Effect of berberine on expressions of uncoupling protein-2 mRNA and protein in hepatic tissue of non-alcoholic fatty liver disease in rats

Qin-he Yang; Si-ping Hu; Yu-pei Zhang; Wei-ning Xie; Na Li; Gui-yuan Ji; Na-li Qiao; Xiu-feng Lin; Tong-yan Chen; Hai-tao Liu

doi:10.1007/s11655-011-0668-4

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Effect of berberine on expressions of uncoupling protein-2 mRNA and protein in hepatic tissue of non-alcoholic fatty liver disease in rats

Updated：2021-08-27

- Effect of berberine on expressions of uncoupling protein-2 mRNA and protein in hepatic tissue of non-alcoholic fatty liver disease in rats
- Chinese Journal of Integrative Medicine Vol. 17, Issue 3, Pages: 205-211(2011)
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, Medical College, Jinan University,Guangzhou,China
  2. Guangdong Province Integrative Medicine Hospital,Guangdong Province,Foshan,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-011-0668-4
  CLC：
- Published：2011，
  
  Published Online：27 February 2011，
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Yang, Qh., Hu, Sp., Zhang, Yp. et al. Effect of berberine on expressions of uncoupling protein-2 mRNA and protein in hepatic tissue of non-alcoholic fatty liver disease in rats., Chin. J. Integr. Med. 17, 205–211 (2011). https://doi.org/10.1007/s11655-011-0668-4

Qin-he Yang, Si-ping Hu, Yu-pei Zhang, et al. Effect of berberine on expressions of uncoupling protein-2 mRNA and protein in hepatic tissue of non-alcoholic fatty liver disease in rats. [J]. Chinese Journal of Integrative Medicine 17(3):205-211(2011)
Yang, Qh., Hu, Sp., Zhang, Yp. et al. Effect of berberine on expressions of uncoupling protein-2 mRNA and protein in hepatic tissue of non-alcoholic fatty liver disease in rats., Chin. J. Integr. Med. 17, 205–211 (2011). https://doi.org/10.1007/s11655-011-0668-4 DOI：

Qin-he Yang, Si-ping Hu, Yu-pei Zhang, et al. Effect of berberine on expressions of uncoupling protein-2 mRNA and protein in hepatic tissue of non-alcoholic fatty liver disease in rats. [J]. Chinese Journal of Integrative Medicine 17(3):205-211(2011) DOI： 10.1007/s11655-011-0668-4.

摘要

To observe the effect of berberine on uncoupling protein-2 (UCP2) mRNA and protein expressions in the hepatic tissue of non-alcoholic fatty liver disease (NAFLD) in rats

and to explore the molecular mechanism. To establish the NAFLD rat model; the rats were fed by high fat forage and were randomly divided into four groups: normal group

model group

berberine high-dose group (324 mg/kg)

and berberine low-dose group (162 mg/kg). After treatment for 12 weeks

the expression of UCP2 mRNA in the liver tissue was analyzed by semiquantitative reverse transcription polymerase chain reaction (RT-RTPCR). The expression level of UCP2 protein in the liver tissue was examined by immunohistochemistry. Total PCR). cholesterol (TC)

triglyceride (TG)

high-density lipoprotein cholesterol (HDL-C)

and low-density lipoprotein cholesterol (LDL-C) contents in blood serum

and TG and TC contents in the liver were detected by an automatic biochemical analyzer. The other is to observe the axungia degree of the liver. The expression of UCP2 mRNA and positive cell numbers in the liver tissue were dramatically increased in the model group (P<0.01). Lipid in the serum and hepatic tissues increased significantly

and the liver was fatty. But in the treatment groups

the expression levels of mRNA and UCP2 proteins were significantly down-regulated (P<0.01). Liver steatosis was improved. Berberine can down-regulate the expression levels of UCP2 mRNA and UCP2 proteins of hepatic tissue in NAFLD rats. It can promote the recovery of hepatocyte steatosis and improve lipid metabolism disorder in NAFLD rats. Berberine shows a potential therapeutic effect on NAFLD.

Abstract

To observe the effect of berberine on uncoupling protein-2 (UCP2) mRNA and protein expressions in the hepatic tissue of non-alcoholic fatty liver disease (NAFLD) in rats

and to explore the molecular mechanism. To establish the NAFLD rat model; the rats were fed by high fat forage and were randomly divided into four groups: normal group

model group

berberine high-dose group (324 mg/kg)

and berberine low-dose group (162 mg/kg). After treatment for 12 weeks

triglyceride (TG)

high-density lipoprotein cholesterol (HDL-C)

and low-density lipoprotein cholesterol (LDL-C) contents in blood serum

and the liver was fatty. But in the treatment groups

关键词

non-alcoholic fatty liver diseaseBerberineuncoupling protein-2Experimental Study

Keywords

non-alcoholic fatty liver diseaseBerberineuncoupling protein-2Experimental Study

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