Protective effect of sodium tanshinone IIA sulfonate on injury of small intestine in rats with sepsis and its mechanism

Wei Zhu; Qing Lu; Hua-wen Chen; Jun Feng; Lei Wan; Dai-xing Zhou

doi:10.1007/s11655-011-0942-5

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Protective effect of sodium tanshinone IIA sulfonate on injury of small intestine in rats with sepsis and its mechanism

Updated：2021-08-27

- Protective effect of sodium tanshinone IIA sulfonate on injury of small intestine in rats with sepsis and its mechanism
- Chinese Journal of Integrative Medicine Vol. 18, Issue 7, Pages: 496-501(2012)
- Affiliations：
  
  1. Department of Emergency Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan,China
  2. Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology,Wuhan,China
- Author bio：
- Funds：
  
  Supported by a Grant from Hubei Province Science and Technique Foundation (No. 2009CDB371)
- DOI：10.1007/s11655-011-0942-5
  CLC：
- Published：2012，
  
  Published Online：9 February 2012，
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Zhu, W., Lu, Q., Chen, Hw. et al. Protective effect of sodium tanshinone IIA sulfonate on injury of small intestine in rats with sepsis and its mechanism., Chin. J. Integr. Med. 18, 496–501 (2012). https://doi.org/10.1007/s11655-011-0942-5

Wei Zhu, Qing Lu, Hua-wen Chen, et al. Protective effect of sodium tanshinone IIA sulfonate on injury of small intestine in rats with sepsis and its mechanism. [J]. Chinese Journal of Integrative Medicine 18(7):496-501(2012)
Zhu, W., Lu, Q., Chen, Hw. et al. Protective effect of sodium tanshinone IIA sulfonate on injury of small intestine in rats with sepsis and its mechanism., Chin. J. Integr. Med. 18, 496–501 (2012). https://doi.org/10.1007/s11655-011-0942-5 DOI：

Wei Zhu, Qing Lu, Hua-wen Chen, et al. Protective effect of sodium tanshinone IIA sulfonate on injury of small intestine in rats with sepsis and its mechanism. [J]. Chinese Journal of Integrative Medicine 18(7):496-501(2012) DOI： 10.1007/s11655-011-0942-5.

摘要

To explore the protective effect of sodium tanshinone IIA sulfonate (STS) on small: intestine injury in rats with sepsis and its possible mechanism. According to a random number table

24 Tats were randomly divided into 3 groups: sham operation group (sham group)

sepsis model group (model group) and STS treatment group (STS group)

with 8 Tats in each group. A rat model of sepsis was induced by cecal ligation and puncture (CLP) for 5 h. STS (1 mg/kg) was slowly injected through the right external jugular vein after CLP. The histopathologic changes in the intestine tissue were observed under a light microscope

and the intestinal epithelial cell apoptosis was evaluated by terminal deoxynucleoddyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) method. The expressions of Bcl-2

Bax and nuclear factor κB (NF-κB) p65 in the intestinal tissue was determined by Western blot. The levels of tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) in the intestinal tissue were determined using enzyme-linked immuno-sorbent assay (ELISA). Obvious injuries were observed in the intestinal tissue in the CLP group compared with the sham group. The expression of NF-κB p65 and the levels of TNF-α and IL-6 were up-regulated after CLP

the apoptosis of intestinal epithelial cells was increased after CLP

and the ratio of Bcl-2 to Bax was decreased. STS post-treatment could attenuate the injury on the intestinal tissue induced by CLP

decrease the apoptosis of intestinal treatment epithelial cells and the levels of NF-κB p65

TNF-α and IL-6

and increase the ratio of Bcl-2 to Bax. STS can protect the small intestine in rats with sepsis

and the mechanism may be associated with the inhibition of intestinal epithelial apoptosis and the reduction of activation of inflammatory cytokines.

Abstract

To explore the protective effect of sodium tanshinone IIA sulfonate (STS) on small: intestine injury in rats with sepsis and its possible mechanism. According to a random number table

24 Tats were randomly divided into 3 groups: sham operation group (sham group)

sepsis model group (model group) and STS treatment group (STS group)

and the intestinal epithelial cell apoptosis was evaluated by terminal deoxynucleoddyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) method. The expressions of Bcl-2

the apoptosis of intestinal epithelial cells was increased after CLP

and the ratio of Bcl-2 to Bax was decreased. STS post-treatment could attenuate the injury on the intestinal tissue induced by CLP

decrease the apoptosis of intestinal treatment epithelial cells and the levels of NF-κB p65

TNF-α and IL-6

and increase the ratio of Bcl-2 to Bax. STS can protect the small intestine in rats with sepsis

and the mechanism may be associated with the inhibition of intestinal epithelial apoptosis and the reduction of activation of inflammatory cytokines.

关键词

sodium tanshinone IIA sulfonatesepsisapoptosisBcl-2Baxnuclear factor κBtumor necrosis factor αinterleukin 6

Keywords

sodium tanshinone IIA sulfonatesepsisapoptosisBcl-2Baxnuclear factor κBtumor necrosis factor αinterleukin 6

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