Experimental study on the suppression of sodium nitroprussiate-induced chondrocyte apoptosis by Tougu Xiaotong Capsule (透骨消痛胶囊)-containing serum

Xi-hai Li; Ming-xia Wu; Hong-zhi Ye; Wen-lie Chen; Jiu-mao Lin; Liang-pu Zheng; Xian-xiang Liu

doi:10.1007/s11655-011-0751-x

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Experimental study on the suppression of sodium nitroprussiate-induced chondrocyte apoptosis by Tougu Xiaotong Capsule (透骨消痛胶囊)-containing serum

Updated：2021-08-27

- Experimental study on the suppression of sodium nitroprussiate-induced chondrocyte apoptosis by Tougu Xiaotong Capsule (透骨消痛胶囊)-containing serum
- Chinese Journal of Integrative Medicine Vol. 17, Issue 6, Pages: 436-443(2011)
- Affiliations：
  
  1. Academy of Integrative Medicine of Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. Fujian Provincial Key Lab for Integrative Medical Study on Senile Diseases,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072826), Major Project Foundation of Fujian Provincial Administration of Science and Technology (No. 2009Y0029), and the Developmental Fund of CHEN Keji Integrative Medicine (No. CKJ2010013)
- DOI：10.1007/s11655-011-0751-x
  CLC：
- Published：2011，
  
  Published Online：10 June 2011，
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Li, Xh., Wu, Mx., Ye, Hz. et al. Experimental study on the suppression of sodium nitroprussiate-induced chondrocyte apoptosis by Tougu Xiaotong Capsule (透骨消痛胶囊)-containing serum., Chin. J. Integr. Med. 17, 436–443 (2011). https://doi.org/10.1007/s11655-011-0751-x

Xi-hai Li, Ming-xia Wu, Hong-zhi Ye, et al. Experimental study on the suppression of sodium nitroprussiate-induced chondrocyte apoptosis by Tougu Xiaotong Capsule (透骨消痛胶囊)-containing serum. [J]. Chinese Journal of Integrative Medicine 17(6):436-443(2011)
Li, Xh., Wu, Mx., Ye, Hz. et al. Experimental study on the suppression of sodium nitroprussiate-induced chondrocyte apoptosis by Tougu Xiaotong Capsule (透骨消痛胶囊)-containing serum., Chin. J. Integr. Med. 17, 436–443 (2011). https://doi.org/10.1007/s11655-011-0751-x DOI：

Xi-hai Li, Ming-xia Wu, Hong-zhi Ye, et al. Experimental study on the suppression of sodium nitroprussiate-induced chondrocyte apoptosis by Tougu Xiaotong Capsule (透骨消痛胶囊)-containing serum. [J]. Chinese Journal of Integrative Medicine 17(6):436-443(2011) DOI： 10.1007/s11655-011-0751-x.

摘要

To study the mechanism of action of Tougu Xiaotong Capsule (透骨消痛胶囊

TGXTC) ex vivo in suppressing chondrocyte (CD) apoptosis induced by sodium nitroprussiate (SNP). Thirty New Zealand rabbits

2 months old

were randomized by lottery into five groups

six in each: the blank group treated with saline

the positive control group treated with Zhuanggu Guanjie Pill (壮骨关节丸

70 mg/kg)

and the three experimental groups

EGA

EGB

and EGC

treated with low dose (35 mg/kg)

moderate dose (70 mg/kg)

and high dose (140 mg/kg) of TGXTC

respectively. All treatments were administered via gastrogavage twice a day for 3 days. Arterial blood was collected from the abdominal aorta and drug or drug metabolites-containing serum was prepared. CDs obtained from knee joints of 16 four-week-old New Zealand rabbits were cultured to the third passage and confirmed by toluidine blue staining. SNP of various final concentrations (0

0.5

1.0

and 2.0 mmol/L) was used to induce CD apoptosis

and the dosage-effect relationship of SNP in inducing CD apoptosis was determined. Serum samples from the blank

control

and three dosages of TGXTC-treated rabbits were tested in the CD culture in the presence of SNP. Cell apoptosis was determined by Hoechst 33342 staining

viability of CDs was quantified by MTT

CD apoptosis rate was determined by annexin V-FITC/PI staining

levels of p53 and Bcl-2 mRNA expression in CDs were determined with RT-PCR

and contents of caspase-3 and caspase-9 proteins were determined by colorimetry. CD apoptosis was induced by SNP at all concentrations tested and in a dose-dependent manner. The SNP concentration of 1 mmol/L and treatment duration of 24 h appeared to be optimal and were selected for the study. Serum samples from the positive control rabbits and from the two higher doses of TGXTC-treated rabbits showed reduction of SNP-induced CD apoptosis

decrease in p53 mRNA expression

inhibition of catalytic activities of caspase-3 and caspase-9

and increase in Bcl-2 mRNA expression when compared with the serum from the blank group (P<0.05). TGXTC-containing sera antagonized SNP-induced CD apoptosis and the molecular basis for the action was associated with up-regulation of Bcl-2

down-regulation of p53 expression

and inhibition of caspase-3 and caspase-9 catalytic activities.

Abstract

To study the mechanism of action of Tougu Xiaotong Capsule (透骨消痛胶囊

TGXTC) ex vivo in suppressing chondrocyte (CD) apoptosis induced by sodium nitroprussiate (SNP). Thirty New Zealand rabbits

2 months old

were randomized by lottery into five groups

six in each: the blank group treated with saline

the positive control group treated with Zhuanggu Guanjie Pill (壮骨关节丸

70 mg/kg)

and the three experimental groups

EGA

EGB

and EGC

treated with low dose (35 mg/kg)

moderate dose (70 mg/kg)

and high dose (140 mg/kg) of TGXTC

0.5

1.0

and 2.0 mmol/L) was used to induce CD apoptosis

and the dosage-effect relationship of SNP in inducing CD apoptosis was determined. Serum samples from the blank

control

and three dosages of TGXTC-treated rabbits were tested in the CD culture in the presence of SNP. Cell apoptosis was determined by Hoechst 33342 staining

viability of CDs was quantified by MTT

CD apoptosis rate was determined by annexin V-FITC/PI staining

levels of p53 and Bcl-2 mRNA expression in CDs were determined with RT-PCR

decrease in p53 mRNA expression

inhibition of catalytic activities of caspase-3 and caspase-9

down-regulation of p53 expression

and inhibition of caspase-3 and caspase-9 catalytic activities.

关键词

osteoarthritisdrug-containing serumchondrocytecell apoptosisTougu Xiaotong Capsule

Keywords

osteoarthritisdrug-containing serumchondrocytecell apoptosisTougu Xiaotong Capsule

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Institute of Chinese Materia Medica of Shanghai University of Traditional Chinese Medicine

Research Institute of Orthopaedics, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Wangjing Hospital, China Academy of Chinese Medical Sciences

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Fujian University of Traditional Chinese Medicine

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