Tumor interstitial fluid and postoperative recurrence of tumors: An experimental study for verifying hypothesis of “tumor-phlegm microenvironment”

Da-zhi Sun; Da-wei Ju; Jin He; Ye Lu; Feng Wu; Chang Li; Pin-kang Wei

doi:10.1007/s11655-010-0537-6

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Tumor interstitial fluid and postoperative recurrence of tumors: An experimental study for verifying hypothesis of “tumor-phlegm microenvironment”

Updated：2021-08-27

- Tumor interstitial fluid and postoperative recurrence of tumors: An experimental study for verifying hypothesis of “tumor-phlegm microenvironment”
- Chinese Journal of Integrative Medicine Vol. 16, Issue 5, Pages: 435-441(2010)
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, Shanghai Changzheng Hospital, the Second Military Medical University,Shanghai,China
  2. Department of Laboratory Digniostics, Shanghai Changzheng Hospital, the Second Military Medical University,Shanghai,China
  3. Department of Pathology, Shanghai Changzheng Hospital, the Second Military Medical University,Shanghai,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation (Major Research Project, No. 90709044) and Shanghai Changzheng Hospital “Three Major, Three Merit” Project Fund
- DOI：10.1007/s11655-010-0537-6
  CLC：
- Published：2010，
  
  Published Online：25 September 2010，
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Sun, Dz., Ju, Dw., He, J. et al. Tumor interstitial fluid and postoperative recurrence of tumors: An experimental study for verifying hypothesis of “tumor-phlegm microenvironment”., Chin. J. Integr. Med. 16, 435–441 (2010). https://doi.org/10.1007/s11655-010-0537-6

Da-zhi Sun, Da-wei Ju, Jin He, et al. Tumor interstitial fluid and postoperative recurrence of tumors: An experimental study for verifying hypothesis of “tumor-phlegm microenvironment”. [J]. Chinese Journal of Integrative Medicine 16(5):435-441(2010)
Sun, Dz., Ju, Dw., He, J. et al. Tumor interstitial fluid and postoperative recurrence of tumors: An experimental study for verifying hypothesis of “tumor-phlegm microenvironment”., Chin. J. Integr. Med. 16, 435–441 (2010). https://doi.org/10.1007/s11655-010-0537-6 DOI：

Da-zhi Sun, Da-wei Ju, Jin He, et al. Tumor interstitial fluid and postoperative recurrence of tumors: An experimental study for verifying hypothesis of “tumor-phlegm microenvironment”. [J]. Chinese Journal of Integrative Medicine 16(5):435-441(2010) DOI： 10.1007/s11655-010-0537-6.

摘要

To explore a method of extracting tumor interstitial fluid (TIF) which is similar to muddy phlegm in Chinese medicine (CM)

interleukin-8 (IL-8) in concentration was taken as the representative of the content of TIF

analyzed in the extracted TIF and the original tumor tissue

and examined to see whether TIF has an interfering effect on tumor recurrence. Tumor tissue was ground

centrifuged

and filtered for intercellular substances. Tumor-bearing Kunming S180 mice were raised for 21 days and then the tumors were removed to observe the influence of intervention with TIF

normal saline (NS) and a blank control on tumor recurrence. The content of IL-8 in the filtered and unfiltered tumor tissue was not significantly different (P>0.05). Postoperative tumor recurrence in TIF intervention group was significantly higher than that in the NS intervention and control groups (60%

12/20 vs. 20%

4/20. vs. 15%

3/20

χ2 =11.058

P<0.01). Tumor cells grew vigorously and infiltrated to muscular tissue in TIF intervention group. Large numbers of tumor cells were seen necrotic in the NS intervention group

and small numbers of tumor cells were seen necrotic in the blank control group. TIF can be effectively extracted by the means described. It does not contain tumor cells

but its contents such as IL-8 may stimulate tumor cell growth and promote postoperative tumor recurrence

which provided preliminary experimental basis for hypothesis of “tumor-phlegm microenvironment”.

Abstract

To explore a method of extracting tumor interstitial fluid (TIF) which is similar to muddy phlegm in Chinese medicine (CM)

interleukin-8 (IL-8) in concentration was taken as the representative of the content of TIF

analyzed in the extracted TIF and the original tumor tissue

and examined to see whether TIF has an interfering effect on tumor recurrence. Tumor tissue was ground

centrifuged

and filtered for intercellular substances. Tumor-bearing Kunming S180 mice were raised for 21 days and then the tumors were removed to observe the influence of intervention with TIF

12/20 vs. 20%

4/20. vs. 15%

3/20

χ2 =11.058

P<0.01). Tumor cells grew vigorously and infiltrated to muscular tissue in TIF intervention group. Large numbers of tumor cells were seen necrotic in the NS intervention group

and small numbers of tumor cells were seen necrotic in the blank control group. TIF can be effectively extracted by the means described. It does not contain tumor cells

but its contents such as IL-8 may stimulate tumor cell growth and promote postoperative tumor recurrence

which provided preliminary experimental basis for hypothesis of “tumor-phlegm microenvironment”.

关键词

tumor interstitial fluidmuddy phlegminterleukin-8tumor recurrence

Keywords

tumor interstitial fluidmuddy phlegminterleukin-8tumor recurrence

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Department of Pathology, Changzheng Hospital, Second Military Medical University

Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University

Institute of Traditional Chinese Medicine Research, Chinese People’s Liberation Army General Hospital

Department of Clinical Laboratory Medicine, Affiliate Hospital of Logistical College of Chinese People’s Armed Police Forces

Department of Clinical Laboratory Medicine, Chinese People’s Liberation Army General Hospital and Postgraduate Medical School

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