Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate

Xin Tong; Hui Sun; Guang-li Yan; Wei Dong; Wen-jun Sun; Yang Yu; Ping Wang; Ying Han; Xi-jun Wang

doi:10.1007/s11655-011-0728-9

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Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate

Updated：2021-08-27

- Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate
- Chinese Journal of Integrative Medicine Vol. 17, Issue 5, Pages: 369-375(2011)
- Affiliations：
  
  Heilongjiang University of Traditional Chinese Medicine, Haerbin,Heilongjiang Province,China
- Author bio：
- Funds：
  
  Supported by the Key Items of the National Program for Basic Sciences Research and Development (“973” Plan No. 2005CB523406)
- DOI：10.1007/s11655-011-0728-9
  CLC：
- Published：2011，
  
  Published Online：25 May 2011，
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Tong, X., Sun, H., Yan, Gl. et al. Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate., Chin. J. Integr. Med. 17, 369–375 (2011). https://doi.org/10.1007/s11655-011-0728-9

Xin Tong, Hui Sun, Guang-li Yan, et al. Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate. [J]. Chinese Journal of Integrative Medicine 17(5):369-375(2011)
Tong, X., Sun, H., Yan, Gl. et al. Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate., Chin. J. Integr. Med. 17, 369–375 (2011). https://doi.org/10.1007/s11655-011-0728-9 DOI：

Xin Tong, Hui Sun, Guang-li Yan, et al. Evaluation study on urine metabolomics in Yinhuang (阴黄) rat model induced by triplet factors of rhubarb, ethanol, and α-nephthylisothiolyanate. [J]. Chinese Journal of Integrative Medicine 17(5):369-375(2011) DOI： 10.1007/s11655-011-0728-9.

摘要

To ascertain the biomarkers capable to characterize the animal composite model of Chinese medicine (CM) Yinhuang (阴黄) syndrome induced by triplet factors of rhubarb

ethanol

and α-nephthylisothiolyanate (abbreviated as R

and A below) through metabolomic study and to evaluate the established model by means of studying the sources of markers based on the changes of metabolites produced from various combinations of the three modeling drugs. Eighty Wistar rats allocated equally in eight groups (A–H) were treated with saline

R+E+A

R+E

R+A

and E+A

respectively. Rats’ 12 h urine in the 14 successive experimental days were collected separately using metabolic cages and analyzed by ultra peformance liquid chromatograph/time of flight mass spectrometer (UPLC/TOF-MS) to create the metabolic contour graph of urine in different groups for identifying the differences between them. The similarities and differences of metabolic network among various groups were represented from microcosmic viewpoint by pattern recognition method (principal component analysis). Controlled by group A

the landing points in principal component map of various groups were apparently assorted

especially obvious on the 14th day; 19 biomarkers

which capable to represent the genesis and development process of the Yinhuang syndrome in the triplet factors-induced rat model

were identified. Metabolomic method is successfully used in evaluating the animal model of CM syndrome. Furthermore

according to the holistic view and substance changes in vivo

the influences of disease on organism were comprehensively analyzed

and the pathogenic mechanism of CM Yinhuang syndrome was explored at the level of metabolomics in vivo as well.

Abstract

To ascertain the biomarkers capable to characterize the animal composite model of Chinese medicine (CM) Yinhuang (阴黄) syndrome induced by triplet factors of rhubarb

ethanol

and α-nephthylisothiolyanate (abbreviated as R

R+E+A

R+E

R+A

and E+A

the landing points in principal component map of various groups were apparently assorted

especially obvious on the 14th day; 19 biomarkers

which capable to represent the genesis and development process of the Yinhuang syndrome in the triplet factors-induced rat model

were identified. Metabolomic method is successfully used in evaluating the animal model of CM syndrome. Furthermore

according to the holistic view and substance changes in vivo

the influences of disease on organism were comprehensively analyzed

and the pathogenic mechanism of CM Yinhuang syndrome was explored at the level of metabolomics in vivo as well.

关键词

rhubarbethanolα-nephthylisothiolyanateYinhuang syndromeanimal modelmetabolomics

Keywords

rhubarbethanolα-nephthylisothiolyanateYinhuang syndromeanimal modelmetabolomics

references

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