Effects of Bushen Tongmai Recipe (补肾通脉方) on protein kinase Bα expression in polycystic ovary rats with insulin resistance

Qiong Li; Dong-mei Huang; Fu-er Lu; Yang Xie; Li-jun Xu; Xin Zou; Di Gong; Zeng-si Wang

doi:10.1007/s11655-010-0515-z

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Effects of Bushen Tongmai Recipe (补肾通脉方) on protein kinase Bα expression in polycystic ovary rats with insulin resistance

Updated：2021-08-27

- Effects of Bushen Tongmai Recipe (补肾通脉方) on protein kinase Bα expression in polycystic ovary rats with insulin resistance
- Chinese Journal of Integrative Medicine Vol. 16, Issue 4, Pages: 324-330(2010)
- Affiliations：
  
  Institute of Integrated Traditional and Western Medicine, Affiliated to Tongji Hospital, Tongji Medical College, Huazhong University of Sciences and Technology,Wuhan,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30600838)
- DOI：10.1007/s11655-010-0515-z
  CLC：
- Published：2010，
  
  Published Online：10 August 2010，
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Li, Q., Huang, Dm., Lu, Fe. et al. Effects of Bushen Tongmai Recipe (补肾通脉方) on protein kinase Bα expression in polycystic ovary rats with insulin resistance., Chin. J. Integr. Med. 16, 324–330 (2010). https://doi.org/10.1007/s11655-010-0515-z

Qiong Li, Dong-mei Huang, Fu-er Lu, et al. Effects of Bushen Tongmai Recipe (补肾通脉方) on protein kinase Bα expression in polycystic ovary rats with insulin resistance. [J]. Chinese Journal of Integrative Medicine 16(4):324-330(2010)
Li, Q., Huang, Dm., Lu, Fe. et al. Effects of Bushen Tongmai Recipe (补肾通脉方) on protein kinase Bα expression in polycystic ovary rats with insulin resistance., Chin. J. Integr. Med. 16, 324–330 (2010). https://doi.org/10.1007/s11655-010-0515-z DOI：

Qiong Li, Dong-mei Huang, Fu-er Lu, et al. Effects of Bushen Tongmai Recipe (补肾通脉方) on protein kinase Bα expression in polycystic ovary rats with insulin resistance. [J]. Chinese Journal of Integrative Medicine 16(4):324-330(2010) DOI： 10.1007/s11655-010-0515-z.

摘要

To observe the effects of Bushen Tongmai Recipe (补肾通脉方

BSTMR) on mRNA and protein expressions of protein kinase B alpha (PKB α) in hepatic

adipose

muscular

and ovarian tissues of polycystic ovary (PCO) rats with insulin resistance (IR) and to explore the possible molecular mechanism of BSTMR in treating IR and ovulation dysfunction. Female 22-day-old SD rats were injected subcutaneously with sodium prasterone sulfate (9 mg·100g−1·d−1) for 20 days and fed with high-fat diet for 80 days to induce PCO rats with IR. Then

the PCO rats were randomly divided into the model group (n=23) and the treated group (n=21). The treated group was administered with BSTMR for 2 weeks. Meanwhile

a group with 15 rats of the same age was used as the control group. The histological changes in the ovaries were examined. Fasting blood glucose (FBG) was determined by the glucose oxidase method. Serum fasting insulin (Fins) was determined by radioimmunoassay (RIA). The mRNA level of PKBα was measured by reverse transcription polymerase chain reaction (RT-PCR). Immunohistochemistry staining and Western blot analysis were employed to detect the protein expression in target tissues. Compared with the control group

the ovaries in the model group showed multiple follicular cysts

levels of FBG and Fins in the model group increased markedly (P<0.05 or P<0.01

respectively)

and the insulin sensitive index (ISI) decreased obviously (P<0.01). The mRNA and protein expressions of PKBα in target tissues in the model group were dramatically lower than those in the control group (P<0.05 or P<0.01). Compared with the model group

the stratum granulosum of the ovarian follicle in the treated group increased markedly

the level of Fins in the treated group decreased obviously (P<0.01)

ISI in the treated group improved markedly (P<0.01)

and the mRNA and protein expressions of PKBα in target tissues of the treated rats were elevated significantly (P<0.05 or P<0.01). BSTMR could improve IR and ovulation dysfunction in PCO rats with IR

and its molecular mechanisms might be closely related with the elevation of mRNA and protein expressions of PKBα in target tissues of PCO rats with IR.

Abstract

To observe the effects of Bushen Tongmai Recipe (补肾通脉方

BSTMR) on mRNA and protein expressions of protein kinase B alpha (PKB α) in hepatic

adipose

muscular

the PCO rats were randomly divided into the model group (n=23) and the treated group (n=21). The treated group was administered with BSTMR for 2 weeks. Meanwhile

the ovaries in the model group showed multiple follicular cysts

levels of FBG and Fins in the model group increased markedly (P<0.05 or P<0.01

respectively)

the stratum granulosum of the ovarian follicle in the treated group increased markedly

the level of Fins in the treated group decreased obviously (P<0.01)

ISI in the treated group improved markedly (P<0.01)

and its molecular mechanisms might be closely related with the elevation of mRNA and protein expressions of PKBα in target tissues of PCO rats with IR.

关键词

polycystic ovaryinsulin resistanceBushen Tongmai Recipeprotein kinase Bα

Keywords

polycystic ovaryinsulin resistanceBushen Tongmai Recipeprotein kinase Bα

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Xiamen Diabetes Institute

Department of Internal Medicine, the First Affiliated Hospital of Xiamen University

Post-Graduate School, Fujian University of Traditional Chinese Medicine

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