Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids

Xiao-yu Liu; Qi Wang; Shi-jin Xia; Jian-hua Huang; Zi-yin Shen; Hao Xu

doi:10.1007/s11655-011-0848-2

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Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids

Updated：2021-08-27

- Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids
- Chinese Journal of Integrative Medicine Vol. 17, Issue 9, Pages: 704-709(2011)
- Affiliations：
  
  1. Shanghai Traditional Chinese Medicine Clinical Center of Cardio-cerebrovascular Diseases in Tenth People’s Hospital of Tongji University,Shanghai,China
  2. Shanghai Biochip Co., Ltd.,Shanghai,China
  3. Huadong Hospital Affiliated to Fudan University,Shanghai,China
  4. Institute of Integrative Medicine of Fudan University,Shanghai,China
  5. National Integrative Medicine Center for Cardiovascular Diseases, China-Japan Friendship Hospital,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30500681, 30973845), Major State Basic Research Development Program of China (No. 2007CB507406), and Traditional Chinese Medicine Modernization Programs of Shanghai Science and Technology Committee (No. 09dZ1975000)
- DOI：10.1007/s11655-011-0848-2
  CLC：
- Published：2011，
  
  Published Online：11 September 2011，
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Liu, Xy., Wang, Q., Xia, Sj. et al. Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids., Chin. J. Integr. Med. 17, 704 (2011). https://doi.org/10.1007/s11655-011-0848-2

Xiao-yu Liu, Qi Wang, Shi-jin Xia, et al. Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids. [J]. Chinese Journal of Integrative Medicine 17(9):704-709(2011)
Liu, Xy., Wang, Q., Xia, Sj. et al. Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids., Chin. J. Integr. Med. 17, 704 (2011). https://doi.org/10.1007/s11655-011-0848-2 DOI：

Xiao-yu Liu, Qi Wang, Shi-jin Xia, et al. Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids. [J]. Chinese Journal of Integrative Medicine 17(9):704-709(2011) DOI： 10.1007/s11655-011-0848-2.

摘要

To study the characteristics of lymphocyte nuclear factor kappa B (NF-κB) signal transduction kinase-related molecular mRNA differential expressions at various month age segments in aging process and the intervening effect of Epimedium flavonoids (EF) on it. Sixty SD rats were divided into six groups

according to animals’ age

i.e.

the 3 days (d) group

the 4 months (m) group

the 10 m group

the 18 m group

the 27 m group

and the 27 m+EF group. RNA was extracted from separated splenic lymphocytes. Adopting NF-κB signal path functional genome oligonucleotide gene-chip (128 related genes)

the integral characteristics and differences of NF-κB signal transduction kinase-related mRNA expressions were determined

and the intervening effect of EF was examined. The mean level of the NF-κB signal transduction kinase-related mRNA expressions in rats’ splenic lymphocytes lowered with aging; the highest expression was presented at 3 d after birth

and then

it lowered gradually

with the lowest level at 18 m or 27 m. After EF intervention

the expression level was raised to the 10–18 m level in the aged rats. The changing rules of lymphocyte NF-κB-signal-transduction-kinase-related mRNA expressions in various stages of aging are helpful for selecting the well time for preventing and intervening aging

and will also give a hint to the molecular index for assessment of senility retarding researches.

Abstract

according to animals’ age

i.e.

the 3 days (d) group

the 4 months (m) group

the 10 m group

the 18 m group

the 27 m group

and the 27 m+EF group. RNA was extracted from separated splenic lymphocytes. Adopting NF-κB signal path functional genome oligonucleotide gene-chip (128 related genes)

the integral characteristics and differences of NF-κB signal transduction kinase-related mRNA expressions were determined

and then

it lowered gradually

with the lowest level at 18 m or 27 m. After EF intervention

and will also give a hint to the molecular index for assessment of senility retarding researches.

关键词

nuclear factor-κB signal transduction kinasegene-chiplymphocyteagingimmunosenescenceEpimedium flavonoidsmRNA

Keywords

nuclear factor-κB signal transduction kinasegene-chiplymphocyteagingimmunosenescenceEpimedium flavonoidsmRNA

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Institute of Chinese Medicine, The Chinese University of Hong Kong

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Medical and Pharmacological Institute of Yangzhou University

Pharmacology Department, Beijing Key Laboratory for Brain Aging, Xuanwu Hospital of Capital University of Medical Science

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