Proliferation-inhibiting and apoptosis-inducing effects of ursolic acid and oleanolic acid on multi-drug resistance cancer cells in vitro

Jian-zhen Shan; Yan-yan Xuan; Shu-qin Ruan; Mei Sun

doi:10.1007/s11655-011-0815-y

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Proliferation-inhibiting and apoptosis-inducing effects of ursolic acid and oleanolic acid on multi-drug resistance cancer cells in vitro

Updated：2021-08-27

- Proliferation-inhibiting and apoptosis-inducing effects of ursolic acid and oleanolic acid on multi-drug resistance cancer cells in vitro
- Chinese Journal of Integrative Medicine Vol. 17, Issue 8, Pages: 607-611(2011)
- Affiliations：
  
  1. Department of Oncology, the Second Affiliated Hospital, School of Medicine, Zhejiang University,Hangzhou,China
  2. Cancer Institute, School of Medicine, Zhejiang University,Hangzhou,China
  3. Department of Traditional Chinese Medicine, the Second Affiliated Hospital, School of Medicine, Zhejiang University,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Plan for Supporting High Technique Research and Development (863 Plan, No. 2006AA02Z341), and the Supporting Items of Zhejiang Ministry of Sciences and Technology (No. 2008C30037)
- DOI：10.1007/s11655-011-0815-y
  CLC：
- Published：2011，
  
  Published Online：9 August 2011，
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Shan, Jz., Xuan, Yy., Ruan, Sq. et al. Proliferation-inhibiting and apoptosis-inducing effects of ursolic acid and oleanolic acid on multi-drug resistance cancer cells in vitro, ., Chin. J. Integr. Med. 17, 607 (2011). https://doi.org/10.1007/s11655-011-0815-y

Jian-zhen Shan, Yan-yan Xuan, Shu-qin Ruan, et al. Proliferation-inhibiting and apoptosis-inducing effects of ursolic acid and oleanolic acid on multi-drug resistance cancer cells in vitro. [J]. Chinese Journal of Integrative Medicine 17(8):607-611(2011)
Shan, Jz., Xuan, Yy., Ruan, Sq. et al. Proliferation-inhibiting and apoptosis-inducing effects of ursolic acid and oleanolic acid on multi-drug resistance cancer cells in vitro, ., Chin. J. Integr. Med. 17, 607 (2011). https://doi.org/10.1007/s11655-011-0815-y DOI：

Jian-zhen Shan, Yan-yan Xuan, Shu-qin Ruan, et al. Proliferation-inhibiting and apoptosis-inducing effects of ursolic acid and oleanolic acid on multi-drug resistance cancer cells in vitro. [J]. Chinese Journal of Integrative Medicine 17(8):607-611(2011) DOI： 10.1007/s11655-011-0815-y.

摘要

To investigate the proliferation-inhibiting and apoptosis-inducing effects of ursolic acid (UA) and oleanolic acid (OA) on multi-drug resistance (MDR) cancer cells in vitro. UA and OA in different concentrations (0–100 μmol/L) were added separately to cultures of different cancer cell lines

including the human colon cancer cell lines SW480 and SW620

human acute myelocytic leukemia cancer cell lines HL60 and HL60/ADR

human chronic myelogenous leukemia cell lines K562 and K562/ADR

and the human breast cancer cell lines MCF-7 and MCF-7/ADR. Effects of UA and OA on cell proliferation were detected by 3-(4

5-dimethyl-2-thiazole)-2-5-biphenly-tetrazole bromide (MTT) method and effects on cell apoptosis were tested by flow cytometry (FCM) and Western blot at 24

and 72 h after treatment. Both UA and OA showed significant inhibition on parent and MDR cell lines in a time- and concentration-dependent manner; the drug-resistant multiple of them on K562 and K562/ADR as well as on HL60 and HL60/ADR was 1; the effects of UA were better than those of OA in inhibiting cell growth of solid colonic cancer and breast cancer. After SW480 cells were treated by UA at the concentrations of 0–40 μmol/L for 48 h

FCM showed that annexin V (AV) positive cells and hypodiploid peak ratio increased along with the increase in the drug’s concentrations; and Western blot found that expressions of Bcl-2

Bcl-xL and survivin decreased in a concentration-dependent manner. Both UA and OA have antitumor effects on cancer cells with MDR

and the optimal effect is shown by UA on colonic cancer cells. Also

UA shows cell apoptosis-inducing effect on SW480

possibly by way of down-regulating the expressions of apoptosis antagonistic proteins

Bcl-2

Bcl-xL

and survivin.

Abstract

including the human colon cancer cell lines SW480 and SW620

human acute myelocytic leukemia cancer cell lines HL60 and HL60/ADR

human chronic myelogenous leukemia cell lines K562 and K562/ADR

and the human breast cancer cell lines MCF-7 and MCF-7/ADR. Effects of UA and OA on cell proliferation were detected by 3-(4

5-dimethyl-2-thiazole)-2-5-biphenly-tetrazole bromide (MTT) method and effects on cell apoptosis were tested by flow cytometry (FCM) and Western blot at 24

FCM showed that annexin V (AV) positive cells and hypodiploid peak ratio increased along with the increase in the drug’s concentrations; and Western blot found that expressions of Bcl-2

Bcl-xL and survivin decreased in a concentration-dependent manner. Both UA and OA have antitumor effects on cancer cells with MDR

and the optimal effect is shown by UA on colonic cancer cells. Also

UA shows cell apoptosis-inducing effect on SW480

possibly by way of down-regulating the expressions of apoptosis antagonistic proteins

Bcl-2

Bcl-xL

and survivin.

关键词

Ursolic AcidOleanolic Acidmulti-drug resistance cancer cellproliferation inhibitionapoptosis

Keywords

Ursolic AcidOleanolic Acidmulti-drug resistance cancer cellproliferation inhibitionapoptosis

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