Ursolic acid inhibits T-cell activation through modulating nuclear factor-κ B signaling

Guang Zeng; Jiang Chen; Qing-hua Liang; Wan-hui You; Han-jun Wu; Xin-gui Xiong

doi:10.1007/s11655-011-0858-0

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Ursolic acid inhibits T-cell activation through modulating nuclear factor-κ B signaling

Updated：2021-08-27

- Ursolic acid inhibits T-cell activation through modulating nuclear factor-κ B signaling
- Chinese Journal of Integrative Medicine Vol. 18, Issue 1, Pages: 34-39(2012)
- Affiliations：
  
  1. Institute of Integrative Medicine, Xiangya Hospital, Central South University,Changsha,China
  2. Telemedicine Centre, Xiangya Hospital, Central South University,Changsha,China
- Author bio：
- Funds：
  
  Supported by the Natural Science Foundation of China (No. 30371833) and the Science and Technology Project Foundation of Hunan Science and Technology Bureau (No. 2007SK3030)
- DOI：10.1007/s11655-011-0858-0
  CLC：
- Published：2012，
  
  Published Online：12 October 2011，
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Zeng, G., Chen, J., Liang, Qh. et al. Ursolic acid inhibits T-cell activation through modulating nuclear factor-κ B signaling., Chin. J. Integr. Med. 18, 34–39 (2012). https://doi.org/10.1007/s11655-011-0858-0

Guang Zeng, Jiang Chen, Qing-hua Liang, et al. Ursolic acid inhibits T-cell activation through modulating nuclear factor-κ B signaling. [J]. Chinese Journal of Integrative Medicine 18(1):34-39(2012)
Zeng, G., Chen, J., Liang, Qh. et al. Ursolic acid inhibits T-cell activation through modulating nuclear factor-κ B signaling., Chin. J. Integr. Med. 18, 34–39 (2012). https://doi.org/10.1007/s11655-011-0858-0 DOI：

Guang Zeng, Jiang Chen, Qing-hua Liang, et al. Ursolic acid inhibits T-cell activation through modulating nuclear factor-κ B signaling. [J]. Chinese Journal of Integrative Medicine 18(1):34-39(2012) DOI： 10.1007/s11655-011-0858-0.

摘要

To investigate the effects of ursolic acid (UA) on T-cell proliferation and activation

as well as to examine its effect on nuclear factor-κB (NF-κB) signaling pathway in T cells. T-cells isolated from BALB/c mice were incubated with UA at concentrations ranging from 5–30 μmol/L in the presence of phorbol 12-myristate 13-acetate (PMA) or PMA plus ionomycin. The proliferation of T cells was measured by the MTT assay. The expressions of CD69

CD25

and CD71 on T-cell surface were analyzed using flow cytometry. The level of interleukin-2 (IL-2) in the culture supernatant of activated T cells was quantified by enzyme-linked immunosorbent assay (ELISA). The level of phosphorylated IκB-α (p-IκB-α) in total protein and p65

a subunit of NF-κB

nuclear translocation were measured by Western blot analysis. UA in a dose-dependent manner significantly decreased the proliferation and inhibited the surface expressions of CD69

CD25

and CD71 in murine T lymphocytes upon in vitro activation (P<0.01). Significant reduction of IL-2 production was found in activated T cells treated with UA (P<0.01). The PMA-induced increase in p-IκB-α protein was inhibited

and nuclear translocation of p65 from the cytoplasm was blocked by UA. UA is a potent inhibitor for T cell activation and proliferation; these effects are associated with the inhibition of NF-κB signaling pathway.

Abstract

To investigate the effects of ursolic acid (UA) on T-cell proliferation and activation

CD25

a subunit of NF-κB

nuclear translocation were measured by Western blot analysis. UA in a dose-dependent manner significantly decreased the proliferation and inhibited the surface expressions of CD69

CD25

关键词

Ursolic AcidT-cell activationCD69CD25CD71nuclear factor-κB

Keywords

Ursolic AcidT-cell activationCD69CD25CD71nuclear factor-κB

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