Effects of inotodiol extracts from inonotus obliquus on proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line A549

Xiu-hong Zhong; Li-bo Wang; Dong-zhi Sun

doi:10.1007/s11655-011-0670-x

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Effects of inotodiol extracts from inonotus obliquus on proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line A549

Updated：2021-08-27

- Effects of inotodiol extracts from inonotus obliquus on proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line A549
- Chinese Journal of Integrative Medicine Vol. 17, Issue 3, Pages: 218-223(2011)
- Affiliations：
  
  1. Department of Pathology, Jilin Medical College,Jilin,China
  2. Department of Pathology, Jilin City People’s Hospital,Jilin,China
  3. Department of Pathology and Forensic Medicine, Basic Medical College, Yanbian University Health Science Center, Yanji,Jilin,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-011-0670-x
  CLC：
- Published：2011，
  
  Published Online：27 February 2011，
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Zhong, Xh., Wang, Lb. & Sun, Dz. Effects of inotodiol extracts from inonotus obliquus on proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line A549., Chin. J. Integr. Med. 17, 218–223 (2011). https://doi.org/10.1007/s11655-011-0670-x

Xiu-hong Zhong, Li-bo Wang, Dong-zhi Sun. Effects of inotodiol extracts from inonotus obliquus on proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line A549. [J]. Chinese Journal of Integrative Medicine 17(3):218-223(2011)
Zhong, Xh., Wang, Lb. & Sun, Dz. Effects of inotodiol extracts from inonotus obliquus on proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line A549., Chin. J. Integr. Med. 17, 218–223 (2011). https://doi.org/10.1007/s11655-011-0670-x DOI：

Xiu-hong Zhong, Li-bo Wang, Dong-zhi Sun. Effects of inotodiol extracts from inonotus obliquus on proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line A549. [J]. Chinese Journal of Integrative Medicine 17(3):218-223(2011) DOI： 10.1007/s11655-011-0670-x.

摘要

To observe the proliferation inhibition

apoptosis

and cell proliferation cycle of human lung carcinoma cell line A549 treated with Inotodiol extracts from Inonotus obliquus and explore the possibility of Inotodiol extracts from Inonotus obliquus as a new tumor chemopreventive drug. Human lung cancer cell line A549 was treated with different concentrations of Inotodiol

the effects of Inotodiol on cell apoptosis

the expression of Ki-67

Bcl-2

Bax

and p53 and cell cycle were detected by TUNEL assay

immunohistochemistry

and flow cytometry assay respectively. Inotodiol extracts had antiproliferation effect on human lung carcinoma cell line A549. The expression of Ki-67 decreased with the increase of Inotodiol concentration and exposure time (P<0.05)

in a dose-dependent and time-dependent manner. The typical characteristics of the apoptosis of A549 cells treated with Inotodiol were observed

and the apoptotic rate of A549 cell at 48 h was the highest by TUNEL assay. Inotodiol arrested A549 cells in the S phase

and apoptotic peak was observed by flow cytometry. Immunocytochemistry indicated that the expression of Bcl-2 protein decreased

while the expression of p53 and Bax proteins increased in A549 cells treated with Inotodiol

compared with the control cells (P<0.05). Inotodiol can inhibit proliferation and induce the apoptosis of A549 cells

and its molecular mechanism may be associated with the up-regulating expression of p53 and bax proteins and down-regulating expression of Bcl-2 protein

which arrested A549 cells in S phase.

Abstract

To observe the proliferation inhibition

apoptosis

the effects of Inotodiol on cell apoptosis

the expression of Ki-67

Bcl-2

Bax

and p53 and cell cycle were detected by TUNEL assay

immunohistochemistry

in a dose-dependent and time-dependent manner. The typical characteristics of the apoptosis of A549 cells treated with Inotodiol were observed

and the apoptotic rate of A549 cell at 48 h was the highest by TUNEL assay. Inotodiol arrested A549 cells in the S phase

and apoptotic peak was observed by flow cytometry. Immunocytochemistry indicated that the expression of Bcl-2 protein decreased

while the expression of p53 and Bax proteins increased in A549 cells treated with Inotodiol

compared with the control cells (P<0.05). Inotodiol can inhibit proliferation and induce the apoptosis of A549 cells

and its molecular mechanism may be associated with the up-regulating expression of p53 and bax proteins and down-regulating expression of Bcl-2 protein

which arrested A549 cells in S phase.

关键词

Inonotus obliquusA549 apoptotic genecell cyclecell proliferationKi-67

Keywords

Inonotus obliquusA549 apoptotic genecell cyclecell proliferationKi-67

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Department of Integrated Traditional Chinese and Western Medicine, Second Affiliated Hospital of Medical School of Xi’an Jiaotong University

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