Effects of Huanshuai Recipe Oral Liquid (缓衰口服液) on restructuring glomerular microvasculature and expression of vascular endothelial growth factor in subtotal nephrectomized rats

Shen Li; Xiang-rong Rao; Jin-ye Song; Li-qiang Meng; Lei Qu; Xiao-mei Li

doi:10.1007/s11655-010-0239-4

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Effects of Huanshuai Recipe Oral Liquid (缓衰口服液) on restructuring glomerular microvasculature and expression of vascular endothelial growth factor in subtotal nephrectomized rats

Updated：2021-08-27

- Effects of Huanshuai Recipe Oral Liquid (缓衰口服液) on restructuring glomerular microvasculature and expression of vascular endothelial growth factor in subtotal nephrectomized rats
- Chinese Journal of Integrative Medicine Vol. 16, Issue 3, Pages: 239-246(2010)
- Affiliations：
  
  1. Guang’ anmen Hospital, China Academy of Chinese Medical Sciences,Beijing,China
  2. Department of Renal Nephrology, Peking University First Hospital, Institute of Nephrology, Peking University and Key Lab of Public Health Ministry of China,Beijing,China
- Author bio：
- Funds：
  
  Supported by Special Projects of Chinese Medicine Science and Technology Research, State Administration of Traditional Chinese Medicine (No. 04-05JQ07)
- DOI：10.1007/s11655-010-0239-4
  CLC：
- Published：2010，
  
  Published Online：8 August 2010，
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Li, S., Rao, Xr., Song, Jy. et al. Effects of Huanshuai Recipe Oral Liquid (缓衰口服液) on restructuring glomerular microvasculature and expression of vascular endothelial growth factor in subtotal nephrectomized rats., Chin. J. Integr. Med. 16, 239–246 (2010). https://doi.org/10.1007/s11655-010-0239-4

Shen Li, Xiang-rong Rao, Jin-ye Song, et al. Effects of Huanshuai Recipe Oral Liquid (缓衰口服液) on restructuring glomerular microvasculature and expression of vascular endothelial growth factor in subtotal nephrectomized rats. [J]. Chinese Journal of Integrative Medicine 16(3):239-246(2010)
Li, S., Rao, Xr., Song, Jy. et al. Effects of Huanshuai Recipe Oral Liquid (缓衰口服液) on restructuring glomerular microvasculature and expression of vascular endothelial growth factor in subtotal nephrectomized rats., Chin. J. Integr. Med. 16, 239–246 (2010). https://doi.org/10.1007/s11655-010-0239-4 DOI：

Shen Li, Xiang-rong Rao, Jin-ye Song, et al. Effects of Huanshuai Recipe Oral Liquid (缓衰口服液) on restructuring glomerular microvasculature and expression of vascular endothelial growth factor in subtotal nephrectomized rats. [J]. Chinese Journal of Integrative Medicine 16(3):239-246(2010) DOI： 10.1007/s11655-010-0239-4.

摘要

To explore the effects and significance of Huanshuai Recipe Oral Liquid (缓衰口服液

HSR)

a formula with supplementing qi

nourishing blood and activating blood on restructuring glomerular microvasculature and expression of vascular endothelial growth factor (VEGF) in subtotal nephrectomized (SNX) rats. A total of 76 male Wistar rats were randomly divided into four groups: 16 in the sham-operated group and fed with tap water 10 mL/kg per day; 20 in the model group were operated with 5/6 SNX and fed with tap water 10 mL/ kg per day; 20 SNX rats in the HSR group were treated with HSR 10 mL/kg per day; 20 SNX rats in the losartan group were treated with losartan 40 mg/kg per day. Serum creatinine (SCr) and urinary protein excretion (Upro) were examined at the 2nd

4th

8th

and 12th weeks of the treatment

and the remnant kidneys were harvested. Changes in histological microstructure were evaluated using light microscopy

and the expression of VEGF was detected by using ELISA. Upro

microvasculature injury and glomerulosclerosis were found to be alleviated in HSR and Losartan groups

respectively. The change of VEGF expression showed positive correlation with glomerular capillary area and peritubular capillary number (r=0.448

r=0.422

P<0.01)

but negative correlation with that of SCr and Upro (r=−0.592

r=−0.481

P<0.01). HSR could regulate the VEGF expression

reduce the loss of microvasculature

which demonstrated similar renal protective effects to losartan in SNX rats. Examination of Chinese herbal medicine influence on VEGF signaling and restructuring renal microvasculature may elucidate the molecular mechanism of renal protection to a certain degree.

Abstract

To explore the effects and significance of Huanshuai Recipe Oral Liquid (缓衰口服液

HSR)

a formula with supplementing qi

4th

8th

and 12th weeks of the treatment

and the remnant kidneys were harvested. Changes in histological microstructure were evaluated using light microscopy

and the expression of VEGF was detected by using ELISA. Upro

microvasculature injury and glomerulosclerosis were found to be alleviated in HSR and Losartan groups

respectively. The change of VEGF expression showed positive correlation with glomerular capillary area and peritubular capillary number (r=0.448

r=0.422

P<0.01)

but negative correlation with that of SCr and Upro (r=−0.592

r=−0.481

P<0.01). HSR could regulate the VEGF expression

reduce the loss of microvasculature

关键词

Huanshuai Recipe Oral Liquidrestructuring glomerular microvasculaturevascular endothelial growth factor

Keywords

Huanshuai Recipe Oral Liquidrestructuring glomerular microvasculaturevascular endothelial growth factor

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