Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells

Fu-xing Li; Shu-sheng Li

doi:10.1007/s11655-011-0700-8

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Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells

Updated：2021-08-27

- Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells
- Chinese Journal of Integrative Medicine Vol. 18, Issue 5, Pages: 391-394(2012)
- Affiliations：
  
  1. Department of Emergency, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan,China
  2. Department of Emergency, the Second Affiliated Hospital to Nanchang University,Nanchang,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-011-0700-8
  CLC：
- Published：2012，
  
  Published Online：26 April 2011，
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Li, Fx., Li, Ss. Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells., Chin. J. Integr. Med. 18, 391–394 (2012). https://doi.org/10.1007/s11655-011-0700-8

Fu-xing Li, Shu-sheng Li. Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells. [J]. Chinese Journal of Integrative Medicine 18(5):391-394(2012)
Li, Fx., Li, Ss. Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells., Chin. J. Integr. Med. 18, 391–394 (2012). https://doi.org/10.1007/s11655-011-0700-8 DOI：

Fu-xing Li, Shu-sheng Li. Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells. [J]. Chinese Journal of Integrative Medicine 18(5):391-394(2012) DOI： 10.1007/s11655-011-0700-8.

摘要

To observe the effect of andrographolide on the activation of mitogen-activated protein kinases (MAPKs) and expression of nuclear factor-κB (NF-κB) in macrophage foam cells. The mouse peritoneal macrophages were cultured in the media in the presence of oxidized low-density lipoprotein (ox-LDL)

ox-LDL+andrographolide

or neither (control). The phosphorylation of MAPK molecules (p38MAPK

JNK

ERK1/2) and the expressions of NK-κB p65 were examined by Western blot. As compared with cells in the control group

the expressions of phospho-p38 and NF-κB p65 were increased in the cells cultured with either ox-LDL or ox-LDL+andrographolide (P<0.01)

but attenuated significantly in the presence of ox-LDL+ andrographolide when compared with ox-LDL (P<0.05). The phospho-JNK increased in the presence of either ox-LDL or ox-LDL+andrographolide when compared with control cells (P<0.01)

but no significant difference existed between ox-LDL and ox-LDL+andrographolide (P>0.05). The expression of phospho-ERK1/2 was increased in the presence of ox-LDL compared with the control cells (P<0.01)

but no significant differences existed between the cells cultured in the presence of ox-LDL+andrographolide and the control medium (P>0.05). Andrographolide could inhibit the activation of ERK1/2

p38MAPK and NK-κB induced by ox-LDL in macrophage foam cells

which might be one of its mechanisms in preventing atherosclerosis.

Abstract

ox-LDL+andrographolide

or neither (control). The phosphorylation of MAPK molecules (p38MAPK

JNK

ERK1/2) and the expressions of NK-κB p65 were examined by Western blot. As compared with cells in the control group

the expressions of phospho-p38 and NF-κB p65 were increased in the cells cultured with either ox-LDL or ox-LDL+andrographolide (P<0.01)

but no significant differences existed between the cells cultured in the presence of ox-LDL+andrographolide and the control medium (P>0.05). Andrographolide could inhibit the activation of ERK1/2

p38MAPK and NK-κB induced by ox-LDL in macrophage foam cells

which might be one of its mechanisms in preventing atherosclerosis.

关键词

andrographolidemouse peritoneal macrophage foam cellsmitogen activated protein kinasesenuclear factor-κBatherosclerosis

Keywords

andrographolidemouse peritoneal macrophage foam cellsmitogen activated protein kinasesenuclear factor-κBatherosclerosis

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Institute of Basic Theories of Traditional Chinese Medicine, China Academy of Traditional Chinese Medicine

Department of Oncology, Clifford Hospital of Guangzhou University of Traditional Chinese Medicine

Department of Gynaecology and Obstetrics, Shenzhen Baoan Maternal and Child Health Hospital

Department of Obstetrics and Gynecology, First Affiliated Hospital of Jinan University

Shandong Provincial Corps Hospital of Chinese People’s Armed Police Forces

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