Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome

Mei Xue; Hui-jun Yin; Cai-feng Wu; Xiao-juan Ma; Chun-yu Guo; Ye Huang; Da-zhuo Shi; Ke-ji Chen

doi:10.1007/s11655-011-0604-7

Your Location：

Home >

Browse articles >

Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome

Updated：2021-08-27

- Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome
- Chinese Journal of Integrative Medicine Vol. 19, Issue 1, Pages: 42-47(2013)
- Affiliations：
  
  Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by National Basic Research Program of China (No. 2006CB504803 and No. 81030063)
- DOI：10.1007/s11655-011-0604-7
  CLC：
- Published：2013，
  
  Published Online：8 December 2011，
Scan for full text
Xue, M., Yin, Hj., Wu, Cf. et al. Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome., Chin. J. Integr. Med. 19, 42–47 (2013). https://doi.org/10.1007/s11655-011-0604-7

Mei Xue, Hui-jun Yin, Cai-feng Wu, et al. Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome. [J]. Chinese Journal of Integrative Medicine 19(1):42-47(2013)
Xue, M., Yin, Hj., Wu, Cf. et al. Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome., Chin. J. Integr. Med. 19, 42–47 (2013). https://doi.org/10.1007/s11655-011-0604-7 DOI：

Mei Xue, Hui-jun Yin, Cai-feng Wu, et al. Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome. [J]. Chinese Journal of Integrative Medicine 19(1):42-47(2013) DOI： 10.1007/s11655-011-0604-7.

摘要

To observe the regulatory effect of Chinese drugs for activating blood circulation (ABC) and for activating blood circulation and detoxifying (ABCD) on indices of thrombosis

inflammatory reaction

and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome. Fifty-four rabbits were randomized into the normal control group

model group

simvastatin group (simvastatin

0.93 mg/kg per day)

ABC group [Xiongshao Capsule (芎芍胶囊)

0.07 g/kg per day]

and ABCD group [Xiongshao Capsule

0.07 g/kg per day

and Huanglian Capsule (黄连胶囊)

0.14 g/kg per day]. All except the normal control group received a single injection of bovine serum albumin and were fed with high-fat diets for 6 weeks. At the end of week 4 of giving high-fat diets

a dose of endoxitin was given by ear vein injection

and a randomized 2-week treatment was initiated. At the end of treatment

blood lipids

circulating endothelial cells

and the pathological changes of the aortic arch were assessed. The serum levels of matrix metalloproteinases (MMP-9)

tissue inhibitors to metalloproteinase (TIMP-1)

granule membrane protein-140 (GMP-140)

plasminogen activator inhibitor-1 (PAI-1)

high-sensitivity C-reactive protein (hs-CRP)

interleukin-6 (IL-6)

and tumor necrosis factor-α(TNF-α) were determined. Compared with the model group

ABCD group showed decreased serum triglyceride (TG) level

improvement in the pathological change in the aortic arch

and reduction in the number of circulating endothelial cells (4.00±1.41 per 0.9 μL for ABCD group vs 7.83±1.72 per 0.9 μL for the model group). In addition

the levels of serum GMP-140

PAI-1

and IL-6 in ABCD group were also significantly reduced [0.79±0.20 ng/mL

5.23±1.39 ng/mL

40.64±10.11 pg/mL for ABCD group vs 1.08±0.31 ng/mL

7.28±2.01 ng/mL

54.44±13.56 pg/mL for the model group

respectively

P<0.05]. A trend showing improvement in the indices of thrombosis

inflammatory reaction

and tissue damage was observed in the ABC group when compared to the model group

but the changes were not statistically significant (P>0.05). Chinese drugs for activating blood circulation and detoxifying have beneficial effects on regulating indices of thrombosis (GMP-140 and PAI-1) and inflammatory reaction (IL-6) in rabbit model with toxic-heat and blood stasis. The effect of the activating blood circulation and detoxifying drugs in regulating the levels of serum GMP-140

PAI-1

and IL-6 was superior to that of the activating blood circulation drugs.

Abstract

To observe the regulatory effect of Chinese drugs for activating blood circulation (ABC) and for activating blood circulation and detoxifying (ABCD) on indices of thrombosis

inflammatory reaction

and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome. Fifty-four rabbits were randomized into the normal control group

model group

simvastatin group (simvastatin

0.93 mg/kg per day)

ABC group [Xiongshao Capsule (芎芍胶囊)

0.07 g/kg per day]

and ABCD group [Xiongshao Capsule

0.07 g/kg per day

and Huanglian Capsule (黄连胶囊)

0.14 g/kg per day]. All except the normal control group received a single injection of bovine serum albumin and were fed with high-fat diets for 6 weeks. At the end of week 4 of giving high-fat diets

a dose of endoxitin was given by ear vein injection

and a randomized 2-week treatment was initiated. At the end of treatment

blood lipids

circulating endothelial cells

and the pathological changes of the aortic arch were assessed. The serum levels of matrix metalloproteinases (MMP-9)

tissue inhibitors to metalloproteinase (TIMP-1)

granule membrane protein-140 (GMP-140)

plasminogen activator inhibitor-1 (PAI-1)

high-sensitivity C-reactive protein (hs-CRP)

interleukin-6 (IL-6)

and tumor necrosis factor-α(TNF-α) were determined. Compared with the model group

ABCD group showed decreased serum triglyceride (TG) level

improvement in the pathological change in the aortic arch

and reduction in the number of circulating endothelial cells (4.00±1.41 per 0.9 μL for ABCD group vs 7.83±1.72 per 0.9 μL for the model group). In addition

the levels of serum GMP-140

PAI-1

and IL-6 in ABCD group were also significantly reduced [0.79±0.20 ng/mL

5.23±1.39 ng/mL

40.64±10.11 pg/mL for ABCD group vs 1.08±0.31 ng/mL

7.28±2.01 ng/mL

54.44±13.56 pg/mL for the model group

respectively

P<0.05]. A trend showing improvement in the indices of thrombosis

inflammatory reaction

and tissue damage was observed in the ABC group when compared to the model group

PAI-1

and IL-6 was superior to that of the activating blood circulation drugs.

关键词

activating blood circulationactivating blood circulation and detoxifyingthrombosistissue damageinflammatory reaction

Keywords

activating blood circulationactivating blood circulation and detoxifyingthrombosistissue damageinflammatory reaction

references

Zhang JC, Chen KJ, Zheng GJ, Zhang WG, Shi DZ, Yin HJ, et al. Regulatory effect of Chinese herbal compound for detoxifying and activating blood circulation on expression of NF-κB and MMP-9 in aorta of apolipoprotein E gene knocked-out mice. Chin J Integr Tradit West Med (Chin) 2007;27:40–44.

Zhang JC, Chen KJ, Liu JG, Zhang WG, Shi DZ, Liu LT, et al. Effect of assorted use of Chinese drugs for detoxifying and activating blood circulation on serum high sensitive C-reactive protein in apolipoprotein E gene knock-out mice. Chin J Integr Tradit West Med (Chin) 2008;28:330–333.

Zhou MX, Xu H, Chen KJ, Pan L, Wen C, Liu JG. Effects of some active ingredients of Chinese drugs for activating blood circulation and detoxicating on blood lipids and atherosclerotic plaque inflammatory reaction in ApoE-gene knock-out mice. Chin J Integr Tradit West Med (Chin) 2008;28:126–130.

Chen Q, ed. Methodological study of Chinese herbs pharmacology. Beijing: People’s Medical Publishing House;1996:8, 1103–1105.

Gan WJ, Liu JT, Lin R. The protective effect of quercetin on the vascular endothelial cells injured by homocysteine in rabbits. Chin Pharmacol Bull (Chin) 2004;20:647–651.

Sbarbati D, D Boer M, Marzilli M, Scarlattini M, Rossi G, JA van Mourik. Immunologic detection of endothelial cells in human whole blood. Blood 1991;77:764–769.

Ding J, Chen HY, Kong J, Lin GZ. Effect of Rosiglitazone on cardiovascular ultrastructure of atherosclerosis in rabbits. Chin J Gerontol (Chin) 2008;28(4):342–344.

Wang KF, Lu FE, Xu LJ, Zou X. Study on the dynamic changes of body temperature and plasma endotoxin levels in rabbits after infusion with endotoxin. Acta Univ Med Tongji (Chin) 2001;30:129–133.

Chen KJ, Xue M, Yin HJ. The relationship between platelet activation and coronary heart disease and blood-stasis syndrome. J Capitl Med Univ (Chin) 2008;29:266–269.

Chen LH, Lu GP, Wu CF, Gong LS. Effect of pravastatin on arterial gene expression of plasminogen activator inhibitor type 1 in atherosclerotic rabbits. Chin J Cardiol (Chin) 2001;29:115–117.

Ma XJ, Yin HJ, Chen KJ. Research progress of correlation between blood-stasis syndrome and inflammation. Chin J Integr Tradit West Med (Chin) 2007;27:669–672.

Bruno G, Todor R, Lewis I, Chyatte D. Vascular extracellular matrix remodeling in cerebral aneurysms. J Neurosurg 1998;89:431–440.

Ji Y, Zhang RY, Lu GP, Qi WH, Gao PJ, Zhu DL. Effect of simvastatin on the expressions of P-selectin and ICAM-1 in atherosclerotic iliac artery of rabbits. Chin J Geriatr (Chin) 2003;22:165–168.

Qin L, Zhu Y, Huang KX. Study on expression of plasminogen activator inhibitor type-1 and its correlation with cholesterolemia in the atherosclerotic rabbits. J Clin Cardiol (Chin) 2006;22:538–540.

Zhang L, Jiang YR, Xue M, Wu CF, Wang JS, Yin HJ. Effect of simvastatin on the atherosclerotic plaque stability and the angiogenesis in atherosclerotic plaque of rabbits. Chin Sci Bull (Chin) 2009;54:2228–2232.

Yin HK, Sun CK, Chang LT, Wu CJ, Chua S, Fu M. Strong suppression of high-sensitivity C-reactive protein level and its mediated pro-atherosclerotic effects with simvastatin: in vivo and in vitro studies. Int J Cardiol 2007;121:253–260.

FULL TEXT LINK

More>

Views

941

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Effect of Chinese herbal medicine for activating blood circulation and detoxifying on expression of inflammatory reaction and tissue damage related factors in experimental carotid artery thrombosis rats

Inhibitory effects of Qushuanling Capsule (祛栓灵胶囊) on thrombus formation and platelet aggregation in rats

Effect of Shengmai Injection (生脉注射液) on cardiac function and inflammatory reaction in patients with acute coronary syndrome

Effects of total flavone of Abelmoschl Manihot L. Medic on the function of platelets and its mechanism

Effect of Chinese herbs for activating blood circulation, removing stasis and supplementing Qi on the circulating endothelial cells in patients with unstable angina pectoris

Related Author

No data

Related Institution

Center of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences

Department of Pharmacology, College of Pharmaceutical Science, Soochow University

Experimental Center of Medical and Biological Teaching, Medical College of Soochow University

Institute of Pharmaceutical Research in Yangzhou University

Department of Internal Cardiology, Xinhua Hospital Affiliated to Shanghai Jiaotong Medical University School of Medicine

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰