Shenmai Injection (参麦注射液) inhibiting the extracellular signal regulated kinase-induced human airway smooth muscle proliferation in asthma

Li-min Zhao; Li-jun Ma; Luo-xian Zhang; Ji-zhen Wu

doi:10.1007/s11655-010-0505-1

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Shenmai Injection (参麦注射液) inhibiting the extracellular signal regulated kinase-induced human airway smooth muscle proliferation in asthma

Updated：2021-08-27

- Shenmai Injection (参麦注射液) inhibiting the extracellular signal regulated kinase-induced human airway smooth muscle proliferation in asthma
- Chinese Journal of Integrative Medicine Vol. 16, Issue 4, Pages: 331-336(2010)
- Affiliations：
  
  Department of Respiratory Diseases, Henan Province People’s Hospital,Zhengzhou,China
- Author bio：
- Funds：
  
  Supported by the Key Project in Science and Technology of Henan Province (No. 072300450100) and Project of High and New Technology Development of Health Department in Henan Province (No. 20060140)
- DOI：10.1007/s11655-010-0505-1
  CLC：
- Published：2010，
  
  Published Online：10 August 2010，
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Zhao, Lm., Ma, Lj., Zhang, Lx. et al. Shenmai Injection (参麦注射液) inhibiting the extracellular signal regulated kinase-induced human airway smooth muscle proliferation in asthma., Chin. J. Integr. Med. 16, 331–336 (2010). https://doi.org/10.1007/s11655-010-0505-1

Li-min Zhao, Li-jun Ma, Luo-xian Zhang, et al. Shenmai Injection (参麦注射液) inhibiting the extracellular signal regulated kinase-induced human airway smooth muscle proliferation in asthma. [J]. Chinese Journal of Integrative Medicine 16(4):331-336(2010)
Zhao, Lm., Ma, Lj., Zhang, Lx. et al. Shenmai Injection (参麦注射液) inhibiting the extracellular signal regulated kinase-induced human airway smooth muscle proliferation in asthma., Chin. J. Integr. Med. 16, 331–336 (2010). https://doi.org/10.1007/s11655-010-0505-1 DOI：

Li-min Zhao, Li-jun Ma, Luo-xian Zhang, et al. Shenmai Injection (参麦注射液) inhibiting the extracellular signal regulated kinase-induced human airway smooth muscle proliferation in asthma. [J]. Chinese Journal of Integrative Medicine 16(4):331-336(2010) DOI： 10.1007/s11655-010-0505-1.

摘要

To investigate the relationship between the proliferation of sensitized human airway smooth muscle cells (HASMCs) and the expression of extracellular signal regulated kinase (ERK) and the effect of Shenmai Injection (参麦注射液

SMI) on HASMCs. The HASMCs cultured in vitro were divided into three groups: (1) control group; (2) sensitized group: containing 10% asthmatic serum; (3) SMI group: further divided into three different concentration subgroups interferred with 10 μL/mL

50 μL/mL

and 100 μL/mL SMI

respectively. The proliferation of HASMCs was detected using MTT method

the expression of proliferating cell nucleus antigen (PCNA) in HASMCs was detected using immunocytochemical staining

and the expression of phosphoration-ERK1/2 (p-ERK1/2) protein was detected using Western-blot. After passive sensitization

: the optical density value (A A490 value) of HASMCs was significantly increased from 0.366±0.086 to 0.839± 0.168 (P<0.05). In addition

the expression of PCNA was significantly increased from 28.7%±5.9% in the control group to 69.8%±7.5% in the sensitized group (P<0.05). At the same time

the expression of p-ERK1/2 in passively sensitized HASMCs was significantly increased compared with the control group (all P<0.05). After application of 10 μL/mL

50 μL/mL

and 100 μL/mL SMI to the cultured media of passively sensitized group

the A570 value was significantly decreased from 0.839±0.168 to 0.612±0.100

0.412±0.092

and 0.339±0.077

respectively (P<0.05). Moreover

the expression of PCNA was significantly decreased from 69.8%±7.5% to 57.8%±6.2%

40.7%±5.4%

and 26.1%±5.2%

respectively. At the same time

the expression of p-ERK1/2 in each SMI group was significantly decreased compared with the sensitized group (all P<0.05). ERK signal transduction pathway may be involved in the airway remodeling in asthma. The expression of ERK can be inhibited by SMI in a dose-dependent manner

thus preventing the proliferation of HASMCs.

Abstract

50 μL/mL

and 100 μL/mL SMI

respectively. The proliferation of HASMCs was detected using MTT method

the expression of proliferating cell nucleus antigen (PCNA) in HASMCs was detected using immunocytochemical staining

and the expression of phosphoration-ERK1/2 (p-ERK1/2) protein was detected using Western-blot. After passive sensitization

: the optical density value (A A490 value) of HASMCs was significantly increased from 0.366±0.086 to 0.839± 0.168 (P<0.05). In addition

the expression of PCNA was significantly increased from 28.7%±5.9% in the control group to 69.8%±7.5% in the sensitized group (P<0.05). At the same time

the expression of p-ERK1/2 in passively sensitized HASMCs was significantly increased compared with the control group (all P<0.05). After application of 10 μL/mL

50 μL/mL

and 100 μL/mL SMI to the cultured media of passively sensitized group

the A570 value was significantly decreased from 0.839±0.168 to 0.612±0.100

0.412±0.092

and 0.339±0.077

respectively (P<0.05). Moreover

the expression of PCNA was significantly decreased from 69.8%±7.5% to 57.8%±6.2%

40.7%±5.4%

and 26.1%±5.2%

respectively. At the same time

thus preventing the proliferation of HASMCs.

关键词

Shenmai injectionairway smooth muscle cellsextracellular signal regulated kinaseairway remodeling

Keywords

Shenmai injectionairway smooth muscle cellsextracellular signal regulated kinaseairway remodeling

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