In vivo and in vitro antiviral effects of berberine on influenza virus

Ying Wu; Ji-qian Li; Ye-ji Kim; Jun Wu; Qian Wang; Yu Hao

doi:10.1007/s11655-011-0640-3

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In vivo and in vitro antiviral effects of berberine on influenza virus

Updated：2021-08-27

- In vivo and in vitro antiviral effects of berberine on influenza virus
- Chinese Journal of Integrative Medicine Vol. 17, Issue 6, Pages: 444-452(2011)
- Affiliations：
  
  Key Laboratory of Chinese Medicine on Viral Diseases, Ministry of Education, Microbiology and Immunology Department, Beijing University of Chinese Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30772871)
- DOI：10.1007/s11655-011-0640-3
  CLC：
- Published：2011，
  
  Published Online：10 June 2011，
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Wu, Y., Li, Jq., Kim, Yj. et al. In vivo and in vitro antiviral effects of berberine on influenza virus., Chin. J. Integr. Med. 17, 444–452 (2011). https://doi.org/10.1007/s11655-011-0640-3

Ying Wu, Ji-qian Li, Ye-ji Kim, et al. In vivo and in vitro antiviral effects of berberine on influenza virus. [J]. Chinese Journal of Integrative Medicine 17(6):444-452(2011)
Wu, Y., Li, Jq., Kim, Yj. et al. In vivo and in vitro antiviral effects of berberine on influenza virus., Chin. J. Integr. Med. 17, 444–452 (2011). https://doi.org/10.1007/s11655-011-0640-3 DOI：

Ying Wu, Ji-qian Li, Ye-ji Kim, et al. In vivo and in vitro antiviral effects of berberine on influenza virus. [J]. Chinese Journal of Integrative Medicine 17(6):444-452(2011) DOI： 10.1007/s11655-011-0640-3.

摘要

To explore the potential effects of berberine on influenza virus infection both in vitro and in vivo. In vitro anti-influenza virus assays were performed by cytopathogenic effect and neuraminidase assays in Madin Darby canine kidney cells. In vivo anti-influenza virus assays were performed on the viral pneumonia model of mice. The numbers of mice that died within day 2 to day 14 postinfection were recorded to calculate the mortality. On days 2

and 6

the viral titers in the lungs were determined by hemagglutination assay; hematoxylin/eosin staining was used to assess the pathogenic changes of lung tissues; the concentrations of tumor necrosis factor-alpha (TNF-α) and monocyte specific chemoattractant molecule (MCP-1) were measured by radio immunoassay or enzyme-linked immunosorbent assay; the concentrations of nitric oxide (NO) and inducible nitric oxide synthetase (iNOS) were detected by colorimetric method; reverse transcription polymerase chain reaction was used to detect the mRNA level of TNF-α and MCP-1. Berberine showed inhibitory effects on cytopathogenic effects and neuraminidase activity of virus

with the therapeutic index 9.69. In vivo

berberine decreased mice mortality from 90% to 55%

reduced virus titers in the lungs on day 2 postinfection (P<0.05). The lung histology scores were 1.50±0.67

4.50±1.00

and 5.50±1.00 in the berberine group on days 2

and 6

respectively

which were significantly reduced compared to 2.17±0.22

6.83±0.44

and 8.50±0.33 in the infected group (P<0.05). The productions of NO and iNOS were repressed by berberine compared with those in the infected group (P<0.01). The transcription and expression of TNF-α were inhibited by berberine on day 4 (P<0.01) and day 6 (P<0.05)

and those of MCP-1 were inhibited on day 6 (P<0.01) compared with the infected group. Berberine exhibited antiviral effects on the influenza virus both in vitro and in vivo. The possible therapeutic mechanism of berberine on influenza-induced viral pneumonia might be inhibiting the virus infection

as well as improving the pathogenic changes by repressing inflammatory substances release.

Abstract

and 6

with the therapeutic index 9.69. In vivo

berberine decreased mice mortality from 90% to 55%

reduced virus titers in the lungs on day 2 postinfection (P<0.05). The lung histology scores were 1.50±0.67

4.50±1.00

and 5.50±1.00 in the berberine group on days 2

and 6

respectively

which were significantly reduced compared to 2.17±0.22

6.83±0.44

as well as improving the pathogenic changes by repressing inflammatory substances release.

关键词

in vivoin vitroantiviral effectsBerberineinfluenza virus

Keywords

in vivoin vitroantiviral effectsBerberineinfluenza virus

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Department of Biochemistry, School of Medicine, Jinan University

Institute of Integrative Medicine, Jinan University

Department of Pathophysiology, Key Laboratory of State Administration of Traditional Chinese Medicine, School of Medicine, Jinan University

Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Molecular Nuclear Medicine

Guangdong Province Integrative Medicine Hospital

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