Carboxymethylpachymaran enhances immunologic function of dendritic cells cultured in two kinds of hepatoma carcinoma cell line’s supernatant via nuclear factor κB/Rel pathway

Zhuo Chen; Bin Yu; Xian-lin Wu; Cong-qi Dai; Guo-qiang Qian; Jian-zhong Yu; Hai-bin He; Zhi-xin Wang; Jun Hou; Xiao-yin Chen

doi:10.1007/s11655-011-0943-4

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Carboxymethylpachymaran enhances immunologic function of dendritic cells cultured in two kinds of hepatoma carcinoma cell line’s supernatant via nuclear factor κB/Rel pathway

Updated：2021-08-27

- Carboxymethylpachymaran enhances immunologic function of dendritic cells cultured in two kinds of hepatoma carcinoma cell line’s supernatant via nuclear factor κB/Rel pathway
- Chinese Journal of Integrative Medicine Vol. 18, Issue 3, Pages: 203-208(2012)
- Affiliations：
  
  1. Medical College, Jinan University,Guangzhou,China
  2. Nursing Department, Huanghuai University,Henan Province,Zhumadian,China
  3. Tongrentang Hospital,Beijing,China
  4. Heshan People Hospital,Guangzhou,China
  5. Dongpu Branch of the First Affiliated Hospital of Jinan University,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30973693) and the Research Fund for the Doctoral Program of Higher Education of China (No. 20104401110003);These authors contributed equally to this work
- DOI：10.1007/s11655-011-0943-4
  CLC：
- Published：2012，
  
  Published Online：2 April 2012，
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Chen, Z., Yu, B., Wu, Xl. et al. Carboxymethylpachymaran enhances immunologic function of dendritic cells cultured in two kinds of hepatoma carcinoma cell line’s supernatant via nuclear factor κB/Rel pathway., Chin. J. Integr. Med. 18, 203–208 (2012). https://doi.org/10.1007/s11655-011-0943-4

Zhuo Chen, Bin Yu, Xian-lin Wu, et al. Carboxymethylpachymaran enhances immunologic function of dendritic cells cultured in two kinds of hepatoma carcinoma cell line’s supernatant via nuclear factor κB/Rel pathway. [J]. Chinese Journal of Integrative Medicine 18(3):203-208(2012)
Chen, Z., Yu, B., Wu, Xl. et al. Carboxymethylpachymaran enhances immunologic function of dendritic cells cultured in two kinds of hepatoma carcinoma cell line’s supernatant via nuclear factor κB/Rel pathway., Chin. J. Integr. Med. 18, 203–208 (2012). https://doi.org/10.1007/s11655-011-0943-4 DOI：

Zhuo Chen, Bin Yu, Xian-lin Wu, et al. Carboxymethylpachymaran enhances immunologic function of dendritic cells cultured in two kinds of hepatoma carcinoma cell line’s supernatant via nuclear factor κB/Rel pathway. [J]. Chinese Journal of Integrative Medicine 18(3):203-208(2012) DOI： 10.1007/s11655-011-0943-4.

摘要

To study the immunologic function of dendritic cells (DCs) cultured in two kinds of hepatoma cell line’s supernatant and the enhancing effects of carboxymethylpachymaran (CMP) on DCs. DCs were harvested after stimulation by granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 from umbilical cord blood using density-gradient centrifugation method. Cultured supernatant of two hepatoma cell lines (HepG2 and HepG2.2.15) were collected for condition medium (CM) according to a volume ratio of supernatant to incomplete RPMI-1640 medium

which was 3:1. CMP was dissolved in incomplete RPMI-1640 medium. Experimental groups were divided according to the culture medium

either CM or with CMP in it. DCs subsets CD83

CD86

CD1a

and d-related human leukocyte antigens (HLA-DR) were analyzed by flow cytometry. The proliferation ability of allogeneic T cells in mixed lymphocyte reaction (MLR) stimulated by DCs was examined by 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) analysis. IL-12p70

interferon-γ (IFN-γ)

and nuclear factor κB (NF-κB) were detected by enzyme-linked immunosorbent assay analysis. The proliferation of lymphocytes and secreting level of IL-12 and expression of phenotype of DCs cultured in two kinds of CM were lower than those of normal group (P <0.01). Compared with the normal group

groups treated with CMP showed a higher expression level of DCs subsets

lymphocyte reproductive activity

as well as IL-12 and IFN-γ secretion levels. Groups treated with CMP also demonstrated higher levels of DCs phenotype expression and IL-12 and IFN-γ secretion in supernatant of MLR and higher lymphocyte reproductive activity compared with CM group (P <0.05). Compared with the normal group

the expression level of NF-κB in DCs nuclear was higher in CMP groups but lower in two CM groups (P <0.05). After CMP was added

the NF-κB expression levels of two CM groups were increased compared with levels before CMP was added (P <0.05). However

there was no significant difference between the two CM groups (P >0.05). Two kinds of hepatoma cell line’s supernatant can inhibit the immunologic function of DCs. This suppressive effect may be related to the inhibition of NF-κB/Rel pathway. CMP may up-regulate the DCs function by activating the NF-κB/Rel pathway.

Abstract

which was 3:1. CMP was dissolved in incomplete RPMI-1640 medium. Experimental groups were divided according to the culture medium

either CM or with CMP in it. DCs subsets CD83

CD86

CD1a

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) analysis. IL-12p70

interferon-γ (IFN-γ)

groups treated with CMP showed a higher expression level of DCs subsets

lymphocyte reproductive activity

the expression level of NF-κB in DCs nuclear was higher in CMP groups but lower in two CM groups (P <0.05). After CMP was added

the NF-κB expression levels of two CM groups were increased compared with levels before CMP was added (P <0.05). However

关键词

dendritic cellscarboxymethylpachymaranhepatocarcinomalymphocyte subsetsmixed leukocyte reaction

Keywords

dendritic cellscarboxymethylpachymaranhepatocarcinomalymphocyte subsetsmixed leukocyte reaction

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Department of Traditional Chinese Medicine, School of Medicine, Jinan University

Shanghai Cancer Center and Shanghai Medical College, Fudan University

Department of Microbiology and Immunology, School of Medicine, Jinan University

Shandong Provincial Corps Hospital of Chinese People’s Armed Police Forces

Department of Obstetrics and Gynecology, First Affiliated Hospital of Jinan University

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