Effects of Herba Epimedii and Fructus Ligustri lucidi on the transcription factors in hypothalamus of aged rats

Jing Cai; Teng Zheng; Lei Zhang; Yun Tian; Min-he Yang; Jian Du

doi:10.1007/s11655-011-0636-z

Your Location：

Home >

Browse articles >

Effects of Herba Epimedii and Fructus Ligustri lucidi on the transcription factors in hypothalamus of aged rats

Updated：2021-08-27

- Effects of Herba Epimedii and Fructus Ligustri lucidi on the transcription factors in hypothalamus of aged rats
- Chinese Journal of Integrative Medicine Vol. 17, Issue 10, Pages: 758-763(2011)
- Affiliations：
  
  1. Fujian Institute of Integrative Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. Fujian Entry-Exit Inspection and Quarantine Bureau of P. R. China,Fuzhou,China
  3. Fujian Normal University,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30672729), CHEN Ke-ji Development Fund of Integrative Medicine (No. CKJ2010025)
- DOI：10.1007/s11655-011-0636-z
  CLC：
- Published：2011，
  
  Published Online：26 March 2013，
Scan for full text
Cai, J., Zheng, T., Zhang, L. et al. Effects of Herba Epimedii and Fructus Ligustri lucidi on the transcription factors in hypothalamus of aged rats., Chin. J. Integr. Med. 17, 758–763 (2011). https://doi.org/10.1007/s11655-011-0636-z

Jing Cai, Teng Zheng, Lei Zhang, et al. Effects of Herba Epimedii and Fructus Ligustri lucidi on the transcription factors in hypothalamus of aged rats. [J]. Chinese Journal of Integrative Medicine 17(10):758-763(2011)
Cai, J., Zheng, T., Zhang, L. et al. Effects of Herba Epimedii and Fructus Ligustri lucidi on the transcription factors in hypothalamus of aged rats., Chin. J. Integr. Med. 17, 758–763 (2011). https://doi.org/10.1007/s11655-011-0636-z DOI：

Jing Cai, Teng Zheng, Lei Zhang, et al. Effects of Herba Epimedii and Fructus Ligustri lucidi on the transcription factors in hypothalamus of aged rats. [J]. Chinese Journal of Integrative Medicine 17(10):758-763(2011) DOI： 10.1007/s11655-011-0636-z.

摘要

To comparatively analyze the difference in the expression of 54 transcription factors in the hypothalamus using protein chips following the medication of Chinese drugs for Shen (肾)-tonification

Herba Epimedii

and Fructus Ligustri lucidi (FL) to aged rats. Wistar rats

aged 15 months of SPF grade

were randomized into three groups

three males and three females in each group. They were medicated with Herba Epimedii decoction (HED

0.14 g/kg)

Fructus Ligustri lucidi decoction (FLD

0.12 g/kg)

and distilled water

respectively

twice a day for 15 days. The rats were sacrificed at the morning of the 16th day 1 h after medication

and their hypothalamus was taken and made into homogenate under an ice-bath for detecting the expression of transcription factors with chip technique. The expressions of signal transduction and transcription activation factor-6 (Stat-6) and androgen receptor (AR) were up-regulated

and those of pre-B transcription factor1 (Pbx-1)

stat-1 and AP-2 were down-regulated in both HED and FLD treated groups

but these changes occurred mainly in female rats in the former while mainly in males in the latter. Chinese drugs for Shen-tonification could impact the expression of transcription factors in the hypothalamus of aged rats

dominantly on the neuro-endocrine factors responsible for the growth and development. The effects of drugs for tonifying Shen-yang and for tonifying Shen-yin are different

which is probably one of the pharmacological mechanisms of the Shen-tonifying drugs.

Abstract

To comparatively analyze the difference in the expression of 54 transcription factors in the hypothalamus using protein chips following the medication of Chinese drugs for Shen (肾)-tonification

Herba Epimedii

and Fructus Ligustri lucidi (FL) to aged rats. Wistar rats

aged 15 months of SPF grade

were randomized into three groups

three males and three females in each group. They were medicated with Herba Epimedii decoction (HED

0.14 g/kg)

Fructus Ligustri lucidi decoction (FLD

0.12 g/kg)

and distilled water

respectively

twice a day for 15 days. The rats were sacrificed at the morning of the 16th day 1 h after medication

and those of pre-B transcription factor1 (Pbx-1)

stat-1 and AP-2 were down-regulated in both HED and FLD treated groups

dominantly on the neuro-endocrine factors responsible for the growth and development. The effects of drugs for tonifying Shen-yang and for tonifying Shen-yin are different

which is probably one of the pharmacological mechanisms of the Shen-tonifying drugs.

关键词

Herba EpimediiFructus Ligustri luciditranscription factoraged rathypothalamus

Keywords

Herba EpimediiFructus Ligustri luciditranscription factoraged rathypothalamus

references

Zhang C, Wang JG, Yang T, Liu GS, Chen QX. Effect of Bushen Yinao Tablet on physiological functions and gene expression in SAMP8 mice. Chin J Integr Tradit West Med (Chin) 2006;26(Suppl):25–30.

David S. Latchman, ed. Eukaryotic transcription factors. 5th ed. Beijing: Science Press; 2008:12–15.

Liu XY, Xia SJ, Shen ZY. Changes in nuclear factors expression in aging. Chin J Geriatr (Chin) 2007;26:713–716.

National Committee of Pharmacopoeia. Pharmacopoeia of People’s Republic of China (I). 2005 ed. Beijing: Chemical Industry Press; 43,306.

George P, Charles W, eds. Cerebral stereotaxic atlas of rats. 3rd ed. Beijng: People’s Medical Publishing House; 2005:25–34.

Neufeld KL. Nuclear APC. Adv Exp Med Biol 2009;656:13–29.

Pu HH, Jin WF, Li RX, Li B. Preventive and therapeutic effect of Bushen Chinese drugs and alfacalciferol on primary osteoporosis in ovariectomized rats. Fudan Univ J Sci Med (Chin) 2009;36:590–595.

Tian Y, Cai J. Effect of Chinese drugs for tonifying Shen on c-Jun and c-Fos of amygdale in rats. Chin J Integr Med Cardio-/Cerebrovasc Dis (Chin) 2009;7:555–557.

Hanna JH. The STATs on naive iPSC reprogramming. Cell Stem Cell 2010;7:274–276.

Lin HW, Thompson JW, Morris KC, Perez-Pinzon MA. Signal transducers and activators of transcription (STATs)-mediated mitochondrial neuroprotection. Antioxid Redox Signal 2010 Aug 16. [Epub ahead of print]

Skjesol A, Hansen T, Shi CY, Thim HL, Jørgensen JB, et al. Structural and functional studies of STAT1 from Atlantic salmon (Salmo salar). BMC Immunol 2010;30:17.

Xu SB, Liu XH, Li BH, Zhang Y, Yuan J, Yuan Q, et al. DNA methylation regulates constitutive expression of Stat6 regulatory genes SOCS-1 and SHP-1 in colon cancer cells. J Cancer Res Clin Oncol (Chin). 2009;135:1791–1798.

Laurent A, Masse J, Deschamps S, Burel A, Omilli F, Richard-Parpaillon L, et al. Interaction of ZFPIP with PBX1 is crucial for proper expression of neural genetic markers during xenopus development. Dev Growth Differ 2009;51:699–706.

Shimabe M, Goyama S, Watanabe-Okochi N, Yoshimi A, Ichikawa M, Imai Y, et al. Pbx1 is a downstream target of Evi-1 in hematopoietic stem/progenitors and leukemic cells. Oncogene 2009;28:4364–4374.

Fernandez CC, Gudas LJ. The truncated Hoxa1 protein interacts with Hoxa1 and Pbx1 in stem cells. J Cell Biochem 2009;106:427–443.

Coelho DJ, Sims DJ, Ruegg PJ, Minn I, Muench AR, Mitchell PJ, et al. Cell type-specific and sexually dimorphic expression of transcription factor AP-2 in the adult mouse brain. Neuroscience 2005;134:907–919.

Skoblenick KJ, Argintaru N, Xu Y, Dyck BA, Basu D, Tan ML, et al. Role of AP-2alpha transcription factor in the regulation of synapsin II gene expression by dopamine D1 and D2 receptors. J Mol Neurosci 2010;41:267–277.

Fan Z, Yamaza T, Lee JS, Yu J, Wang S, Fan G, et al. BCOR regulates mesenchymal stem cell function by epigenetic mechanisms. Nat Cell Biol 2009;11:1002–1009.

Ding X, Luo C, Zhou J, Zhong Y, Hu X, Zhou F, et al. The interaction of KCTD1 with transcription factor AP-2alpha inhibits its transactivation. J Cell Biochem (Chin) 2009;106:285–295.

Shen ZY, Huang JH, Chen WH. Comparison of gene expression profiles between rats of Shen deficiency syndrome and Shen-yang deficiency syndrome differentiated according to therapeutic efficacy of drugs used. Chin J Integr Tradit West Med (Chin) 2007;27:135–137.

FULL TEXT LINK

More>

Views

776

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Panax notoginseng saponins induced up-regulation, phosphorylation and binding activity of MEK, ERK, AKT, PI-3K protein kinases and GATA transcription factors in hematopoietic cells

Analysis on interrelation between electroacupuncture-induced cumulative analgesic effect and hypothalamic cholinergic activities in chronic neuropathic pain rats

Anti-Obesity effects of Chang-Chul-Eui-Ee-In-Tang (苍术薏苡仁汤) in female rats with diet-induced obesity

Effects of repeated electroacupuncture on β-endorphin and adrencorticotropic hormone levels in the hypothalamus and pituitary in rats with chronic pain and ovariectomy

Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma

Related Author

No data

Related Institution

Department of Oncology, Zhejiang Provincial People’s Hospital

Institution of Hematology Research, The First Affiliated Hospital, Zhejiang Chinese Medical University

Department of Physiology, Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences

Department of Food and Nutrition, Obesity/Diabetes Research Institutes, Hoseo University, Asan, South Korea

Daily Manufacturing Inc., Rockwell

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰