Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism

Ying-dong Li; Yan-hua Ma; Jian-xiong Zhao; Xin-ke Zhao

doi:10.1007/s11655-011-0897-6

Your Location：

Home >

Browse articles >

Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism

Updated：2021-08-27

- Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism
- Chinese Journal of Integrative Medicine Vol. 17, Issue 11, Pages: 854-859(2011)
- Affiliations：
  
  1. Gansu College of Traditional Chinese Medicine,Lanzhou,China
  2. Graduate Institute of Integration Chinese and Western Medicine, Lanzhou University,Lanzhou,China
- Author bio：
- Funds：
  
  Supported by the Key Projects of the National Science and Technology Pillar Program during the Eleventh Five-Year Plan Period (No. 2007BAI37B01) and Major Science and Technology Program of Gansu Province (No. 092NKDA017)
- DOI：10.1007/s11655-011-0897-6
  CLC：
- Published：2011，
  
  Published Online：6 November 2011，
Scan for full text
Li, Yd., Ma, Yh., Zhao, Jx. et al. Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism., Chin. J. Integr. Med. 17, 854–859 (2011). https://doi.org/10.1007/s11655-011-0897-6

Ying-dong Li, Yan-hua Ma, Jian-xiong Zhao, et al. Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism. [J]. Chinese Journal of Integrative Medicine 17(11):854-859(2011)
Li, Yd., Ma, Yh., Zhao, Jx. et al. Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism., Chin. J. Integr. Med. 17, 854–859 (2011). https://doi.org/10.1007/s11655-011-0897-6 DOI：

Ying-dong Li, Yan-hua Ma, Jian-xiong Zhao, et al. Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism. [J]. Chinese Journal of Integrative Medicine 17(11):854-859(2011) DOI： 10.1007/s11655-011-0897-6.

摘要

To investigate whether the administration of the ultra-filtration extract from Danggui Buxue Decoction (当归补血汤

EDBD) was able to protect cardiomyocytes from oxidative injury of rats induced by hydrogen peroxide (H2O2) and its potential mechanism. Myocardial cells from 1- to 2-day-old neonatal rats were cultured in Dulbecco’s modified Eagle’s medium low-glucose and Ham’s F12 medium (1:1)

and the cellular injury was induced by H2O2. The ultra-filtration extract mixture from Angelica sinensis and Hedysarum polybotrys was given in three doses of 3.75

7.5

and 15 mg/mL. Morphological changes of cardiomyocytes were observed by microscope. Survival rate of myocardial cells was assessed using 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay. The cardiomyocyte damages were estimated by detecting lactate dehydrogenase (LDH) and creatine kinase (CK) releases in the medium

superoxide dismutase (SOD) activities

and intracellular malondialdehyde (MDA) and myeloperoxidase (MPO) contents. The levels of caspase-3 and heat shock protein 70 (hsp70) mRNA expression in cardiomyocytes were measured by reverse transcription polymerase chain reaction. The EDBD could protect the cardiomyocytes from H2O2 injury in a dosedependent manner (3.75

7.50

and 15.00 mg/mL). The EDBD could significantly decrease LDH and CK leakages and intracellular MDA and MPO contents

increase SOD activity

up-regulate hsp70 expression

and down-regulate caspase-3 expression. The EDBD has protection on cardiomyocytes injured by H2O2 through improving cell antioxidant ability

up-regulating hsp70 expression

and inhibiting caspase-3 activity.

Abstract

To investigate whether the administration of the ultra-filtration extract from Danggui Buxue Decoction (当归补血汤

and the cellular injury was induced by H2O2. The ultra-filtration extract mixture from Angelica sinensis and Hedysarum polybotrys was given in three doses of 3.75

7.5

and 15 mg/mL. Morphological changes of cardiomyocytes were observed by microscope. Survival rate of myocardial cells was assessed using 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay. The cardiomyocyte damages were estimated by detecting lactate dehydrogenase (LDH) and creatine kinase (CK) releases in the medium

superoxide dismutase (SOD) activities

7.50

and 15.00 mg/mL). The EDBD could significantly decrease LDH and CK leakages and intracellular MDA and MPO contents

increase SOD activity

up-regulate hsp70 expression

and down-regulate caspase-3 expression. The EDBD has protection on cardiomyocytes injured by H2O2 through improving cell antioxidant ability

up-regulating hsp70 expression

and inhibiting caspase-3 activity.

关键词

ultra-filtration extract from Danggui Buxue Decoction (当归补血汤)oxidative injurycardiomyocyteheat shock protein 70

Keywords

ultra-filtration extract from Danggui Buxue Decoction (当归补血汤)oxidative injurycardiomyocyteheat shock protein 70

references

Li GR, Lu CY, Yang SY. Study on Isolation of Angelica Sinensis Dliv. Polysaccharide and its effects on anti-active oxygen free radicals. J Central-south Institute Technol (Chin) 2002;20:747–749.

Deng HJ, Guo YS, Qu YL, Yang XH, Qin L, Wei YM. A study of scavenging action of radix Angelica sinesis recen, wine-Angelica sinesis and oil-Angelica sinesis on oxygen free radical in vivo. Chin Tradit Herbal Drugs (Chin) 2009;40:784–787.

Shang P, Qian AR, Yang TH, Jia M, Mei QB, Cho CH, Zhao WM, Chen ZN. Experimental study of anti-tumor effects of polysaccharides from Angelica sinensis. World J Gastroenterol 2003;9:1963–1967.

Yao BT, ZHAO JX, Wang XX, Lei FF. Experimental study on anti-tumor activity of total hedysarum polybotrys saccharide in vivo. China Tradit Chin Med Pharm (Chin) 2008;23:627–629.

Li YZ, Huang J, Guo HC, Ren B. Chemical constituents from Hedysarum polybotrys and their antitumor activities. Chin Tradit Herbal Drugs (Chin) 2009;40:1195–1198.

Sun YL, Tang J, Gu XH, Li DY. Water-soluble polysaccharides from Angelica sinensis (Oliv.) Diels: preparation, characterization and bioactivity. Int J Biol Macromol 2005;36:283–289.

Guo LW. Significant demand and key problems of membrane science and technology applying to pharmaceutical industry of Chinese material medica. Chin Tradit Herbal Drugs (Chin) 2009;40:1849–1855.

Cao YT, Guo LW, Shi DL, Pan LM, Zhu HX. Advances in studies on ceramic membrane applied in Chinese material medica refining. Chin Tradit Herbal Drugs (Chin) 2010;41:314–317.

Dong Y, Gao RC, Pan Q, Yan XN. Ultrafiltration and nanofiltration membrane separation technology in extracting and purifying oligosaccharides of Rehmannia glutinosa. Chin Tradit Herbal Drugs (Chin) 2008;39:359–363.

Chen SH. A study of using UF in refining Chinese traditional medicine. Water Purific Technol (Chin) 2004;23(2):16–18.

Li YD, ed. An experimental study on angiogenesis promoting effect of the ultra-filtration extract from Angelica Sinensis, Hedysarum Polybotrys and their mixture in ischemic myocardium. Nanjing: Nanjing University of Chinese Medicine; 2005:9–10.

Simpson P, Savion S. Differentiation of rat myocytes in single cell cultures with and without proliferating nonmyocardialcell. Circ Res 1982;50:101–116.

Xiao WM, Jiang BM, Shi YZ, Liu MD, Tang DL, Xin K, et al. Heat shock proteins protect cardiomyocytes against apoptosis induced by hydrogen peroxide through interference with two signal transduction pathways. Chin J Arterioscl (Chin) 2003;11:283–286.

Zhang HB, Wan YT, Wang LZ, Liao CL, Yang HB, Chen LY. Protection and regulation of Hsp70 in cellular stress responses. China Pract Med 2009;4:228–229.

Latchman DS. Heat shock proteins and cardiac protection. Cardiovasc Res 2001; 51: 637–646.

Jiang B, Xiao W, Shi Y, Liu MD, Xiao XZ. Heat shock pretreatment inhibited the release of Smac/DIABLO from mitochondria and apoptosis induced by hydrogen peroxide in cardiomyocytes and C2C12 myogenic cells. Cell Stress Chaper 2005;10:252–262.

Faubel S, Edelstein CL. Caspases as drug targets in ischemic organ injury. Curr Drug Targets Immune Endocr Metabol Disord 2005;5:269–287.

Shen J, Lee W, Gu Y, Tong Y, feng PC, Tong L. Ginkgo biloba extract (EGb761) inhibits mitochondria-dependent caspase pathway and prevents apoptosis in hypoxiareoxygenated cardiomyocytes. Chin Med 2011;23(6):8.

Misra MK, Sarwat M, Bhakuni P, Tuteja R, Tuteja N. Oxidative stress and ischemic myocardial syndromes. Med Sci Monit 2009;15:209–219.

Orrenius S. Reactive oxygen species in mitochondriamediated cell death. Drug Metab Rev 2007;39:443–455.

FULL TEXT LINK

More>

Views

347

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Effects of acupuncture at Baihui (DU20) and Zusanli (ST36) on the expression of heat shock protein 70 and tumor necrosis factor α in the peripheral serum of cerebral ischemia-reperfusion-injured rats

Exploring molecular mechanism underlying Chinese medicine syndrome: A study on correlation between Chinese medicine syndrome and biomarkers for ischemic stroke

Extract of Paris polyphylla Simth protects cardiomyocytes from anoxia-reoxia injury through inhibition of calcium overload

Effects of serum containing Xinlikang on angiotensin II induced hypertrophy in cultured neonatal rat cardiomyocytes

Antioxidative effect of luteolin pretreatment on simulated ischemia/reperfusion injury in cardiomyocyte and perfused rat heart

Related Author

No data

Related Institution

Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences

Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences

Department of Neurology, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine

The Laboratory Research Center of Xiyuan Hospital, China Academy of Chinese Medical Sciences

Yunnan Pharmaceutical Institute

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰