Antifibrotic effect of the Chinese herbs Modified Danggui Buxue Decoction (加味当归补血汤) on adriamycin-induced nephropathy in rats

Ming-gang Wei; Wei Sun; Pei-hua Xiong; Jia-de Shao

doi:10.1007/s11655-011-0816-x

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Antifibrotic effect of the Chinese herbs Modified Danggui Buxue Decoction (加味当归补血汤) on adriamycin-induced nephropathy in rats

Updated：2021-08-27

- Antifibrotic effect of the Chinese herbs Modified Danggui Buxue Decoction (加味当归补血汤) on adriamycin-induced nephropathy in rats
- Chinese Journal of Integrative Medicine Vol. 18, Issue 8, Pages: 591-598(2012)
- Affiliations：
  
  1. The First Affiliated Hospital of Soochow University,Jiangsu Province,Suzhou,China
  2. The Hospital of Traditional Chinese Medicine of Jiangsu Province,Nanjing,China
- Author bio：
- Funds：
  
  Supported by the Grant from Soochow University Natural Science Foundation of Young Teacher (No. Q3122937) and the Natural Science Foundation of Bureau of Science and Technology of Suzhou (No. SYS201016)
- DOI：10.1007/s11655-011-0816-x
  CLC：
- Published：2012，
  
  Published Online：30 July 2011，
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Wei, Mg., Sun, W., Xiong, Ph. et al. Antifibrotic effect of the Chinese herbs Modified Danggui Buxue Decoction (加味当归补血汤) on adriamycin-induced nephropathy in rats., Chin. J. Integr. Med. 18, 591–598 (2012). https://doi.org/10.1007/s11655-011-0816-x

Ming-gang Wei, Wei Sun, Pei-hua Xiong, et al. Antifibrotic effect of the Chinese herbs Modified Danggui Buxue Decoction (加味当归补血汤) on adriamycin-induced nephropathy in rats. [J]. Chinese Journal of Integrative Medicine 18(8):591-598(2012)
Wei, Mg., Sun, W., Xiong, Ph. et al. Antifibrotic effect of the Chinese herbs Modified Danggui Buxue Decoction (加味当归补血汤) on adriamycin-induced nephropathy in rats., Chin. J. Integr. Med. 18, 591–598 (2012). https://doi.org/10.1007/s11655-011-0816-x DOI：

Ming-gang Wei, Wei Sun, Pei-hua Xiong, et al. Antifibrotic effect of the Chinese herbs Modified Danggui Buxue Decoction (加味当归补血汤) on adriamycin-induced nephropathy in rats. [J]. Chinese Journal of Integrative Medicine 18(8):591-598(2012) DOI： 10.1007/s11655-011-0816-x.

摘要

To investigate the antifibrotic effect of the Chinese herbs Modified Danggui Buxue Decoction (加味当归补血汤

MDBD) on adraimycin-induced nephropathy in rats. Thirty-two male Sprague Dawley albino rats were randomly divided into 4 groups: the control

model

and two treatment groups

with 8 in each group. Nephropathy was induced in the latter 3 groups by intravenous injection of adriamycin. Rats in the two treatment groups received intragastric administration of benazepri (a positive control) or MDBD

which is composed of extracts of Radix Angelicae sinensis

Astragalus membranaceus (Fisch.) Bge and Rhizoma chuanxiong. Serum albumin

blood lipids

24-h urine protein and urine N-acetyl-b-D-glucosaminidase (NAG) were measured every 2 weeks. The ratio of kidney to body weight was measured. The expressions of extracellular matrix proteins in the renal cortex

including colleagen IV (Col-IV) and fibronectin (FN)

were examined by immunohistochemistry

and the transcription of genes encoding transforming growth factor β1 (TGF-β1)

the tissue inhibitors of matrix metalloproteinase 1 (TIMP-1) and matrix metalloproteinase 9 (MMP-9) were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) at the end of the 8-week treatment. Compared with the untreated rats in the model group

MDBD significantly increased serum albumin

lowered the blood lipids and decreased the ratio of kidney to body weight. MDBD significantly reduced the excretion levels of urinary protein and NAG as well as the accumulation of extracellular matrix (ECM)

including Col-IV and FN

in the renal cortex. Further

MDBD decreased TIMP-1 and TGF-β1 gene expressions and increased MMP-9 gene expression in the kidney. MDBD was effective in treating the rat model of nephropathy. The clinical benefit was associated with reduction of renal fibrosis. The antifibrotic effect of MDBD may be mediated through the regulation of TIMP-1

MMP and TGF-β1 gene expressions.

Abstract

To investigate the antifibrotic effect of the Chinese herbs Modified Danggui Buxue Decoction (加味当归补血汤

MDBD) on adraimycin-induced nephropathy in rats. Thirty-two male Sprague Dawley albino rats were randomly divided into 4 groups: the control

model

and two treatment groups

which is composed of extracts of Radix Angelicae sinensis

Astragalus membranaceus (Fisch.) Bge and Rhizoma chuanxiong. Serum albumin

blood lipids

including colleagen IV (Col-IV) and fibronectin (FN)

were examined by immunohistochemistry

and the transcription of genes encoding transforming growth factor β1 (TGF-β1)

MDBD significantly increased serum albumin

including Col-IV and FN

in the renal cortex. Further

MMP and TGF-β1 gene expressions.

关键词

Chinese herbsadriamycin-induced nephropathy ratmechanismantifibrosis effect

Keywords

Chinese herbsadriamycin-induced nephropathy ratmechanismantifibrosis effect

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