Extract of Paris polyphylla Simth protects cardiomyocytes from anoxia-reoxia injury through inhibition of calcium overload

Peng Li; Jian-hua Fu; Jing-kun Wang; Jun-guo Ren; Jian-xun Liu

doi:10.1007/s11655-011-0704-4

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Extract of Paris polyphylla Simth protects cardiomyocytes from anoxia-reoxia injury through inhibition of calcium overload

Updated：2021-08-27

- Extract of Paris polyphylla Simth protects cardiomyocytes from anoxia-reoxia injury through inhibition of calcium overload
- Chinese Journal of Integrative Medicine Vol. 17, Issue 4, Pages: 283-289(2011)
- Affiliations：
  
  1. The Laboratory Research Center of Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
  2. Yunnan Pharmaceutical Institute,Kunming,China
- Author bio：
- Funds：
  
  Supported by the Major Scientific and Technological Specialized Project for “Significant New Formulation of New Drugs (No. 2009ZX09301, 2009ZX09303-003)”
- DOI：10.1007/s11655-011-0704-4
  CLC：
- Published：2011，
  
  Published Online：21 April 2011，
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Li, P., Fu, Jh., Wang, Jk. et al. Extract of Paris polyphylla Simth protects cardiomyocytes from anoxia-reoxia injury through inhibition of calcium overload., Chin. J. Integr. Med. 17, 283–289 (2011). https://doi.org/10.1007/s11655-011-0704-4

Peng Li, Jian-hua Fu, Jing-kun Wang, et al. Extract of Paris polyphylla Simth protects cardiomyocytes from anoxia-reoxia injury through inhibition of calcium overload. [J]. Chinese Journal of Integrative Medicine 17(4):283-289(2011)
Li, P., Fu, Jh., Wang, Jk. et al. Extract of Paris polyphylla Simth protects cardiomyocytes from anoxia-reoxia injury through inhibition of calcium overload., Chin. J. Integr. Med. 17, 283–289 (2011). https://doi.org/10.1007/s11655-011-0704-4 DOI：

Peng Li, Jian-hua Fu, Jing-kun Wang, et al. Extract of Paris polyphylla Simth protects cardiomyocytes from anoxia-reoxia injury through inhibition of calcium overload. [J]. Chinese Journal of Integrative Medicine 17(4):283-289(2011) DOI： 10.1007/s11655-011-0704-4.

摘要

To assess any direct effect of extract of Paris polyphylla Simth (EPPS)

a Chinese plant

on a cardiomyocyte subject to ischemia-reperfusion injury and to further elucidate its protective effect against myocardium ischemia on the cellular level. Neonatal rat cardiomyocytes were isolated and subjected to an anoxia-reoxia injury simulating the ischemia-reperfusion injury in vivo in the presence or absence of EPPS or diltizem

a positive control. The lactate dehydrogenase (LDH) activities in culture supernatants and cell viabilities were analyzed using the enzymatic reaction kinetics monitoring-method and MTT method

respectively. Free intracellular calcium concentrations and activities of Na+-K+ ATPase and Ca2+ ATPase in cells were also measured with laser confocal microscopy and the inorganic phosphorus-transformation method

respectively. In cardiomyocytes subject to anoxia-reoxia injury

EPPS at 50–400 mg/L showed a concentration-dependent inhibition on LDH leakage and maintenance of cell viability

and the effect was significant at 275 and 400 mg/L (both P<0.01). In addition

EPPS at 275 and 400 mg/L significantly inhibited the increase in intracellular free calcium (both P<0.01) as well as decreased the activities of Na+-K+ ATPase and Ca2+ ATPase (P<0.01

P<0.05). EPPS prevents anoxia-reoxia injury in neonatal rat cardiomyocytes in vitro by preservation of Na+-K+ ATPase and Ca2+ ATPase activities and inhibition of calcium overload. The direct protective effect on cardiomyocytes may be one of the key mechanisms that underlie the potential therapeutic benefit of EPPS against myocardium ischemia.

Abstract

To assess any direct effect of extract of Paris polyphylla Simth (EPPS)

a Chinese plant

a positive control. The lactate dehydrogenase (LDH) activities in culture supernatants and cell viabilities were analyzed using the enzymatic reaction kinetics monitoring-method and MTT method

respectively. In cardiomyocytes subject to anoxia-reoxia injury

EPPS at 50–400 mg/L showed a concentration-dependent inhibition on LDH leakage and maintenance of cell viability

and the effect was significant at 275 and 400 mg/L (both P<0.01). In addition

EPPS at 275 and 400 mg/L significantly inhibited the increase in intracellular free calcium (both P<0.01) as well as decreased the activities of Na+-K+ ATPase and Ca2+ ATPase (P<0.01

关键词

Paris polyphylla Simthcardiomyocyteanoxia-reoxia injurycalcium-overloadischemia-reperfusion injury

Keywords

Paris polyphylla Simthcardiomyocyteanoxia-reoxia injurycalcium-overloadischemia-reperfusion injury

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Related Institution

Gansu College of Traditional Chinese Medicine

Graduate Institute of Integration Chinese and Western Medicine, Lanzhou University

Center for Human Genome Research, College of Life Science and Technology, Huazhong University of Science andTechnology

Department of Cardiology, 1st Hospital, Zhengzhou University

Department of Internal Medicine, Hubei Hospital of Traditional Chinese Medicine, Hubei College of Traditional Chinese Medicine

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