. J. Integr. Med. 16, 61–65 (2010). https://doi.org/10.1007/s11655-010-0061-8 DOI：

Ming-jun Zhu, Guo-juan Wang, Yong-xia Wang, et al. Effects of Xinjining (心悸宁) extract on inward rectifier potassium current in ventricular myocytes of guinea pig. [J]. Chinese Journal of Integrative Medicine 16(1):61-65(2010) DOI： 10.1007/s11655-010-0061-8.

摘要

To study the effect of Xinjining extract (心悸宁

XJN) on inward rectifier potassium current (IK1) in ventricular myocyte (VMC) of guinea pigs and its anti-arrhythmic mechanism on ion channel level. Single VMC was enzymatically isolated by zymolisis

and whole-cell patch clamp recording technique was used to record the Ik1 in VMC irrigated with XJN of different concentrations (1.25

2.50

5.00 g/L; six samples for each). The stable current and conductance of the inward component of IK1 as well as the outward component of peak IK1 and conductance of it accordingly was recorded when the test voltage was set on −110 mV. The suppressive rate of XJN on the inward component of IK1 was 9.54%±5.81%

34.82%±15.03%

and 59.52%± 25.58% with a concentration of 1.25

2.50

and 5.00 g/L

respectively

and that for the outward component of peak IK1 was 23.94%±7.45%

52.98%±19.62%

and 71.42%±23.01%

respectively (all P<0.05). Moreover

different concentrations of XJN also showed effects for reducing IK1 conductance. XJN has inhibitory effect on IK1 in guinea pig’s VMC

and that of the same concentration shows stronger inhibition on outward component than on inward component

which may be one of the mechanisms of its anti-arrhythmic effect.

Abstract

To study the effect of Xinjining extract (心悸宁

XJN) on inward rectifier potassium current (IK1) in ventricular myocyte (VMC) of guinea pigs and its anti-arrhythmic mechanism on ion channel level. Single VMC was enzymatically isolated by zymolisis

and whole-cell patch clamp recording technique was used to record the Ik1 in VMC irrigated with XJN of different concentrations (1.25

2.50

34.82%±15.03%

and 59.52%± 25.58% with a concentration of 1.25

2.50

and 5.00 g/L

respectively

and that for the outward component of peak IK1 was 23.94%±7.45%

52.98%±19.62%

and 71.42%±23.01%

respectively (all P<0.05). Moreover

different concentrations of XJN also showed effects for reducing IK1 conductance. XJN has inhibitory effect on IK1 in guinea pig’s VMC

and that of the same concentration shows stronger inhibition on outward component than on inward component

which may be one of the mechanisms of its anti-arrhythmic effect.

关键词

Xinjining extractcardiac arrhythmiainward rectifier potassium current

Keywords

Xinjining extractcardiac arrhythmiainward rectifier potassium current

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Beijing University of Chinese Medicine

Department of Cardiology, Dongfang Hospital, Beijing University of Chinese Medicine

Beijing Institute of Traditional Chinese Medicine, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University

Scientific Research Division, Dongfang Hospital, Beijing University of Chinese Medicine

Intensive Care Unit, Dongfang Hospital, Beijing University of Chinese Medicine

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