study on the protective effect of the Mixture of Shengmai Powder and Danshen Decoction (生脉散丹参饮合剂) on the myocardium of diabetic cardiomyopathy in the rat model

Qing Ni; Jie Wang; En-qing Li; An-bin Zhao; Bin Yu; Min Wang; Chun-rong Huang

doi:10.1007/s11655-011-0639-9

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study on the protective effect of the Mixture of Shengmai Powder and Danshen Decoction (生脉散丹参饮合剂) on the myocardium of diabetic cardiomyopathy in the rat model

Updated：2021-08-27

- study on the protective effect of the Mixture of Shengmai Powder and Danshen Decoction (生脉散丹参饮合剂) on the myocardium of diabetic cardiomyopathy in the rat model
- Chinese Journal of Integrative Medicine Vol. 17, Issue 2, Pages: 116-125(2011)
- Affiliations：
  
  1. Department of Endocrinology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences,Beijing,China
  2. School of Medicine, Jinan University,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the First Grade of China Postdoctoral Science Foundation (No. 20070410129), the Special Fund by China Postdoctoral Science Foundation (No. 200801166), the Major Project of Beijing Municipal Science and Technology Committee (No. H020920010330) and the Subject of Science and Technology Plan of Beijng Municipal Science and Technology Committee (No. D08050703020802)
- DOI：10.1007/s11655-011-0639-9
  CLC：
- Published：2011，
  
  Published Online：9 March 2011，
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Ni, Q., Wang, J., Li, Eq. et al. study on the protective effect of the Mixture of Shengmai Powder and Danshen Decoction (生脉散丹参饮合剂) on the myocardium of diabetic cardiomyopathy in the rat model., Chin. J. Integr. Med. 17, 116–125 (2011). https://doi.org/10.1007/s11655-011-0639-9

Qing Ni, Jie Wang, En-qing Li, et al. study on the protective effect of the Mixture of Shengmai Powder and Danshen Decoction (生脉散丹参饮合剂) on the myocardium of diabetic cardiomyopathy in the rat model. [J]. Chinese Journal of Integrative Medicine 17(2):116-125(2011)
Ni, Q., Wang, J., Li, Eq. et al. study on the protective effect of the Mixture of Shengmai Powder and Danshen Decoction (生脉散丹参饮合剂) on the myocardium of diabetic cardiomyopathy in the rat model., Chin. J. Integr. Med. 17, 116–125 (2011). https://doi.org/10.1007/s11655-011-0639-9 DOI：

Qing Ni, Jie Wang, En-qing Li, et al. study on the protective effect of the Mixture of Shengmai Powder and Danshen Decoction (生脉散丹参饮合剂) on the myocardium of diabetic cardiomyopathy in the rat model. [J]. Chinese Journal of Integrative Medicine 17(2):116-125(2011) DOI： 10.1007/s11655-011-0639-9.

摘要

To study the protective effect of the Mixture of Shengmai Powder and Danshen Decoction (生脉散丹参饮合剂

abbreviated as the Mixture) in the rat model with type 2 diabetic cardiomyopathy in the rat model with type 2 diabetic cardiomyopathy

abbreviated as the Mixture) in the rat model with type 2 diabetic cardiomyopathy (DCM). Forty-two SD rats with DCM model

established by the combination of insulin resistance by a high-fat diet with the damage of pancreatic islet β cells by intraperitoneal injection of high dose streptozotocin (50 mg/kg) once

were evaluated in the damage of the myocardium by electrocardiogram at the end of 12 weeks of grouping and intervention administration; the extent of damage in the myocardial subcellular structure was observed by electron microscopy; the content of myocardial collagen in the left cardiac ventricle was quantified by Masson staining test; the myocardial cell apoptosis was determined by TUNEL; the changes in the mRNA expression levels of thrombospodin-1 (TSP-1) and tribbles homolog 3 (TRB-3) by real-time quantitative PCR

the expression levels of myocardial TSP-1

tumor growth factorβ1 (TGF-β1)

TRB-3

and chymase were detected by immunohistochemistry

and the changes in the expression levels of myocardial TSP-1

active-TGF-β1 (A-TGF-β1) and latent-TGF-β1 (L-TGF-β1) protein were tested by Western blotting. Compared with the control group

the myocardial tissue was less damaged

and the extent of damage in the myocardial subcellular structure was less; the collagen fiber content and the cell apoptosis were reduced; the expression levels of TSP-1mRNA and TRB-3 mRNA

the expression levels of myocardial TSP-1

TGF-β1

TRB-3

and chymase

as well as the average expression levels of the myocardial TSP-1

A-TGFβ1

and L-TGF-β1 protein were decreased in the Mixture group. The Mixture of Shengmai Powder and Danshen Decoction could inhibit the process of myocardial fibrosis in the rat myocardium of DCM through multiple pathways and significantly delay the genesis and progress of DCM in hyperglycemic rats.

Abstract

To study the protective effect of the Mixture of Shengmai Powder and Danshen Decoction (生脉散丹参饮合剂

abbreviated as the Mixture) in the rat model with type 2 diabetic cardiomyopathy in the rat model with type 2 diabetic cardiomyopathy

abbreviated as the Mixture) in the rat model with type 2 diabetic cardiomyopathy (DCM). Forty-two SD rats with DCM model

established by the combination of insulin resistance by a high-fat diet with the damage of pancreatic islet β cells by intraperitoneal injection of high dose streptozotocin (50 mg/kg) once

the expression levels of myocardial TSP-1

tumor growth factorβ1 (TGF-β1)

TRB-3

and chymase were detected by immunohistochemistry

and the changes in the expression levels of myocardial TSP-1

active-TGF-β1 (A-TGF-β1) and latent-TGF-β1 (L-TGF-β1) protein were tested by Western blotting. Compared with the control group

the myocardial tissue was less damaged

and the extent of damage in the myocardial subcellular structure was less; the collagen fiber content and the cell apoptosis were reduced; the expression levels of TSP-1mRNA and TRB-3 mRNA

the expression levels of myocardial TSP-1

TGF-β1

TRB-3

and chymase

as well as the average expression levels of the myocardial TSP-1

A-TGFβ1

关键词

Mixture of Shengmai Powder and Danshen Decoctiondiabetic cardiomyopathycollagen fiberapoptosisthrombospodin-1tumor growth factor β1tribbles homolog 3chymase

Keywords

Mixture of Shengmai Powder and Danshen Decoctiondiabetic cardiomyopathycollagen fiberapoptosisthrombospodin-1tumor growth factor β1tribbles homolog 3chymase

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