Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes

Ming-xue Zhang; Yu-mei Zhang; Jun Esther; Wei He; Hong-hua Che; Ping Gu

doi:10.1007/s11655-011-0729-8

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Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes

Updated：2021-08-27

- Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes
- Chinese Journal of Integrative Medicine Vol. 17, Issue 5, Pages: 376-380(2011)
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang,China
  2. School of Oriental Medicine, Health Center, Life University, Marietta,USA
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 30873399);Doctoral Funds from Ministry of Education (No. 20070162002);and Excellent Talents in Universities Support Plan of Liaoning Province
- DOI：10.1007/s11655-011-0729-8
  CLC：
- Published：2011，
  
  Published Online：25 May 2011，
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Zhang, Mx., Zhang, Ym., Esther, J. et al. Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes., Chin. J. Integr. Med. 17, 376–380 (2011). https://doi.org/10.1007/s11655-011-0729-8

Ming-xue Zhang, Yu-mei Zhang, Jun Esther, et al. Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes. [J]. Chinese Journal of Integrative Medicine 17(5):376-380(2011)
Zhang, Mx., Zhang, Ym., Esther, J. et al. Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes., Chin. J. Integr. Med. 17, 376–380 (2011). https://doi.org/10.1007/s11655-011-0729-8 DOI：

Ming-xue Zhang, Yu-mei Zhang, Jun Esther, et al. Effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie virus B type 3 on the expression of ribosomal protein S20 in rat cardiac myocytes. [J]. Chinese Journal of Integrative Medicine 17(5):376-380(2011) DOI： 10.1007/s11655-011-0729-8.

摘要

To study the effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie B virus type 3 (CVB3) on the expression of ribosomal protein S20 in rat cardiac myocytes

to explore the pathogenesis of myocarditis induced by CVB3 and the mechanism of Yiqi Huoxue Recipe on gene level

and to further investigate whether Yiqi Huoxue Recipe is an effective prescription for CVB3 myocarditis. A modified suppression subtractive hybridization (SSH) was used to isolate differentially expressed genes between the CVB3 infection group and the treatment group with Yiqi Huoxue Recipe. The results were further verified by fluorescence RT-PCR. The results of SSH showed that the gene expression of ribosomal protein S20 in the treatment group was higher than that in the CVB3 infection group (P<0.05)

which agreed with the results of fluorescent RT-PCR. Down-regulation of ribosomal protein S20 mRNA expression might be one of the mechanisms in CVB3 myocarditis

and Yiqi Huoxue Recipe could achieve the treatment of viral myocarditis by regulating the expression of ribosomal protein S20.

Abstract

To study the effects of Yiqi Huoxue Recipe (益气活血方) and Coxsackie B virus type 3 (CVB3) on the expression of ribosomal protein S20 in rat cardiac myocytes

to explore the pathogenesis of myocarditis induced by CVB3 and the mechanism of Yiqi Huoxue Recipe on gene level

which agreed with the results of fluorescent RT-PCR. Down-regulation of ribosomal protein S20 mRNA expression might be one of the mechanisms in CVB3 myocarditis

and Yiqi Huoxue Recipe could achieve the treatment of viral myocarditis by regulating the expression of ribosomal protein S20.

关键词

Viral MyocarditisYiqi Huoxue recipesuppression subtractive hybridizationribosomal protein S20

Keywords

Viral MyocarditisYiqi Huoxue recipesuppression subtractive hybridizationribosomal protein S20

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Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences

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