Celastrus Orbiculatus Extract inhibits tumor angiogenesis by targeting vascular endothelial growth factor signaling pathway and shows potent antitumor activity in hepatocarcinomas in Vitro and in Vivo

Ya-yun Qian; Hua Zhang; Ying Hou; Lin Yuan; Guo-qing Li; Shi-yu Guo; Tadashi Hisamits; Yan-qing Liu

doi:10.1007/s11655-011-0819-7

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Celastrus Orbiculatus Extract inhibits tumor angiogenesis by targeting vascular endothelial growth factor signaling pathway and shows potent antitumor activity in hepatocarcinomas in Vitro and in Vivo

Updated：2021-08-27

- Celastrus Orbiculatus Extract inhibits tumor angiogenesis by targeting vascular endothelial growth factor signaling pathway and shows potent antitumor activity in hepatocarcinomas in Vitro and in Vivo
- Chinese Journal of Integrative Medicine Vol. 18, Issue 10, Pages: 752-760(2012)
- Affiliations：
  
  1. Institute of Traditional Chinese Medicine and Western Medicine, School of Medicine, Yangzhou University,Jiangsu Province,Yangzhou,China
  2. Department of Image, 97th Hospital of People’s Liberation Army,Jiangsu Province,Xuzhou,China
  3. Department of Physiology, School of Medicine, Showa University,Tokyo,Japan
- Author bio：
- Funds：
  
  Supported by Plans of Colleges and Universities in Jiangsu Province to Postgraduate Research and Innovation (No. CX09B-321Z) and State Administration of Traditional Chinese Medicine of People’s Republic of China (No. 04-05ZP35)
- DOI：10.1007/s11655-011-0819-7
  CLC：
- Published：2012，
  
  Published Online：30 July 2011，
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Qian, Yy., Zhang, H., Hou, Y. et al. , Celastrus Orbiculatus Extract inhibits tumor angiogenesis by targeting vascular endothelial growth factor signaling pathway and shows potent antitumor activity in hepatocarcinomas in Vitro and in Vivo, ., Chin. J. Integr. Med. 18, 752–760 (2012). https://doi.org/10.1007/s11655-011-0819-7

Ya-yun Qian, Hua Zhang, Ying Hou, et al. Celastrus Orbiculatus Extract inhibits tumor angiogenesis by targeting vascular endothelial growth factor signaling pathway and shows potent antitumor activity in hepatocarcinomas in Vitro and in Vivo. [J]. Chinese Journal of Integrative Medicine 18(10):752-760(2012)
Qian, Yy., Zhang, H., Hou, Y. et al. , Celastrus Orbiculatus Extract inhibits tumor angiogenesis by targeting vascular endothelial growth factor signaling pathway and shows potent antitumor activity in hepatocarcinomas in Vitro and in Vivo, ., Chin. J. Integr. Med. 18, 752–760 (2012). https://doi.org/10.1007/s11655-011-0819-7 DOI：

Ya-yun Qian, Hua Zhang, Ying Hou, et al. Celastrus Orbiculatus Extract inhibits tumor angiogenesis by targeting vascular endothelial growth factor signaling pathway and shows potent antitumor activity in hepatocarcinomas in Vitro and in Vivo. [J]. Chinese Journal of Integrative Medicine 18(10):752-760(2012) DOI： 10.1007/s11655-011-0819-7.

摘要

Celastrus orbiculatus Thunb. has been used for thousands of years in China as a remedy against cancer and inflammatory diseases. This study aims to investigate whether C. orbiculatus extract (COE) could inhibit angiogenesis

which is the pivotal step in tumor growth

invasiveness

and metastasis. In this study

the extract from the stem of C. orbiculatus was used. Mouse hepatic carcinoma cells (Hepa1-6) were treated with COE in different nontoxic concentrations (10

and 160 μg/mL). The mRNA and protein expression levels of vascular endothelial growth factor (VEGF) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot

respectively; the active fractions were further tested on C57BL/6 mice and human umbilical vein endothelial cells (HUVEC) for any antiangiogenic effects. COE significantly inhibited proliferation and induced apoptosis in Hepa1-6 cells and inhibited VEGF expression at both mRNA and protein levels. Furthermore

this agent inhibited the formation of the capillary-like structure in primary cultured HUVEC in a dose-dependent manner. In vivo

COE significantly reduced the volume and weight of solid tumors with low adverse effects and decreased tumor angiogenesis. In summary

COE could be used to treat hepatic carcinoma. The mechanisms of the antitumor activity of COE may be due to its effects against tumor angiogenesis by targeting the VEGF protein.

Abstract

which is the pivotal step in tumor growth

invasiveness

and metastasis. In this study

the extract from the stem of C. orbiculatus was used. Mouse hepatic carcinoma cells (Hepa1-6) were treated with COE in different nontoxic concentrations (10

and 160 μg/mL). The mRNA and protein expression levels of vascular endothelial growth factor (VEGF) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot

this agent inhibited the formation of the capillary-like structure in primary cultured HUVEC in a dose-dependent manner. In vivo

COE significantly reduced the volume and weight of solid tumors with low adverse effects and decreased tumor angiogenesis. In summary

COE could be used to treat hepatic carcinoma. The mechanisms of the antitumor activity of COE may be due to its effects against tumor angiogenesis by targeting the VEGF protein.

关键词

antitumorCelastrus orbiculatus Thunb.angiogenesisvascular endothelial growth factor

Keywords

antitumorCelastrus orbiculatus Thunb.angiogenesisvascular endothelial growth factor

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Department of Cardiovascular, Guang’anmen Hospital, China Academy of Chinese Medical Sciences

Department of Shang Han Teaching and Research, Hubei University of Traditional Chinese Medicine

Department of Immunology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences

Department of Molecular Biology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences

Traditional Chinese Medicine Research and Development Center, Guang’anmen Hospital, China Academy of Chinese Medical Sciences

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