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Effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae on bone marrow stem cells directional homing to necrosis area after osteonecrosis of the femoral head in rabbit
- Chinese Journal of Integrative Medicine Vol. 18, Issue 10, Pages: 761-768(2012)
- Affiliations：
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing,China
2. Wangjing Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：Supported by the National Natural Science Foundation of China (No. 30672770)
DOI：10.1007/s11655-011-0940-7
CLC：Published：2012，
Published Online：12 January 2012，
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Kong, Xy., Wang, Rt., Tian, N. et al. Effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae on bone marrow stem cells directional homing to necrosis area after osteonecrosis of the femoral head in rabbit., Chin. J. Integr. Med. 18, 761–768 (2012). https://doi.org/10.1007/s11655-011-0940-7
Xiang-ying Kong, Rong-tian Wang, Neng Tian, et al. Effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae on bone marrow stem cells directional homing to necrosis area after osteonecrosis of the femoral head in rabbit. [J]. Chinese Journal of Integrative Medicine 18(10):761-768(2012)
Kong, Xy., Wang, Rt., Tian, N. et al. Effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae on bone marrow stem cells directional homing to necrosis area after osteonecrosis of the femoral head in rabbit., Chin. J. Integr. Med. 18, 761–768 (2012). https://doi.org/10.1007/s11655-011-0940-7 DOI：
Xiang-ying Kong, Rong-tian Wang, Neng Tian, et al. Effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae on bone marrow stem cells directional homing to necrosis area after osteonecrosis of the femoral head in rabbit. [J]. Chinese Journal of Integrative Medicine 18(10):761-768(2012) DOI： 10.1007/s11655-011-0940-7.
摘要
To investigate the effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae (Ach) on bone marrow stem cells (BMSCs) homing to necrosis area after osteonecrosis of the femoral head (ONFH) frozen by liquid nitrogen in rabbit as well as to explore the mechanism of prevention and treatment for ONFH. The animal model of ONFH was established by liquid nitrogen frozen on the rabbit left hind leg. Forty-eight Japanese White rabbits were randomly assigned to sham-operated group
model group
Huogu II group
and Huogu II plus Ach group
with 12 rabbits in each. During the course of ONFH animal model establishment
all rabbits were subcutaneously injected with recombinant human granulocyte colony-stimulating factor [rhG-CSF
30 μg/(kg·day) for continuous 7 days]. Meanwhile
normal saline and decoction of the two formulae were administrated by gavage
respectively. White blood cells (WBC) were counted in peripheral blood before and after injection of rhG-CSF. Materials were drawn on the 2nd and 4th weeks after model built; bone glutamine protein (BGP) and bone morphogenetic protein 2 (BMP2) levels in serum were tested. Histopathologic changes were observed by hematoxylin and eosin (HE) staining. BMP2 mRNA levels were detected with in situ hybridization (ISH) staining. 5-Bromo-2′-deoxyuridine (BrdU) and stromal cell derived factor 1 (SDF-1) were measured by immunohistochemical assay in femoral head of the left hind leg. Compared with the shamoperated group
the ratio of empty lacuna
serum BGP
and SDF-1 level in the model group increased significantly
and BMP2 in both serum and femoral head decreased significantly. However
in comparison with the model group
the empty lacuna ratio of Huogu II group and Huogu II plus Ach group decreased obviously in addition to the levels of serum BGP and BMP2
and the expressions of BMP2 mRNA
BrdU
and SDF-1 increased significantly. Above changes were particularly obvious in Huogu II plus Ach group. BGP and SDF-1 on the 2nd week and empty lacuna rate and serum BMP2 level on the 4th week in Huogu II group significantly exceeded their counterparts. On the 2nd week
only in Huogu II plus Ach group that the BrdU counting rose significantly. On the 4th week
empty lacuna rate and serum BMP2 level in Huogu II plus Ach group exceeded those in Huogu II group distinctively. To a certain extent
the medicinal guide Ach improves the preventive and therapeutic effects of Huogu II Formula on experimental ONFH model. The possible mechanism of this is related to its promoting effect on directional homing of BMSCs to the necrosis area.
Abstract
To investigate the effect of Huogu II Formula (活骨II方) with medicinal guide Radix Achyranthis Bidentatae (Ach) on bone marrow stem cells (BMSCs) homing to necrosis area after osteonecrosis of the femoral head (ONFH) frozen by liquid nitrogen in rabbit as well as to explore the mechanism of prevention and treatment for ONFH. The animal model of ONFH was established by liquid nitrogen frozen on the rabbit left hind leg. Forty-eight Japanese White rabbits were randomly assigned to sham-operated group
model group
Huogu II group
and Huogu II plus Ach group
with 12 rabbits in each. During the course of ONFH animal model establishment
all rabbits were subcutaneously injected with recombinant human granulocyte colony-stimulating factor [rhG-CSF
30 μg/(kg·day) for continuous 7 days]. Meanwhile
normal saline and decoction of the two formulae were administrated by gavage
respectively. White blood cells (WBC) were counted in peripheral blood before and after injection of rhG-CSF. Materials were drawn on the 2nd and 4th weeks after model built; bone glutamine protein (BGP) and bone morphogenetic protein 2 (BMP2) levels in serum were tested. Histopathologic changes were observed by hematoxylin and eosin (HE) staining. BMP2 mRNA levels were detected with in situ hybridization (ISH) staining. 5-Bromo-2′-deoxyuridine (BrdU) and stromal cell derived factor 1 (SDF-1) were measured by immunohistochemical assay in femoral head of the left hind leg. Compared with the shamoperated group
the ratio of empty lacuna
serum BGP
and SDF-1 level in the model group increased significantly
and BMP2 in both serum and femoral head decreased significantly. However
in comparison with the model group
the empty lacuna ratio of Huogu II group and Huogu II plus Ach group decreased obviously in addition to the levels of serum BGP and BMP2
and the expressions of BMP2 mRNA
BrdU
and SDF-1 increased significantly. Above changes were particularly obvious in Huogu II plus Ach group. BGP and SDF-1 on the 2nd week and empty lacuna rate and serum BMP2 level on the 4th week in Huogu II group significantly exceeded their counterparts. On the 2nd week
only in Huogu II plus Ach group that the BrdU counting rose significantly. On the 4th week
empty lacuna rate and serum BMP2 level in Huogu II plus Ach group exceeded those in Huogu II group distinctively. To a certain extent
the medicinal guide Ach improves the preventive and therapeutic effects of Huogu II Formula on experimental ONFH model. The possible mechanism of this is related to its promoting effect on directional homing of BMSCs to the necrosis area.
关键词
osteonecrosis of the femoral headbone marrow stem cellshomingguide drug to affected area
Keywords
osteonecrosis of the femoral headbone marrow stem cellshomingguide drug to affected area
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