Effect and mechanism of panaxoside Rg1 on neovascularization in myocardial infarction rats

Xiao-dong Wang; Tian-xiang Gu; En-yi Shi; Chun-mao Lu; Chun Wang

doi:10.1007/s11655-010-0162-4

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Effect and mechanism of panaxoside Rg1 on neovascularization in myocardial infarction rats

Updated：2021-08-27

- Effect and mechanism of panaxoside Rg1 on neovascularization in myocardial infarction rats
- Chinese Journal of Integrative Medicine Vol. 16, Issue 2, Pages: 162-166(2010)
- Affiliations：
  
  Department of Cardiac Surgery, the First Affiliated Hospital, China Medical University,Shenyang,China
- Author bio：
- Funds：
  
  Supported by the Research Project of Education Department of Liaoning Province (No. 2004C050) in 2005 and Project of Technology Department of Liaoning Province (No. 2006401013-2) in 2006.
- DOI：10.1007/s11655-010-0162-4
  CLC：
- Published：2010，
  
  Published Online：16 May 2010，
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Wang, Xd., Gu, Tx., Shi, Ey. et al. Effect and mechanism of panaxoside Rg1 on neovascularization in myocardial infarction rats., Chin. J. Integr. Med. 16, 162–166 (2010). https://doi.org/10.1007/s11655-010-0162-4

Xiao-dong Wang, Tian-xiang Gu, En-yi Shi, et al. Effect and mechanism of panaxoside Rg1 on neovascularization in myocardial infarction rats. [J]. Chinese Journal of Integrative Medicine 16(2):162-166(2010)
Wang, Xd., Gu, Tx., Shi, Ey. et al. Effect and mechanism of panaxoside Rg1 on neovascularization in myocardial infarction rats., Chin. J. Integr. Med. 16, 162–166 (2010). https://doi.org/10.1007/s11655-010-0162-4 DOI：

Xiao-dong Wang, Tian-xiang Gu, En-yi Shi, et al. Effect and mechanism of panaxoside Rg1 on neovascularization in myocardial infarction rats. [J]. Chinese Journal of Integrative Medicine 16(2):162-166(2010) DOI： 10.1007/s11655-010-0162-4.

摘要

To investigate the effects and mechanisms of panaxoside Rg1 on the new vessel formation in acute myocardial infarction (AMI) rats. The AMI model of male Sprague-Dawley (SD) rats was established

and rats were randomly divided into the AMI model group

the treatment group of panaxoside Rg1

the placebo group and the treatment group of panaxoside Rg1 plus rapamycin. Cardiac creatases were determined with 1 mL blood drawn from vena caudalis of the rats 48 h after the model was successfully made. After 4 weeks

Evans blue was injected into the aorta roots of the rats

and then

red tetrazoline was dyed again and the myocardial infarction area was evaluated. The microvessel density (MVD) of infarction area was determined by the immunohistochemistry of CD31; enzyme-linked immunosorbent assay (ELISA) was used to detect the protein content of CD31 and hypoxia inducible factor-1α (HIF-1α) of the infarction area. The MVD in the infarction area and the contents of CD31 and HIF-1α in the Rg1 treatment group were higher than those in the AMI model group significantly (P<0.05). The cardiac creatase and infarction area were lower in the Rg1 treatment group than those in the AMI model group significantly (P<0.05). The above effects

however

disappeared when rapamycin

the antagonist of mammalian target of rapamycin (mTOR)

was administered simultaneously. Panaxoside Rg1 could increase the expression of HIF-1α and CD31 of myocardium and stimulate the angiogenesis. The above mentioned role of panaxoside Rg1 might be related to the excitation of mTOR receptor.

Abstract

To investigate the effects and mechanisms of panaxoside Rg1 on the new vessel formation in acute myocardial infarction (AMI) rats. The AMI model of male Sprague-Dawley (SD) rats was established

and rats were randomly divided into the AMI model group

the treatment group of panaxoside Rg1

Evans blue was injected into the aorta roots of the rats

and then

however

disappeared when rapamycin

the antagonist of mammalian target of rapamycin (mTOR)

关键词

ginsenoside Rg1mTOR proteincollateral circulationsignal transductionmyocardial infarction

Keywords

ginsenoside Rg1mTOR proteincollateral circulationsignal transductionmyocardial infarction

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Department of Integrative Cardiology, China-Japan Friendship Hospital

Department of Cardiology, China-Japan Friendship Hospital

Department of Biochemistry, Institute of Basic Medicine, Shanghai University of Traditional Chinese Medicine

Department of Integrated Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Tecnology

Department of Laboratory, Ruijin Hospital Affiliated to the Medical College of Shanghai Jiao Tong University

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