Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats

Zhi-kui Chen; Li-wu Lin; Jing Cai; Fa-duan Yang; Hua-jing Cai; En-sheng Xue; Jing Huang; Hong-fen Wei; Xiu-juan Zhang

doi:10.1007/s11655-011-0757-4

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Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats

Updated：2021-08-27

- Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats
- Chinese Journal of Integrative Medicine Vol. 19, Issue 1, Pages: 48-53(2013)
- Affiliations：
  
  1. Ultrasonic Department, Affiliated Union Hospital of Fujian Medical University,Fuzhou,China
  2. Department of Pathology, Affiliated Xiehe Hospital of Fujian Medical University,Fuzhou,China
  3. Integrative Medical Institute of Fujian University of Traditional Chinese Medicine,Fuzhou,China
  4. Department of Pharmacology, College of Pharmacy, Fujian Medical University,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the Fujian Provincial Funds of Natural Sciences for Creative Items of Youth (No. 2009J05066)
- DOI：10.1007/s11655-011-0757-4
  CLC：
- Published：2013，
  
  Published Online：29 June 2011，
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Chen, Zk., Lin, Lw., Cai, J. et al. Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats., Chin. J. Integr. Med. 19, 48–53 (2013). https://doi.org/10.1007/s11655-011-0757-4

Zhi-kui Chen, Li-wu Lin, Jing Cai, et al. Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats. [J]. Chinese Journal of Integrative Medicine 19(1):48-53(2013)
Chen, Zk., Lin, Lw., Cai, J. et al. Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats., Chin. J. Integr. Med. 19, 48–53 (2013). https://doi.org/10.1007/s11655-011-0757-4 DOI：

Zhi-kui Chen, Li-wu Lin, Jing Cai, et al. Effect of interstitial chemotherapy with ricin temperature-responsive gel for anti-breast cancer and immune regulation in rats. [J]. Chinese Journal of Integrative Medicine 19(1):48-53(2013) DOI： 10.1007/s11655-011-0757-4.

摘要

To explore the effect of ricin temperature response gel on breast cancer and its regulatory effect on immune function in rats. Ricin was purified by chromatography and identified by immunoblotting. The rat subcutaneously transplanted breast cancer model was established. Forty model rats with a tumor diameter of about 3.0 cm were subjected to the study. They were randomized into four groups equally: the model group and three treated groups (blank gel

ricin

ricin-gel) were administered with blank gel

ricin

and ricin temperature response gel via percutaneous intratumor injection

respectively. The tumor was isolated 10 days later for the estimation of tumor inhibition rate (TIR) by weighing

pathologic examination

and detection of tumor apoptosis-associated genes bcl-2 and bax with semiquantitative RT-PCR. Also

peripheral blood was obtained to test T-lymphocyte subsets

the killing function of lymphocytes

and the contents of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2). The outcomes were compared between groups. The TIR in the ricin-gel group was 61.8%

with the pathologic examination showing extensive tumor tissue necrosis. Compared with the model group

after ricin temperature response gel treatment

bcl-2 expression was down-regulated

bax expression was up-regulated

CD4+ lymphocytes and CD4+/CD8 /CD8+ ratio in peripheral blood were increased

the killing function of lymphocytes was enhanced

and the contents of TNF-α and IL-2 were elevated (P<0.05 or P<0.01). Intratumor injection of ricin temperature-responsive gel showed significant antitumor effect on breast cancer and could enhance the immune function in the tumor-bearing rat.

Abstract

ricin

ricin-gel) were administered with blank gel

ricin

and ricin temperature response gel via percutaneous intratumor injection

respectively. The tumor was isolated 10 days later for the estimation of tumor inhibition rate (TIR) by weighing

pathologic examination

and detection of tumor apoptosis-associated genes bcl-2 and bax with semiquantitative RT-PCR. Also

peripheral blood was obtained to test T-lymphocyte subsets

the killing function of lymphocytes

and the contents of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2). The outcomes were compared between groups. The TIR in the ricin-gel group was 61.8%

with the pathologic examination showing extensive tumor tissue necrosis. Compared with the model group

after ricin temperature response gel treatment

bcl-2 expression was down-regulated

bax expression was up-regulated

CD4+ lymphocytes and CD4+/CD8 /CD8+ ratio in peripheral blood were increased

the killing function of lymphocytes was enhanced

关键词

ricingelBreast Cancermesenchymal chemotherapyimmune regulation

Keywords

ricingelBreast Cancermesenchymal chemotherapyimmune regulation

references

Mc Pheson K, Steel CM, Dixon JM. ABC of breast disease. Breast cancer epidemiology risk factors, and genetics. BMJ 2000;321:624–628.

Ahmad N, Arif K, Faisal SM, Neyaz MK, Tayyab S, Owaris M. PLGA-microsphere mediated clearance of bilirubin in temporarily hyperbilirubinemic rats: an alternate strategy for the treatment of experimental jaundice. Biochim Biophys Acta 2006;1760:227–232.

Ravivarapu HB, Burton K, DeLuca PP. Polymer and microsphere blending to alter the release of a peptide from PLGA microspheres. Eur J Pharm Biopharm 2000;50:263–270.

Jain AK, Chalasani KB, Khar RK, Ahmed FJ, Diwan PV. Muco-adhesive multivesicular liposomes as an effective carrier for transmucosal insulin delivery. J Drug Target 2007;15:417–427.

Ghahremankhani AA, Dorkoosh F, Dinarvand R. PLGAPEG-PLGA tri-block copolymers as in situ gel-forming peptide delivery system: effect of formulation properties on peptide release. Pharm Dev Technol 2008;13:49–55.

Ruel-Gariepy E, Shive M, Bichara, Berrada M, Le Garrec D, Chenite A, et al. A thermosensitive chitosan-based hydrogel for the local delivery of paclitaxel. Eur J Pharm Biopharm 2004;57:53–63.

Mok TS, Kanekal S, Lin XR, Leung TW, Chan AT, Yeo W, et al. Pharmacokinetic study of intralesional cisplatin for the treatment of hepatocellular carcinoma. Cancer 2001;91:2369–2377.

Sandvig S, Grimmer S, Lversen TG, Rodal K, Torgersen ML, Nicoziani P, et al. Ricin transport into cells: studies of endocytosis and intracellular transport. Int J Med Microbiol 2000;290:415–420.

Sandvig K, van Deurs B. Entry of ricin and Shiga toxin into cells: molecular mechanisms and medical perspectives. EMBO J 2000;19:5943–5950.

Chen ZK, Lin LW, Yang YH, Cai HJ, Yang JJ, Cai MX. US-guided interstitial chemotherapy using paclitaxel temperature-responsive gel for the treatment of breast cancer. J Cancer Res Clin Oncol 2010;136:537–545.

Jeong B, Bae YH, Kim SW. Drug release f rom biodegradable injectable thermosensitive hydrogel of PEG-PLGA-PEG triblock copolymers. J Controll Release 2000;63:155–163.

Duvvuri S, Janoria KG, Pal D, Mitra AK. Controlled delivery of ganciclovir to the retina with drug-loaded Poly (d,Llactide-co-glycolide)(PLGA) microspheres dispersed in PLGA-PEG-PLGA gel: a novel intravitreal delivery system for the treatment of cytomegalovirus retinitis. J Ocul Pharmacol Ther 2007;23:264–274.

Rao PV, Jayaraj R, Bhaskar AS, Kumar O, Bhattacharya R, Saxena P, et al. Mechanism of ricin-induced apoptosis in human cervical cancer cells. Biochem Pharmacol 2005;69:855–865.

Gan YH, Peng SQ, Liu HY. Molecular mechanism of apoptosis induced by ricin in HeLa cells. Acta Pharmacol Sin 2000;21:243–248.

Licastro F, Morini MC, Bolognesi A, Stirpe F. Ricin induces the production of tumor necrosis factor-alpha and interleukin-1 beta by human peripheral-blood mononuclear cells. Biochem J 1993;294:517–520.

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