Blocking effect of salvianolic acid a on calcium channels in isolated rat ventricular myocytes

Bao Wang; Jian-xun Liu; Hong-xu Meng; Cheng-ren Lin

doi:10.1007/s11655-011-0707-1

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Blocking effect of salvianolic acid a on calcium channels in isolated rat ventricular myocytes

Updated：2021-08-27

- Blocking effect of salvianolic acid a on calcium channels in isolated rat ventricular myocytes
- Chinese Journal of Integrative Medicine Vol. 18, Issue 5, Pages: 366-370(2012)
- Affiliations：
  
  The Laboratory Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Key Project of National Science Foundation of China (No. 30830118) and the National Key New Drug Project (No. 2009ZX09301-005 and No. 2009ZX09303-003).
- DOI：10.1007/s11655-011-0707-1
  CLC：
- Published：2012，
  
  Published Online：26 April 2011，
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Wang, B., Liu, Jx., Meng, Hx. et al. Blocking effect of salvianolic acid a on calcium channels in isolated rat ventricular myocytes., Chin. J. Integr. Med. 18, 366–370 (2012). https://doi.org/10.1007/s11655-011-0707-1

Bao Wang, Jian-xun Liu, Hong-xu Meng, et al. Blocking effect of salvianolic acid a on calcium channels in isolated rat ventricular myocytes. [J]. Chinese Journal of Integrative Medicine 18(5):366-370(2012)
Wang, B., Liu, Jx., Meng, Hx. et al. Blocking effect of salvianolic acid a on calcium channels in isolated rat ventricular myocytes., Chin. J. Integr. Med. 18, 366–370 (2012). https://doi.org/10.1007/s11655-011-0707-1 DOI：

Bao Wang, Jian-xun Liu, Hong-xu Meng, et al. Blocking effect of salvianolic acid a on calcium channels in isolated rat ventricular myocytes. [J]. Chinese Journal of Integrative Medicine 18(5):366-370(2012) DOI： 10.1007/s11655-011-0707-1.

摘要

To study the effect of salvianolic acid A (SAA) on L-type calcium current (I-CaL) in isolated ventricular myocytes of Sprague-Dawley rats. SAA powder was dissolved in normal Tyrode’s solution to reach the concentrations of 1

100

and 1000 μmol/L. The traditional whole-cell patch-clamp recording technique was employed to evaluate the effects of SAA on I-CaL in single ventricular myocytes which were prepared by Langendorff perfusion apparatus from Sprague-Dawley rats. SAA (1

100

and 1000 μmol/L) inhibited I-CaL peak value by 16.23%±1.3% (n=6

P<0.05)

22.9%±3.6% (n=6

P<0.05)

53.4%±3.0% (n=8

P<0.01)

and 62.26%±2.9% (n=6

P<0.01)

respectively. SAA reversibly inhibited I-CaL in a dose-dependent manner and with a half-blocking concentration (IC50) of 38.3 μmol/L. SAA at 100 μmol/L elevated the I-V curve obviously

and shifted the half-active voltage (V0.5) from (−15.78±0.86) mV to (−11.24 ±0.77) mV (n=6

P<0.05) and the slope (K) from 5.33±0.74 to 4.35±0.74 (n=6

P>0.05). However

it did not alter the shapes of I–V curve

steady-state inactivation curve

or recovery from inactivation curve. SAA inhibited I-CaL in a dose-dependent manner. It shifted the steady-state activation curve to a more positive voltage

which indicated that the drug affected the activated state of calcium channels

and suggested that the Ca2+ antagonistic effect of SAA be beneficial in the treatment of myocardial ischemia reperfusion injury.

Abstract

100

and 1000 μmol/L) inhibited I-CaL peak value by 16.23%±1.3% (n=6

P<0.05)

22.9%±3.6% (n=6

P<0.05)

53.4%±3.0% (n=8

P<0.01)

and 62.26%±2.9% (n=6

P<0.01)

respectively. SAA reversibly inhibited I-CaL in a dose-dependent manner and with a half-blocking concentration (IC50) of 38.3 μmol/L. SAA at 100 μmol/L elevated the I-V curve obviously

and shifted the half-active voltage (V0.5) from (−15.78±0.86) mV to (−11.24 ±0.77) mV (n=6

P<0.05) and the slope (K) from 5.33±0.74 to 4.35±0.74 (n=6

P>0.05). However

it did not alter the shapes of I–V curve

steady-state inactivation curve

or recovery from inactivation curve. SAA inhibited I-CaL in a dose-dependent manner. It shifted the steady-state activation curve to a more positive voltage

which indicated that the drug affected the activated state of calcium channels

and suggested that the Ca2+ antagonistic effect of SAA be beneficial in the treatment of myocardial ischemia reperfusion injury.

关键词

salvianolic acid AVentricular Myocytespatch-clamp techniqueL-type calcium channels

Keywords

salvianolic acid AVentricular Myocytespatch-clamp techniqueL-type calcium channels

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