Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums

Xian-pei Heng; Ke-ji Chen; Zhen-feng Hong; Wei-dong He; Ke-dan Chu; Wen-lie Chen; Hai-xia Zheng; Liu-qing Yang; Ling Chen; Fang Guo

doi:10.1007/s11655-011-0646-X

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Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums

Updated：2021-08-27

- Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums
- Chinese Journal of Integrative Medicine Vol. 17, Issue 2, Pages: 126-133(2011)
- Affiliations：
  
  1. Department of Endocrinology, People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. China Academy of Chinese Medical Sciences,Beijing,China
  3. Fujian Institute of Integrative Chinese and Western Medicine,Fuzhou,China
  4. Fujian University of Traditional Chinese Medicine,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the China Postdoctoral Science Foundation (No. 20070410128), CHEN Ke-ji Development Fund of Integrative Medicine (No. CKJ2006001) and Science Foundation of Health Department, Fujian Province (No. WZY0618)
- DOI：10.1007/s11655-011-0646-X
  CLC：
- Published：2011，
  
  Published Online：9 March 2011，
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Heng, Xp., Chen, Kj., Hong, Zf. et al. Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums., Chin. J. Integr. Med. 17, 126–133 (2011). https://doi.org/10.1007/s11655-011-0646-X

Xian-pei Heng, Ke-ji Chen, Zhen-feng Hong, et al. Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums. [J]. Chinese Journal of Integrative Medicine 17(2):126-133(2011)
Heng, Xp., Chen, Kj., Hong, Zf. et al. Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums., Chin. J. Integr. Med. 17, 126–133 (2011). https://doi.org/10.1007/s11655-011-0646-X DOI：

Xian-pei Heng, Ke-ji Chen, Zhen-feng Hong, et al. Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums. [J]. Chinese Journal of Integrative Medicine 17(2):126-133(2011) DOI： 10.1007/s11655-011-0646-X.

摘要

To study the effect of anticolchicine cytotoxicity of Dan Gua-Fang (丹瓜方)

a Chinesea Chinese)

a Chinese herbal compound prescription on endothelial cells of vein (ECV304) cultivated in mediums of different glucose concentrations as well as the proliferation of those cells in the same conditions

in order to reveal the value of Dan Gua-Fang in preventing and treating endothelial damage caused by hyperglycemia in diabetes mellitus. The research was designed as three stages. The growing state and morphological changes were observed when ECV304 were cultivated in the culture mediums

which have different glucose concentrations with or without Dan Gua-Fang and at the same time with or without colchicine. (1) Dan Gua-Fang at all concentrations reduced the floating cell population of ECV304 cultivated in hyperglycemia mediums. (2) Dan Gua-Fang at all concentrations and hyperglycemia both had a function of promoting “pseudopod-like” structure formation in cultivated ECV304

but the function was not superimposed in mediums containing both hyperglycemia and Dan Gua-Fang. (3) Colchicine reduced and even vanished the “pseudopod-like” structure of the endotheliocyte apparently cultivated in mediums of hyperglycemia or with Dan Gua-Fang. The “pseudopod-like” structure of the endotheliocyte emerged quickly in Dan Gua-Fang groups after colchicine was removed

but it was not the case in hyperglycemia only without Dan Gua-Fang groups. (4) Dan Gua-Fang reduced the mortality of cells cultivated in mediums containing colchicine. The cell revived to its normal state fast after colchicine was removed. Dan Gua-Fang has the functions of promoting the formation of cytoskeleton and fighting against colchicine cytotoxicity.

Abstract

To study the effect of anticolchicine cytotoxicity of Dan Gua-Fang (丹瓜方)

a Chinesea Chinese)

关键词

Dan Gua-Fangendothelial cellcolchicinediabetes mellitus

Keywords

Dan Gua-Fangendothelial cellcolchicinediabetes mellitus

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