Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways

Xian-qian Li; Shi-he Shao; Gui-lian Fu; Xiao-hong Han; Hong Gao

doi:10.1007/s11655-010-0538-5

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Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways

Updated：2021-08-27

- Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways
- Chinese Journal of Integrative Medicine Vol. 16, Issue 5, Pages: 448-452(2010)
- Affiliations：
  
  1. Pathogenic Microorganism Department, School of Medical Science and Laboratory Medicine, Jiangsu University,Jiangsu Province,Zhenjiang,China
  2. Laboratory Center, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,China
  3. Department of Biochemistry and Molecular Biology, Department of Hematology and Oncology, University of Miami Miller School of Medicine,Miami,USA
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 2007Z002A), Grants of Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine and Grants from the Department of Biochemistry and Molecular Biology, Department of Hematology and Oncology, University of Miami Miller School of Medicine
- DOI：10.1007/s11655-010-0538-5
  CLC：
- Published：2010，
  
  Published Online：25 September 2010，
Scan for full text
Li, Xq., Shao, Sh., Fu, Gl. et al. Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways., Chin. J. Integr. Med. 16, 448–452 (2010). https://doi.org/10.1007/s11655-010-0538-5

Xian-qian Li, Shi-he Shao, Gui-lian Fu, et al. Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways. [J]. Chinese Journal of Integrative Medicine 16(5):448-452(2010)
Li, Xq., Shao, Sh., Fu, Gl. et al. Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways., Chin. J. Integr. Med. 16, 448–452 (2010). https://doi.org/10.1007/s11655-010-0538-5 DOI：

Xian-qian Li, Shi-he Shao, Gui-lian Fu, et al. Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways. [J]. Chinese Journal of Integrative Medicine 16(5):448-452(2010) DOI： 10.1007/s11655-010-0538-5.

摘要

To investigate the mechanism of norcantharidin (NCTD)-induced SMMC-7721 hepatoma cell apoptosis. SMMC-7721 cell growth inhibition was measured by the MTT method. Apoptosis was detected by Annexin V/propidium iodide staining. The mitochondrial membrane potential was measured by flow cytometry. Western blot analysis was used to evaluate the level of cytochrome c

caspase-3

AIF

Bcl-2 and Bax expression. NCTD inhibited SMMC-7721 cell growth in a time- and dose-dependent manner. The cells treated with NCTD showed the loss of mitochondrial membrane potential. The activities of caspase-3

cytochrome c

AIF

and Bax were up-regulated after NCTD treatment at different doses. The expression of Bcl-2 was decreased after treatment with NCTD. NCTD could induce SMMC-7721 cell apoptosis. The activation of the mitochondrial pathway was involved in the process of NCTD-induced SMMC-7721 cell apoptosis.

Abstract

caspase-3

AIF

cytochrome c

AIF

关键词

norcantharidinLiver Cancerapoptosismitochondrial pathway

Keywords

norcantharidinLiver Cancerapoptosismitochondrial pathway

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