Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma

Peng-cheng Gu; Xin-sheng Fan; Chen-xue Jiang; Hui-qin Xu; Jing-hua Yu; Yu-ping Tang

doi:10.1007/s11655-011-0788-x

Your Location：

Home >

Browse articles >

Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma

Updated：2021-08-27

- Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma
- Chinese Journal of Integrative Medicine Vol. 17, Issue 7, Pages: 537-541(2011)
- Affiliations：
  
  1. Department of Pharmacology, Nanjing University of Chinese Medicine,Nanjing,China
  2. Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Chinese Medicine,Nanjing,China
  3. Clinical Trials Center, Nanjing University of Chinese Medicine,Nanjing,China
- Author bio：
- Funds：
  
  Supported by a Grant-in-aid for Scientific Research in the 11th Five-Year Plan, the Ministry of Science and Technology of P. R. China (No. 2006BAI06A02-01) and Natural Science Foundation of China (No. 81072748)
- DOI：10.1007/s11655-011-0788-x
  CLC：
- Published：2011，
  
  Published Online：3 July 2011，
Scan for full text
Gu, Pc., Fan, Xs., Jiang, Cx. et al. Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma., Chin. J. Integr. Med. 17, 537–541 (2011). https://doi.org/10.1007/s11655-011-0788-x

Peng-cheng Gu, Xin-sheng Fan, Chen-xue Jiang, et al. Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma. [J]. Chinese Journal of Integrative Medicine 17(7):537-541(2011)
Gu, Pc., Fan, Xs., Jiang, Cx. et al. Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma., Chin. J. Integr. Med. 17, 537–541 (2011). https://doi.org/10.1007/s11655-011-0788-x DOI：

Peng-cheng Gu, Xin-sheng Fan, Chen-xue Jiang, et al. Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma. [J]. Chinese Journal of Integrative Medicine 17(7):537-541(2011) DOI： 10.1007/s11655-011-0788-x.

摘要

San’ao Decoction (三拗汤

SAD)

as a representative Chinese medicine (CM) formula

was chosen to evaluate the effect of airway inflammation and hyperresponsiveness on the lipopolysaccharide (LPS) enhanced asthma model. The asthma model was reproduced in the Balb/C mice sensitized by ovalbumin (OVA)

challenged by OVA and LPS. After Balb/C mice’s administration of a dose (0.0024 g/kg) of dexamethasone acetate

and three doses (2.2 g/kg

4.4 g/kg and 8.8 g/kg) of SAD

airway inflammation and responsiveness were observed. The airway inflammation was detected by counting bronchoalveolar lavage fluid (BALF) cells and lung histopathology. Also

differential expressions of interferon-r (IFN-γ)

interleukin-4 (IL-4)

and IL-5 in the supernatants of BALF were examined. The changes in airway responsiveness indicated by lung resistance (RL) and stimulated by acetylcholine (Ach) were determined. Small-dose SAD hardly inhibit airway inflammation or hyperresponsiveness in the LPS-enhanced asthma

while medium-dose and high-dose SAD significantly inhibited the airway hyperresponsiveness

and to some extent

reduced airway inflammation. Meanwhile

the small-dose

medium-dose

and high-dose SAD promoted Th1-type cytokines (IFN-γ) and reduced Th2-type cytokines (IL-4

IL-5) to different extents

which led to a Th1/Th2 balance. SAD has a good therapeutic effect on airway hyperresponsiveness in the LPS-enhanced asthma model

but its definite influence on airway inflammation is not remarkable.

Abstract

San’ao Decoction (三拗汤

SAD)

as a representative Chinese medicine (CM) formula

challenged by OVA and LPS. After Balb/C mice’s administration of a dose (0.0024 g/kg) of dexamethasone acetate

and three doses (2.2 g/kg

4.4 g/kg and 8.8 g/kg) of SAD

airway inflammation and responsiveness were observed. The airway inflammation was detected by counting bronchoalveolar lavage fluid (BALF) cells and lung histopathology. Also

differential expressions of interferon-r (IFN-γ)

interleukin-4 (IL-4)

while medium-dose and high-dose SAD significantly inhibited the airway hyperresponsiveness

and to some extent

reduced airway inflammation. Meanwhile

the small-dose

medium-dose

and high-dose SAD promoted Th1-type cytokines (IFN-γ) and reduced Th2-type cytokines (IL-4

IL-5) to different extents

which led to a Th1/Th2 balance. SAD has a good therapeutic effect on airway hyperresponsiveness in the LPS-enhanced asthma model

but its definite influence on airway inflammation is not remarkable.

关键词

San’ao DecoctionlipopolysaccharideAsthma

Keywords

San’ao DecoctionlipopolysaccharideAsthma

references

Gu PC, Xu HQ, Fan XS, Liu CD. Reproduction of asthma model in ovalbumin-sensitized mice by ovalbumin challenge and lipopolysaccharide induction. Chin J Tuberc Respir Dis (Chin) 2010;33(1):56–59.

Jiang XB, Zhu Y, Yin KS. Reproduction of severe asthma model in ovalbumin-sensitized mice by ovalbumin challenge and respiratory syncytial virus induction. Chin J Tuberc Respir Dis (Chin) 2006;29:344–345.

Tong HJ, Fan XS, Xu HQ, Wang L, Cui J. Reproduction of a type of viral asthma model in mice and its evaluation. J Xi’an Jiaotong Univ (Chin) 2008;29:349–352.

Zhang Y, Tong HJ, Yu JH, Gu PC, Fan XS, Xu HQ. Effects of San’ao Decoction and its analogous prescriptions on airway inflammation in mice with respiratory syncytial virus- and ovalbumin-induced asthma. J Chin Integr Med (Chin) 2009;7:354–359.

Huang WC, Kuo ML, Li ML, Yang RC, Liou CJ, Shen JJ. Gynostemma pentaphyllum decreases allergic reactions in a murine asthmatic model. Am J Chin Med 2008;36:579–592.

Murakami D, Yamada H, Yajima T, Masuda A, Komune S, Yoshikai Y. Lipopolysaccharide inhalation exacerbates allergic airway inflammation by activating mast cells and promoting Th2 responses. Clin Exp Allergy 2007;37:339–347.

Gerhold K, Blumchen K, Bock A, Seib C, Stock P, Kallinich T, et al. Endotoxins prevent murine IgE production, T(H)2 immune responses, and development of airway eosinophilia but not airway hyperreactivity. J Allergy Clin Immunol 2002;110:110–116.

Liu AH. Endotoxin exposure in allergy and asthma: reconciling a paradox. J Allergy Clin Immunol 2002;109:379–392.

Delayre-Orthez C, de Blay F, Frossard N, Pons F. Dose-dependent effects of endotoxins on allergen sensitization and challenge in the mouse. Clin Exp Allergy 2004;34:1789–1795.

Tulic MK, Holt PG, Sly PD. Modification of acute and late-phase allergic responses to ovalbumin with lipopolysaccharide. Int Arch Allergy Immunol 2002;129:119–128.

Ji LL, Xu Li, Fan XS, Yu JH, Tang YP. Effects of San’ao Decoction and its analogous prescriptions on the latent period of inducing asthma and eosinophilia in a guinea pig model of OVA-induced asthma. J Nanjing Univ Tradit Chin Med (Chin) 2008;24(6):391–393.

Xu HQ, Gu PC, Fan XS, Tong HJ, Zhang Y, Chen JD. Effect of San’ao Decoction and its analogous prescription on airway responsiveness in asthmatic mice induced by ovalbumin sensitisation and RSV infection. Modern Tradit Chin Med Mater Med (Chin) 2009;11:702–706

Hou JY. Pharmacology of Chinese materia medica. Beijing: China Press of Traditional Chinese Medicine; 2002:27–30.

Xing GX, Li N, Yang MY, Cui LJ, Wang T. Research progress on natural amygdalin. Chin Tradit Patent Med (Chin) 2003;25:1007–1009.

FULL TEXT LINK

More>

Views

847

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats

Tripterygium polyglycosid attenuates the established airway inflammation in asthmatic mice

Effect of safflor yellow injection on inhibiting lipopolysaccharide-induced pulmonary inflammatory injury in mice

Effects and mechanisms of actions of Chinese herbal medicines for asthma

Salvianolic acid B inhibits the TLR4-NFκB-TNFα pathway and attenuates neonatal rat cardiomyocyte injury induced by lipopolysaccharide

Related Author

No data

Related Institution

State Key Laboratory of New-Tech for Chinese Medicine Pharmaceutical Process

Pharmacy College, Jinan University

Department of Respiratory Medicine, the First People’s Hospital of Hangzhou

Department of Laboratory, the Second Affiliated Hospital of Zhejiang University Medical College

Department of Allergy, the Second Affiliated Hospital of Zhejiang University Medical College

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰