Xiongshao Capsule (芎芍胶囊) promotes angiogenesis of HUVEC via enhancing cell proliferation and up-regulating the expression of bFGF and VEGF

Jiu-mao Lin; Jin-yan Zhao; Qun-chuan Zhuang; Zhen-feng Hong; Jun Peng

doi:10.1007/s11655-011-0895-8

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Xiongshao Capsule (芎芍胶囊) promotes angiogenesis of HUVEC via enhancing cell proliferation and up-regulating the expression of bFGF and VEGF

Updated：2021-08-27

- Xiongshao Capsule (芎芍胶囊) promotes angiogenesis of HUVEC via enhancing cell proliferation and up-regulating the expression of bFGF and VEGF
- Chinese Journal of Integrative Medicine Vol. 17, Issue 11, Pages: 840-846(2011)
- Affiliations：
  
  1. Fujian Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the Developmental Fund of CHEN Ke-ji Integrative Medicine (No. CKJ 2008056 and CKJ 2010019)
- DOI：10.1007/s11655-011-0895-8
  CLC：
- Published：2011，
  
  Published Online：6 November 2011，
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Lin, Jm., Zhao, Jy., Zhuang, Qc. et al. Xiongshao Capsule (芎芍胶囊) promotes angiogenesis of HUVEC via enhancing cell proliferation and up-regulating the expression of bFGF and VEGF., Chin. J. Integr. Med. 17, 840–846 (2011). https://doi.org/10.1007/s11655-011-0895-8

Jiu-mao Lin, Jin-yan Zhao, Qun-chuan Zhuang, et al. Xiongshao Capsule (芎芍胶囊) promotes angiogenesis of HUVEC via enhancing cell proliferation and up-regulating the expression of bFGF and VEGF. [J]. Chinese Journal of Integrative Medicine 17(11):840-846(2011)
Lin, Jm., Zhao, Jy., Zhuang, Qc. et al. Xiongshao Capsule (芎芍胶囊) promotes angiogenesis of HUVEC via enhancing cell proliferation and up-regulating the expression of bFGF and VEGF., Chin. J. Integr. Med. 17, 840–846 (2011). https://doi.org/10.1007/s11655-011-0895-8 DOI：

Jiu-mao Lin, Jin-yan Zhao, Qun-chuan Zhuang, et al. Xiongshao Capsule (芎芍胶囊) promotes angiogenesis of HUVEC via enhancing cell proliferation and up-regulating the expression of bFGF and VEGF. [J]. Chinese Journal of Integrative Medicine 17(11):840-846(2011) DOI： 10.1007/s11655-011-0895-8.

摘要

To evaluate the angiogenic effect of the Xiongshao capsule (芎芍胶囊

XSC) in human umbilical vein endothelial cells (HUVEC)

and to investigate the possible molecular mechanisms mediating its biological effect. Serum pharmacology was applied in this study

in which different doses of XSC were administrated to rats orally and then XSC-containing serum (XSC-S) was collected for the following in vitro experiments. The viability of HUVEC was determined by the 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay. Cell density was observed via phase-contrast microscopy. Fluorescence-activated cell sorting analysis with propidium iodide staining was performed to determine cell cycle phase. Cell migration was determined by wound-healing method. Capillary tube formation by HUVEC was examined using ECMatrix gel-based assay. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) expression levels were measured by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbant assay (ELISA) analyses. XSC-S dose-dependently stimulated proliferation of HUVEC by promoting the cell cycle G1 to S progression. In addition

XSC-S treatment dramatically increased the migration and capillary tube formation of HUVEC in a dose-dependent manner. Moreover

XSC-S enhanced the expression of VEGF and bFGF at both mRNA and protein levels. XSC can promote several features of angiogenesis in endothelial cells through up-regulating the expression of bFGF and VEGF

suggesting that XSC may be a potential novel therapeutic agent for the treatment of ischemic heart diseases.

Abstract

To evaluate the angiogenic effect of the Xiongshao capsule (芎芍胶囊

XSC) in human umbilical vein endothelial cells (HUVEC)

and to investigate the possible molecular mechanisms mediating its biological effect. Serum pharmacology was applied in this study

5-dimethylthiazol-2-yl)-2

XSC-S treatment dramatically increased the migration and capillary tube formation of HUVEC in a dose-dependent manner. Moreover

suggesting that XSC may be a potential novel therapeutic agent for the treatment of ischemic heart diseases.

关键词

Xiongshao capsuleChinese Medicineangiogenesisserum pharmacology

Keywords

Xiongshao capsuleChinese Medicineangiogenesisserum pharmacology

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Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine

The Second Affiliated Hospital, Fujian University of Traditional Chinese Medicine

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Key Labatory of Chinese Gan of State Administration of Traditional Chinese Medicien

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