Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells

Yue-rong Jiang; Yu Miao; Lin Yang; Mei Xue; Chun-yu Guo; Xiao-juan Ma; Hui-jun Yin; Da-zhuo Shi; Ke-ji Chen

doi:10.1007/s11655-011-0849-1

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Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells

Updated：2021-08-27

- Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells
- Chinese Journal of Integrative Medicine Vol. 18, Issue 1, Pages: 30-33(2012)
- Affiliations：
  
  Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Developing Plan for Basic Key Items (973 Plan, No. 2006CB504803)
- DOI：10.1007/s11655-011-0849-1
  CLC：
- Published：2012，
  
  Published Online：12 October 2011，
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Jiang, Yr., Miao, Y., Yang, L. et al. Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells., Chin. J. Integr. Med. 18, 30–33 (2012). https://doi.org/10.1007/s11655-011-0849-1

Yue-rong Jiang, Yu Miao, Lin Yang, et al. Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells. [J]. Chinese Journal of Integrative Medicine 18(1):30-33(2012)
Jiang, Yr., Miao, Y., Yang, L. et al. Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells., Chin. J. Integr. Med. 18, 30–33 (2012). https://doi.org/10.1007/s11655-011-0849-1 DOI：

Yue-rong Jiang, Yu Miao, Lin Yang, et al. Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors’ expression in endothelial cells. [J]. Chinese Journal of Integrative Medicine 18(1):30-33(2012) DOI： 10.1007/s11655-011-0849-1.

摘要

To investigate the effects of drug-containing serum of Chinese herbal compound

Xiongshao Capsule (芎芍胶囊

for activating-blood) and Huanglian Capsule (黄连胶囊

for dispellingtoxin) on the oxidized low-density lipoprotein (ox-LDL)-induced inflammatory factors in human umbilical vein endothelial cells (HUVECs). Thirty-two rats were randomly divided into four groups: the blank control group treated with distilled water

the positive control group treated with simvastatin (1.8 mg/kg)

the test group I treated with Chinese herbal compound of XS (0.135 g/kg)

and the test group II treated with Chinese herbal compound of XS (0.135 g/kg) and HL (0.135 g/kg). All the treatments were administered for 7 successive days by gastrogavage. Rats’ blood serum was harvested 1 h after the last administration to prepare respective drugcontaining serum. HUVECs were exposed to ox-LDL (100 μg/mL) to induce cell injury model and incubated with corresponding drug-containing serum for 24 h. Untreated HUVECs were set for blank control. Levels of interleukin-6 (IL-6)

tumor necrosis factor-α (TNF-α)

and soluble intercellular adhesion molecule-1 (sICAM-1) in supernatant of cultured HUVECs were determined by enzyme-linked immunosorbent assay (ELISA). HUVEC surface expressions of ICAM-1 and E-selectin were determined by flow cytometry. Levels of IL-6

TNF-α

and sICAM-1 in the supernatant of HUVECs as well as the cell surface expressions of ICAM-1 and E-selectin significantly increased after 24-h ox-LDL stimulation (P<0.01)

while the abnormal elevations

except sICAM-1 in the test group I

were all reduced in the treated groups (the positive control and the two test groups) significantly (P<0.01 or P<0.05). Besides

the effect in the test group II seemed somewhat higher than that in the test group I but with no statistical significance (P>0.05). Drug-containing serum of XS plus HL has a certain inhibitory effect on the vascular endothelial inflammation response induced by ox-LDL.

Abstract

To investigate the effects of drug-containing serum of Chinese herbal compound

Xiongshao Capsule (芎芍胶囊

for activating-blood) and Huanglian Capsule (黄连胶囊

the positive control group treated with simvastatin (1.8 mg/kg)

the test group I treated with Chinese herbal compound of XS (0.135 g/kg)

tumor necrosis factor-α (TNF-α)

TNF-α

and sICAM-1 in the supernatant of HUVECs as well as the cell surface expressions of ICAM-1 and E-selectin significantly increased after 24-h ox-LDL stimulation (P<0.01)

while the abnormal elevations

except sICAM-1 in the test group I

were all reduced in the treated groups (the positive control and the two test groups) significantly (P<0.01 or P<0.05). Besides

关键词

activating blood and dispelling toxindrug-containing serumendothelial celloxidized low-density lipoproteininflammation

Keywords

activating blood and dispelling toxindrug-containing serumendothelial celloxidized low-density lipoproteininflammation

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Related Institution

Xiyuan Hospital, China Academy of Chinese Medical Sciences

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Ministry of Education), Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College

Department of Pharmacology, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University

Key Laboratory of Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine

Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine

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