Experimental study on the molecular mechanism of anthraquinone cathartics in inducing melanosis coli

Jian-yong Chen; Feng Pan; Tao Zhang; Jin Xia; Yan-juan Li

doi:10.1007/s11655-011-0786-z

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Experimental study on the molecular mechanism of anthraquinone cathartics in inducing melanosis coli

Updated：2021-08-27

- Experimental study on the molecular mechanism of anthraquinone cathartics in inducing melanosis coli
- Chinese Journal of Integrative Medicine Vol. 17, Issue 7, Pages: 525-530(2011)
- Affiliations：
  
  1. Department of Digestive Diseases, Zhejiang Integrated Medical Hospital,Hangzhou,China
  2. Institute of Pi-Wei, Guangzhou Traditional Chinese Medical University,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by Zhejiang Provincial Funds of Natural Sciences (No. X206959) and the Key Project Item of Hangzhou Municipal Administration of Science and Technology (No. 2006533Q15)
- DOI：10.1007/s11655-011-0786-z
  CLC：
- Published：2011，
  
  Published Online：3 July 2011，
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Chen, Jy., Pan, F., Zhang, T. et al. Experimental study on the molecular mechanism of anthraquinone cathartics in inducing melanosis coli., Chin. J. Integr. Med. 17, 525–530 (2011). https://doi.org/10.1007/s11655-011-0786-z

Jian-yong Chen, Feng Pan, Tao Zhang, et al. Experimental study on the molecular mechanism of anthraquinone cathartics in inducing melanosis coli. [J]. Chinese Journal of Integrative Medicine 17(7):525-530(2011)
Chen, Jy., Pan, F., Zhang, T. et al. Experimental study on the molecular mechanism of anthraquinone cathartics in inducing melanosis coli., Chin. J. Integr. Med. 17, 525–530 (2011). https://doi.org/10.1007/s11655-011-0786-z DOI：

Jian-yong Chen, Feng Pan, Tao Zhang, et al. Experimental study on the molecular mechanism of anthraquinone cathartics in inducing melanosis coli. [J]. Chinese Journal of Integrative Medicine 17(7):525-530(2011) DOI： 10.1007/s11655-011-0786-z.

摘要

To explore the significance of colonic epithelial cell apoptosis and tumor necrosis factor α (TNF-α) changing in pathogenesis of melanosis coli (MC) in guinea pig and the molecular mechanism of rhubarb (Rhu) in inducing the disease

by means of using different dosages of Rhu to induce the disease. One hundred and forty-four male guinea pigs

clean grade

were randomized according to their body weight into 5 groups

the untreated normal group and the 4 Rhu groups treated

respectively

with different doses of Rhu

3 g/kg·d for low dose (Rhu-l) group

6 g/kg·d for moderate dose (Rhu-m) group

12 g/kg·d for high dose (Rhu-h) group and 24 g/kg·d for super-high dose (Rhu-s) group via gastric infusion. All animals were sacrificed 60 days later

their viscera were taken for observing the pathologic and morphologic changes with HE

melanin and melatonin staining

and the apoptosis of colonic epithelial cells was detected with TUNEL stain and transmission electric microscopy. In addition

the levels of TNF-α in serum and colonic tissue were measured using ELISA and RT-PCR. The pathological changes of MC could be found by naked eye in all Rhu groups

especially apparent at caecum and proximal end of colon

but did not found in gallbladder

jejunum and ileum. In normal guinea pigs

the colonic membrane was pink in color with no apparent pigment deposition. Membranous color deepened in the Rhu groups depending on the dosage of Rhu used. MC scoring showed the highest scores revealed in the Rhu-s group (6.00±0.00)

which was significantly different to those in the Rhu-l (3.86±0.69)

Rhu-m (4.43±0.79) and Rhu-h groups (4.88±0.35

all P<0.05). Levels of cell apoptosis in colon and TNF-α in serum in all Rhu groups were higher than those in the normal group (P<0.01)

but showed no significant difference among the Rhu groups (P>0.05). Moreover

a positive correlation was found in the degree of induced MC with apoptosis rate and TNF-α level. Rhu (anthraquinone purgatives) had apparent effect on inducing MC; its molecular mechanism is maybe to destroy intestinal mucosal barrier and advance proinflammatory factor TNF-α releasing

which leads to colonic epithelial cells apoptosis

and finally induce the change of MC due to the deposition of brown pigments

i.e. the macrophage phagocytized apoptotic body

on the colonic membrane.

Abstract

by means of using different dosages of Rhu to induce the disease. One hundred and forty-four male guinea pigs

clean grade

were randomized according to their body weight into 5 groups

the untreated normal group and the 4 Rhu groups treated

respectively

with different doses of Rhu

3 g/kg·d for low dose (Rhu-l) group

6 g/kg·d for moderate dose (Rhu-m) group

12 g/kg·d for high dose (Rhu-h) group and 24 g/kg·d for super-high dose (Rhu-s) group via gastric infusion. All animals were sacrificed 60 days later

their viscera were taken for observing the pathologic and morphologic changes with HE

melanin and melatonin staining

and the apoptosis of colonic epithelial cells was detected with TUNEL stain and transmission electric microscopy. In addition

the levels of TNF-α in serum and colonic tissue were measured using ELISA and RT-PCR. The pathological changes of MC could be found by naked eye in all Rhu groups

especially apparent at caecum and proximal end of colon

but did not found in gallbladder

jejunum and ileum. In normal guinea pigs

which was significantly different to those in the Rhu-l (3.86±0.69)

Rhu-m (4.43±0.79) and Rhu-h groups (4.88±0.35

all P<0.05). Levels of cell apoptosis in colon and TNF-α in serum in all Rhu groups were higher than those in the normal group (P<0.01)

but showed no significant difference among the Rhu groups (P>0.05). Moreover

which leads to colonic epithelial cells apoptosis

and finally induce the change of MC due to the deposition of brown pigments

i.e. the macrophage phagocytized apoptotic body

on the colonic membrane.

关键词

anthraquinoneextract of rhubarbguinea pigmelanosis colicolonic epithelial cell apoptosistumor necrosis factor-α

Keywords

anthraquinoneextract of rhubarbguinea pigmelanosis colicolonic epithelial cell apoptosistumor necrosis factor-α

references

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Willems M, van Buuren HR, de Krijger R. Anthranoid selfmedication causing rapid development of melanosis coli. Neth J Med 2003;61:22–24.

Freeman HJ. “Melanosis” in the small and large intestine. World J Gastroenterol 2008;14:4296–4299.

Russell NJ, Royland JE, McCawley EL. Danthron induced melanosis coli in the guinea pig. Proc West Pharmacol Soc 1980;23:277–280.

Xu SY, Bian RL, Chen X, eds. Experimental methodology of pharmacy. Beijing: People’s Medical Publishing House; 2002:1860–1866.

Russell NJ, Royland JE, McCauley EL. Melanosis coli in the guinea pig: a light and electron microscopic study. Proc West Pharmacol Soc 1982;25:193–195.

O’Connor KA, Johnson JD, Hansen MK, Ozdemirli M. Peripheral and central proinflammatory cytokine response to a severe acute stressor. Brain Res 2003;991:123–132.

Wilson JA. Tumor necrosis factor alpha and colitis-associated colon cancer. N Engl J Med 2008;358:2733–2734.

Ewing CA, Kalan M, Chucker F, Ozdemirli M. Melanosis coli involving pericolonic lymph nodes associated with the herbal laxative Swiss Kriss: a rare and incidental finding in a patient with colonic adenocarcinoma. Arch Pathol Lab Med 2004;128:565–567.

Wilson JA. Tumor necrosis factor alpha and colitis-associated colon cancer. N Engl J Med 2008;358:2733–2734.

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Beijing Institute of Traditional Chinese Medicine

Beijing Traditional Chinese Medicine Hospital Affiliated to Capital Medical University

Department of Hematology, the First Affi liated Hospital, Zhejiang University of Traditional Chinese Medicine

Department of Hepatology, Zhejiang Hospital of Traditional Chinese Medicine

Department of Traditional Chinese Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University

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