Effect of Qingre Quyu Granule (清热祛瘀颗粒) on stabilizing plaques in the brachiocephalic artery of apolipoprotein E deficient mice

Yong Wang; Wen-li Cheng; Yuan-nan Ke; Zhe Cai; Li Chen; Yuan Xi; Pu Wang; Jian Guo; Hong Li; Cong-xin Huang

doi:10.1007/s11655-010-0545-6

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Effect of Qingre Quyu Granule (清热祛瘀颗粒) on stabilizing plaques in the brachiocephalic artery of apolipoprotein E deficient mice

Updated：2021-08-27

- Effect of Qingre Quyu Granule (清热祛瘀颗粒) on stabilizing plaques in the brachiocephalic artery of apolipoprotein E deficient mice
- Chinese Journal of Integrative Medicine Vol. 16, Issue 5, Pages: 442-447(2010)
- Affiliations：
  
  1. Department of Cardiology, People’s Hospital, Wuhan University,Wuhan,China
  2. National Integrative Medicine Center for Cardiovascular Diseases, China-Japan Friendship Hospital,Beijing,China
  3. Beijing University of Chinese Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Capital Medical Development Scientific Research Foundation (No. SF-2007-III-41)
- DOI：10.1007/s11655-010-0545-6
  CLC：
- Published：2010，
  
  Published Online：25 September 2010，
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Wang, Y., Cheng, Wl., Ke, Yn. et al. Effect of Qingre Quyu Granule (清热祛瘀颗粒) on stabilizing plaques in the brachiocephalic artery of apolipoprotein E deficient mice., Chin. J. Integr. Med. 16, 442–447 (2010). https://doi.org/10.1007/s11655-010-0545-6

Yong Wang, Wen-li Cheng, Yuan-nan Ke, et al. Effect of Qingre Quyu Granule (清热祛瘀颗粒) on stabilizing plaques in the brachiocephalic artery of apolipoprotein E deficient mice. [J]. Chinese Journal of Integrative Medicine 16(5):442-447(2010)
Wang, Y., Cheng, Wl., Ke, Yn. et al. Effect of Qingre Quyu Granule (清热祛瘀颗粒) on stabilizing plaques in the brachiocephalic artery of apolipoprotein E deficient mice., Chin. J. Integr. Med. 16, 442–447 (2010). https://doi.org/10.1007/s11655-010-0545-6 DOI：

Yong Wang, Wen-li Cheng, Yuan-nan Ke, et al. Effect of Qingre Quyu Granule (清热祛瘀颗粒) on stabilizing plaques in the brachiocephalic artery of apolipoprotein E deficient mice. [J]. Chinese Journal of Integrative Medicine 16(5):442-447(2010) DOI： 10.1007/s11655-010-0545-6.

摘要

To investigate the effect of Qingre Quyu Granule (清热祛瘀颗粒

QRQYG) on stabilizing vulnerable plaques in apolipoprotein E (ApoE) deficient mice. Seventy-two male ApoE deficient mice were given a high-fat diet from 6 weeks of age. At the 16th week

all the mice were randomized into 3 groups: the QRQYG group

the simvastatin group

and the control group. Sixteen weeks after administration of 0.9 g/kg QRQYG

3 mg/kg simvastatin or 10 mg/kg sodium chloride per day to the respective groups

the animals were euthanized. The pathological morphologic changes in the vulnerable plaques were evaluated

the matrix metalloprotease-9 (MMP-9) expression was measured by immunohistofluorescence

the soluble intercellular adhesion molecule 1 (ICAM-1) was determined by ELISA

the nuclear factor kappaB (NF-κB) subunit p65 was measured by quantitative RT-PCR

and

finally

thrombospondin-1 (TSP-1) was determined by the immunohistochemical method. The plaque cross-sectional area in the brachiocephalic artery (23.7%

P<0.01)

the lipid core of the plaque (43.1%±3.1%)

and the number of buried fibrotic caps of the plaque were significantly decreased in the QRQYG group compared to the control group (both P<0.01); furthermore

the thickness of the fibrotic cap of the plaque increased and the intra-plaque hemorrhage of the plaque decreased. The serum soluble ICAM-1 (27.1±5.1 μg/mL)

the protein expression of MMP-9 and TSP-1 and the p65 mRNA expression increased in the QRQYG group in comparison with the control group (P<0.05 or P<0.01). QRQYG could stabilize the vulnerable plaque through inhibition of the inflammatory response.

Abstract

To investigate the effect of Qingre Quyu Granule (清热祛瘀颗粒

QRQYG) on stabilizing vulnerable plaques in apolipoprotein E (ApoE) deficient mice. Seventy-two male ApoE deficient mice were given a high-fat diet from 6 weeks of age. At the 16th week

all the mice were randomized into 3 groups: the QRQYG group

the simvastatin group

and the control group. Sixteen weeks after administration of 0.9 g/kg QRQYG

3 mg/kg simvastatin or 10 mg/kg sodium chloride per day to the respective groups

the animals were euthanized. The pathological morphologic changes in the vulnerable plaques were evaluated

the matrix metalloprotease-9 (MMP-9) expression was measured by immunohistofluorescence

the soluble intercellular adhesion molecule 1 (ICAM-1) was determined by ELISA

the nuclear factor kappaB (NF-κB) subunit p65 was measured by quantitative RT-PCR

and

finally

thrombospondin-1 (TSP-1) was determined by the immunohistochemical method. The plaque cross-sectional area in the brachiocephalic artery (23.7%

P<0.01)

the lipid core of the plaque (43.1%±3.1%)

and the number of buried fibrotic caps of the plaque were significantly decreased in the QRQYG group compared to the control group (both P<0.01); furthermore

the thickness of the fibrotic cap of the plaque increased and the intra-plaque hemorrhage of the plaque decreased. The serum soluble ICAM-1 (27.1±5.1 μg/mL)

关键词

Vulnerable Plaquematrix metalloprotease-9intercellular adhesion molecule-1nuclear factor kappaBthrombospondin-1atherosclerosis

Keywords

Vulnerable Plaquematrix metalloprotease-9intercellular adhesion molecule-1nuclear factor kappaBthrombospondin-1atherosclerosis

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Department of Traditional Chinese Medicine, Shanghai No. 6 People’s Hospital, Affiliated to Shanghai Jiaotong University

National Integrative Center for Cardiovascular Diseases, China-Japan Friendship Hospital

Shandong University of Chinese Medicine

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Lab, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou

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